圆形分布法在蝇类季节消长规律中的应用

目的确定蝇类季节消长的高峰日和高峰时段,为制定蝇类防制措施提供科学依据。方法用圆形分布法统计分析绍兴市区2003-2004年蝇类密度监测数据。结果绍兴市区2003-2004年蝇类平均密度高峰日为8月4日,高峰时段为6月8日至9月30日。其中2003年蝇类密度高峰日为8月11日,高峰时段为6月17日至10月5日;2004年蝇类密度高峰日为7月29日,高峰时段为6月1日至9月26日。结论绍兴市区2年中蝇类消长有明显的季节性规律。     

Maggot homogenate is associated with neural regeneration and wound healing in the rat

BACKGROUND:Live delivery limits the clinical application of maggot therapy. To date in China, there are no in vivo reports regarding wound healing mechanisms of maggot therapy or the effects of maggot homogenate on wound nerve regeneration.OBJECTIVE:To avoid complications due to the use of live maggots, an aseptic maggot homogenate was applied. Substance P (SP) and gene protein product 9.5 expression in a cutaneous wound was analyzed to explore possible mechanisms of neural regeneration and wound healing in the rat.DESIGN, TIME AND SETTING:A random grouping and controlled animal study was performed at the laboratory of the Department of Orthopedic Surgery, First Affiliated Hospital, Dalian Medical University from August 2008 to April 2009.MATERIALS:Live maggots were cultured and provided by the laboratory of the Department of Orthopedic Surgery of the First Affiliated Hospital, Dalian Medical University, China.METHODS:A total of 48 adult rats were selected and two acute, full-thickness wounds (round, 1.5 cm diameter) were created on the back of each rat. The two wounds were randomly assigned to homogenate product and control groups. Following two-step disinfection of maggots, a homogenate was produced from 10 maggots and applied to the wound area in the homogenate product group, while the wounds in the control group were treated with normal saline alone.MAIN OUTCOME MEASURES:On days 1,3, 7, 10, 14, and 21 following injury, the wound tissue was excised. Histological examination of the wound was observed by hematoxylin and eosin staining or Masson's Trichrome staining. SP and protein gene product 9.5 expressions were examined by immunohistochemistry to evaluate wound neural regeneration.RESULTS:On days 7, 10, and 14, the rate of wound healing was significantly greater in the homogenate product group compared with the control group (P < 0.05), and homogenate healing was better than that seen in the control group. On days 3, 7, and 10, SP expression in cells and regenerative nerves was significantly greater in the homogenate product group compared with the control group (P < 0.05). On days 7 and 10, protein gene product 9.5 expression was detected in the regenerative nerve, and expression level was significantly greater in the homogenate product group compared with the control group (P < 0.05).CONCLUSION:Maggot homogenate resulted in upregulated SP and protein gene product 9.5 expressions, thereby promoting neural regeneration and wound healing.     

青岛流亭国际机场口岸蝇类本底调查报告

[目的]掌握青岛机场口岸蝇类本底情况，为口岸控制媒介生物提供科学依据。[方法]2006年5月～2007年12月对青岛机场口岸采用诱蝇笼捕集法，并不定期配合采用人工网捕法进行蝇类和其孳生地的调查。[结果]共捕获蝇类46715只，其中采用诱蝇笼捕集法捕获44685只。经分类鉴定分别属于7科47属97种，优势种为丝光绿蝇。[结论]本次调查全面掌握了青岛机场口岸蝇类的种类组成、季节消长情况和其孳生场所。加强机场周边环境整治，消灭蝇孳生地是今后灭蝇工作的重点。     

乌鲁木齐蝇类名录及分布

本文首次报道乌鲁木齐蝇类调查情况,共捕获有瓣蝇类53属125种。     

湖北荆州市区蝇类区系研究

目的 研究荆州市区蝇类的区系.方法 分析已知蝇类的区系成分.结果 已知蝇类5科39属87种,其中,东洋界为主的种类39种,占44.9%;属古北界为主的种类27种,占31.0%;广布两界的种类21种,占24.1%.结论 荆州市区蝇类的区系应划归为东洋界.     

2007年浙江各口岸蝇类本底调查

[目的]掌握浙江各口岸（宁波口岸除外）蝇类的本底情况,为制定有效的媒介生物防制措施和控制口岸虫媒传染病的发生提供科学依据。[方法]2007年1—12月采用诱蝇笼诱捕法对浙江6个口岸蝇类的种群结构、密度和季节消长情况进行了调查。[结果]共捕获蝇类113073只,经种类鉴定,隶属于7科18属27种。蝇类平均密度为87．65只／笼·天,其中大头金蝇为优势种,占捕获总数的59．77％;其次是家蝇（舍蝇）和丝光绿蝇,分别占11．19％和9．55％。浙江口岸蝇类活动高峰期为7—10月,以9月为最高,密度为176．01只,笼·天。蝇类携带霍乱孤菌检测结果全部为阴性。[结论]此次调查取得浙江各口岸蝇类的本底资料,为采取综合防制措施和进一步开展虫媒传染病监测提供了依据。     

口蹄疫乳鼠毒在蚯蚓和苍蝇中实验带毒的研究

O型口蹄疫(FMD)乳鼠毒注射到蚯蚓(Pheretima sp.)体内后的第8天仍可检测到口蹄疫病毒(FMDV),但第14天以后则未查出。将此病毒洒在蚯蚓生活的土壤中,检测蚯蚓体内FMDV的结果亦同。本试验还间接地表明,FMDV不能在蚯蚓体内复制、增殖,蚯蚓只起携带FMDV的作用。蚯蚓属于低等动物,免疫系统尚未健全,但对非已物质有一定的免疫应答。蚯蚓与FMDV接触后,用间接血凝测得其体腔液中具有“抗体”反应。FMDV与舍蝇属(Muscasp.)和金蝇属(Chrysomyia sp.)的苍蝇接触24小时后,对其带毒检测结果表明:苍蝇只能短时在体表携带FMDV,且苍蝇体表携带FMDV的量很少。故认为在FMDV传播中苍蝇只起短时机械扩散作用。     

新疆蝇类研究Ⅲ.丽蝇科和麻蝇科

本文报告了新疆丽蝇和麻蝇科研究结果。丽蝇科12属28种，新种1个，中国新纪录10个。麻蝇科23属73种，中国新纪录属1个，新种8个，中国新纪录21个。