Expression characteristics of neutrophil and mononuclear-phagocyte related genes mRNA in the stable angina pectoris and acute myocardial infarction stages of coronary artery disease

Objective

To investigate expression differences of neutrophil and mononuclear phagocyte related gene mRNAs among acute myocardial infarction (AMI), stable angina (SA) and control groups, and then discuss their expression characteristics in the stable angina pectoris (SAP) and AMI stages of coronary artery disease (CAD).

Methods

Whole Human Genome Oligo Microarrays were applied to assess the differential expression characteristics of neutrophil and mononuclear phagocyte related mRNAs in patients with AMI (n = 20), SA (n = 20) and controls (n = 20).

Results

(1) Almost all colony-stimulating factors (CSF) and their receptors related mRNAs was up-regulated in AMI and SA groups compared with the control group, and the expression of granulocyte-macrophage colony stimulating factor receptor (GM-CSFR) and granulocyte colony stimulating factor receptor (G-CSFR) mRNAs in the AMI group was significantly up-regulated compared with the other two groups (P < 0.01). (2) The expression of mRNAs related to monocyte chemoattractant protein-1 (MCP-1), CCR2 (MCP-1 receptor) and CXCR2 (IL-8 receptor) was significantly up-regulated (P < 0.01) in AMI group compared with SA and control groups. IL-8 mRNA expression in the AMI group was clearly higher than the controls (P < 0.05). (3) All mRNAs expression related to opsonic receptors (IgG FcR and C3bR/C4bR) was significantly up-regulated in AMI group compared with SA and control group (P < 0.01), and the SA group showed an upward trend compared with controls. (4) Most pattern recognition receptor (PRR)-related mRNAs expression was up-regulated in AMI group compared with SA and control groups. Most toll-like receptor (TLR) mRNAs expression was significantly up-regulated (P < 0.01) than the SA and control groups; macrophage scavenger receptor (MSR) mRNA was significantly up-regulated in AMI group compared with the control group (P < 0.01), and the SA group showed an upward trend compared with the controls.

Conclusions

The expression of most neutrophil and mononuclear-macrophage function related genes mRNAs was significantly up-regulated by stages during the progression of CAD, suggesting that the adhesive, chemotactic and phagocytic functions of neutrophil and mononuclear-macrophage were strengthened in the occurrence and development of coronary atherosclerosis and AMI. This also showed a stepped upward trend as the disease progressed.     

朗格汉斯组织细胞增生症的诊治进展

朗格汉斯组织细胞增生症（LCH）是一种少见的、病因尚不明确的单核-巨噬细胞异常增生性疾病,其临床表现具有多样性,累及多个系统,且发病机制复杂,目前主要认为是炎性髓样肿瘤,治疗上仍以联合化疗为主,随着靶向药物及骨髓移植应用逐渐增多,其并发症及不良反应需进一步解决。对于单系统受累LCH 患者预后较佳,临床报道治愈病例较多。而多系统受累LCH患者预后不佳,病死率高。本文就近年来有关LCH的发病机制、临床表现、诊断与治疗及预后进展进行综述。     

舌鳞癌酸性微环境下雷公藤多甙对单核-巨噬细胞分泌血管内皮生长因子的影响

目的 检测雷公藤多甙(tripterygium wilfordii polyglycosidium,TWP)在模拟舌鳞癌局部酸性微环境下对单核-巨噬细胞分泌血管内皮生长因子(vascular endothelial growth factor,VEGF)的影响.方法 在pH值6.6、6.8酸性微环境中,用对数生长期的Tca8113细胞培养上清液与人单核-巨噬细胞系THP-1细胞混合培养,经不同浓度的TWP干预及干预不同时间,以酶联免疫吸附测定法检测其对THP-1细胞分泌VEGF的影响.结果 THP-1细胞与Tca8113细胞上清液混合培养4h,THP-1细胞分泌VEGF受到抑制,分泌抑制率随TWP浓度增加而增大,与浓度成正相关(pH值6.6时,r=0.983,P＜0.001;pH值6.8时,r=0.971,P＜0.001).pH值为6.6,TWP浓度为5×10-1μg/mL时,分泌VEGF抑制率随时间延长而增大,与时间成正相关(r=0.975,P＜0.05).结论 体外模拟的舌鳞癌酸性微环境中,TWP抑制单核-巨噬细胞分泌VEGF,抑制率分别与药物浓度及作用时间正相关.     

重症肺炎早期诊断标志物研究进展

重症肺炎病死率高,许多非特异性指标可能与重症肺炎的严重程度相关,如C反应蛋白(CRP)、降钙素原(PCT)等,但是这类指标特异性差,不能精准地反应重症肺炎的严重程度。多项研究证实单核-巨噬细胞在免疫应答中的作用,尤其在重症感染中的作用尤为重要。CD40是肿瘤坏死因子受体超家族成员之一,多项研究表明脓毒症患者sCD40水平升高。CD163是目前发现仅在单核-巨噬细胞表达的一种跨膜糖蛋白,是清道夫受体超家族成员之一,重症肺炎患者血清sCD163多有升高。本文以CD40及sCD163为例,研究单核-巨噬细胞相关分子在重症肺炎早期诊断及预后中的作用。     

问号钩端螺旋体侵入单核-巨噬细胞方式及其吞噬泡形成差异性研究

目的 探讨问号钩端螺旋体(简称钩体)侵入人或鼠单核-巨噬细胞方式及其吞噬泡形成差异性.方法 采用透射电镜观察问号钩体黄疸出血群赖型赖株侵入小鼠单核-巨噬样细胞J774A.1和佛波酯(PMA)激活的人单核细胞THP-1后吞噬泡形成情况.采用免疫荧光联合激光共聚焦显微镜及荧光分光光度仪等方法,观察细胞内吞抑制剂单丹磺酰尸胺(MDC)、氧化酚砷(PAO)阻断及内吞相关网格蛋白抗体封闭前后,J774A.1细胞和PMA激活的THP-1细胞内问号钩体赖株数量的变化.结果 J774A.1细胞内问号钩体存在于吞噬泡内,THP-1细胞内问号钩体无吞噬泡膜包绕.MDC和PAO能以剂量依赖方式抑制J774A.1和THP-1细胞内吞问号钩体,其中10 μmol/L以上MDC和1 μmol/L以上PAO阻断的J774A.1和THP-1细胞内问号钩体数量明显少于未阻断细胞(P＜0.05).网格蛋白抗体封闭后,J774A.1和THP-1细胞内问号钩体数量也明显减少(P＜0.05).结论 问号钩体以网格蛋白依赖性内吞途径侵入人或鼠单核-巨噬细胞.人或鼠单核-巨噬细胞内问号钩体吞噬泡形成有明显差异,这可能是人或鼠感染问号钩体后发病情况不同的原因之一.