The diagnosis and treatment of dentinogenic ghost cell tumor

The purpose of this study is to assess the diagnosis, treatment and prognosis of the dentinogenic ghost cell tumor (DGCT). This is a retrospective study of patients with DGCT who underwent one or multiple surgical interventions during the 10-year period from 1997 to 2007. Seven patients with DGCT were identified under the WHO 2005 classification guidelines. The lesions were located in the maxilla in 4 patients and in the mandible in 3 patients. Five patients experienced recurrences following conservative surgery. They were all treated with aggressive local resection. Two patients had no conservative surgery but aggressive local resection. After 7–105 months’ follow-up, no recurrences were found in any of the patients. Intraosseous DGCT should be treated by resection with an adequate safety margin, of at least 0.5 cm, as recommended for ameloblastoma. Patients with a DGCT should remain in long-term follow-up.     

A simple and rapid method for detection of serum IgM antibodies to the rubella virus hemagglutinin

Serum IgM antibodies directed against the rubella virus hemagglutinin can be detected without prior serum fractionation. In the first step of a newly developed method, chick erythrocytes were sensitized with a subhemagglutinating dose of rubella hemagglutinin. Next, the sensitized erythrocytes were mixed with patient serum, allowing specific antibodies to react with the fixed antigens. Finally, rabbit antibodies to human IgM were used to create bridges between IgM molecules on different blood cells. The visible result was an easily read hemagglutination with sera which contain specific rubella IgM antibodies. The procedure is very simple and rapid to perform. At least 20 sera can be examined in approximately 2 h. No sophisticated instruments are needed.We have tentatively called the new method rubella anti-IgM hemagglutination (HA). Rubella antiIgM HA was more sensitive than the standard density gradient centrifugation/hemagglutination inhibition technique, but the correlation between the methods was good. Non-specific inhibitors of hemagglutination or rheumatoid factors did not seem to interfere with the specificity of the new method, and competition for antigenic sites between antibodies from the IgG/IgA and IgM classes did not seem to represent a serious, practical problem.     

牙本质生成性影细胞瘤的治疗

目的:探讨牙本质生成性影细胞瘤的诊断、治疗,特别是对复发性牙本质生成性影细胞瘤的治疗。方法:回顾性研究了南京市口腔医院自1997-06～2009-06所收治的8例牙本质生成性影细胞瘤患者,手术切除范围视同于成釉细胞瘤,按临界瘤的治疗方法进行,要有0.5 cm的骨质安全缘。结果:发生在上颌骨4例,下颌骨4例。5例患者为复发性牙本质生成性影细胞瘤,扩大切除后,经过10～124个月的随访,所有病例均无复发。结论:牙本质生成性影细胞瘤的治疗类似于成釉细胞瘤,应确保有0.5 cm的骨质安全缘,对于复发性牙本质生成性影细胞瘤,还应保持长期随访。