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Shinobu Nomura Hiroko Kojima PhD Yoshimi Ohyabu Kosuke Kuwabara Akihiro Miyauchi PhD Toshimasa Uemura PhD 《Journal of artificial organs》2006,9(2):90-96
Nanopillar sheets were fabricated with high-aspect ratio structures with a diameter of 160–1000 nm and a height of 1 μm by
nanoimprinting. The suitability of nanopillar sheets as a new type of cell culture dish was examined by studying the behavior
of HeLa cells cultured on the sheets using light microscopy, scanning electron microscopy, and fluorescence microscopy observing
actin and vinculin molecules. The nanopillar structure enabled easy subculture of the cells from the sheets without conventional
trypsinization. Moreover, the HeLa cells divided and proliferated on the sheets in a different way to that found on petri
dish because of the manner in which the cells adhered to the materials. 相似文献
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