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Yuling Chen Jing Li Deyu Hu Wei Xue Xiaoyan Ding 《Food and Agricultural Immunology》2017,28(5):904-915
Dufulin (DFL) is a potent, new-generation agrochemical that protects crops, such as tobacco, from viruses. Herein, for the first time, we developed a sensitive and specific polyclonal antibody-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) to detect DFL residues in samples. The assay showed high sensitivity and specificity to DFL, with a half maximal inhibitory concentration (IC50) of 48.5?ng?ml?1 and limit of detection (IC20) of 2.5?ng?ml?1. The average recovery of DFL in different spiked samples, such as tobacco, rice, tomato, cucumber, soil and water, was in the range of 70.7–138.8%. A statistically significant correlation was observed between our developed ELISA and previously established methodology of high-performance liquid chromatography with a diode array detector. These results indicate our assay is a potentially useful analytical tool for rapid detection of DFL residue in actual samples. 相似文献
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