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Masaru Kojima Yasuo Hosomura Hideaki Itoh Takashi Johshita Yoshihiro Ohno Katsue Yoshida Shigeyuki Asano Haruki Wakasa Shigeo Nakamura Taizan Suchi 《Pathology international》1991,41(5):363-368
In order to clarify the appearance of monocytoid B lymphocytes (MBLs) in abscess-forming granulomatous lymphadenitis (AGL) and the relation between AGL and cat-scratch disease (CSD), 48 cases of AGL were studied histologically. MBLs were present in about 50% of AGL cases. Warthin Starry (WS) silver stain-positive bacteria, which are the causative agent of CSD, were present in 52.4% of AGL cases with MBLs and 59.2% of AGL cases without MBLs. The appearance of MBLs in AGL was not related to various clinical features, including disease interval from initial lymphadenopathy to lymph node biopsy. Histologically, epithelioid cell clusters appeared in about 70% of MBL positive AGL cases, but were not observed in MBL negative AGL. Therefore, a close interaction between MBLs and epithelioid cells in AGL is suggested, and we emphasize that the histological features of some AGL cases resemble those of toxoplasmic lymphadenitis. 相似文献
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虽然目前分子技术在临床病理诊断领域中取得了广泛应用,但许多传统的特殊染色法仍是检测病原体不可缺少的方法.其中,Warthin-Starry(W-S法)染色就是一种经典的特染方法.W-S法首先在1920年由Warthin和Starry[1]作为显示螺旋体的一种特殊染色方法提出,其原理是一种嗜银反应.下面我们将结合相关文献及自身体会就该方法的主要环节及综合应用作简要介绍. 相似文献
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Cat scratch disease 总被引:4,自引:0,他引:4
Eiji Kudo Akihiko Sakaki Masayuki Sumitomo Yoshiyuki Fujii Takanori Hirose Toshiaki Sano Kazuo Hizawa 《Virchows Archiv : an international journal of pathology》1988,412(6):563-572
Summary The aetiological agent of cat scratch disease (CSD) has been unknown for more than 30 years. Recently, a micro-organism clearly shown with Warthin-Starry silver (W-S) stain was found and thought to be a possible cause of the disease. In this study, 32 cases of regional lymphadenopathy histologically compatible with CSD and 20 contrasting cases of lymphadenopathy were examined retrospectively with W-S stain. W-S positive pleomorphic organisms were clearly demonstrated in 20 of the 32 suspected cases of CSD, but in none of the other cases. The onset of disease in these 20 cases with W-S positive organisms occurred between July and January. This seasonal variation in the onset of disease was highly significant (P<0.005) and was not due to a single epidemic. Moreover, some characteristic morphological features of the organism were found by electron microscopic observations. Ultrastructurally, the organism was a bacterium showing a chain-like arrangement, septal formation, branching and clubbed ends. 相似文献
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目的比较WarthinStarry(WS)银染色与Grams染色在组织学诊断幽门螺杆菌(Helicobacterpylori,HP)感染中的作用。方法HE染色法判断所选组织的病理分型并进行分组;用WS银染色法结合Grams染色法判断所选组织HP的感染状况,比较两种不同染色方法的染色结果。结果WS银染色结果阳性率为85.5%,Grams染色结果阳性率为87.74%,两者比较差别无统计学意义(P>0.05)。结论WS银染色与Grams染色在组织学诊断HP感染中的作用无显著性差别,Grams染色可用于胃组织石蜡切片中HP感染的检查。 相似文献
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胃黏膜癌前病变中bcl-2蛋白表达与幽门螺杆菌感染的关系 总被引:1,自引:0,他引:1
目的 观察幽门螺杆菌(HP)感染后胃黏膜癌前病变中bcl—2蛋白表达的特点,探讨服在胃癌发生发展过程中的作用。方法 应用免疫组织化学SP法及Warthin-Starry嗜银染色(WS染色),对56例慢性胃炎、46例慢性胃炎伴肠化生、34例异型增生进行bcl—2蛋白表达及服检测的研究。结果 胃黏膜肠化生组及异型增生组的服感染率及bcl—2蛋白阳性表达率均明显高于慢性胃炎组(P<0.05);86例HP阳性者bcl-2蛋白表达39例,阳性率45.3%,50例HP阴性者bcl—2蛋白表达12例,阳性率24%,两者比较,差异显著(P<0.05)。结论 在慢性胃炎、肠上皮化生、异型增生的进展过程中,bcl—2蛋白的表达水平在增高。HP感染在胃癌发生、发展过程中起一定作用。bcl—2蛋白的表达可能是服致癌的作用机制之一。 相似文献
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Yukari T. Tsubota Hirokazu Nakamine Toru Takenaka Koichi Kuribayashi Yoji Kusuyama Koji Saito Jiro Maeda 《Virchows Archiv : an international journal of pathology》1991,418(5):383-386
Summary A histological variant of plasma cells found in the granulomas of cat-scratch disease (CSD) lymphadenitis is reported. Though the lesion shows the typical features of suppurative granulomatous lymphadenitis, many atypical giant cells which have abundant basophilic cytoplasm and bizarre nuclei with occasional multi-nucleated forms are noted among epithelioid histiocytes. The diagnosis of CSD lymphadenitis was confirmed by comparing clinical, histopathological, and histochemical (Warthin-Starry silver impregnation stain) studies on lymph node sections from five cases with features typical of the disease. Histochemical (methyl green-pyronine stain) and immunohistochemical examination provided several lines of evidence indicating that the atypical giant cells in our case were plasmacytic and confirmed that its proliferation was reactive, not neoplastic. Multinucleated giant cells were also occasionally present in the other five cases, but they had histological and immunohistochemical features of Langhans' type giant cells. We stress the importance of distinguishing such atypical large plasma cells from neoplastic cells. 相似文献
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《Journal of histotechnology》2013,36(3):149-151
AbstractA staining method for demonstrating spirochetes and bacteria, which stain poorly with the Gram stain, is described. The method is similar to the Warthin-Starry procedure except that the silver impregnation and reducing steps are done in a microwave oven and an acetic acid-glycine solution is used in place of citric acid. If spirochetes are to be stained, the sections are treated with uranyl nitrate, which is also heated in a microwave oven. The staining results are consistent and reliable. (The J Histotechnol 11: 149, 1988.) 相似文献
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《Journal of histotechnology》2013,36(4):339-340
AbstractA consistent 1procedure for the Warthin-Starry stain technique for melanin and spirochetes is described. Close attention to solution preparation, purity, and pH, freshness of chemicals used, and rigorous adherence to the protocol is essential. The procedures and recommendations described ensure consistent success when using this often difficult stain technique. (The J Histotechnol 19:339–340, 1996) 相似文献