Allergic conversion of protective mucosal immunity against nasal bacteria in patients with chronic rhinosinusitis with nasal polyposis

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Cefdinir-induced red stool and purple discoloration of nutritional formula: A case report

This case report describes a 4-year-old girl with food aversion who received three separate courses of cefdinir mixed with PediaSure®, an iron-fortified nutritional formula. The patient's stool turned red with variable onset during all three courses of treatment. Moreover, the PediaSure® formula turned purple after mixed with cefdinir, an interaction that has not been previously reported. We recommend that medication counseling for pediatric patients taking cefdinir include a mention of these possible discolorations.     

三元络合物光度法测定微量锌的研究Zn—SCN^- -邻苯二酚紫体系

利用在氨性缓冲溶液中Zn^2＋能与SCN^-和邻苯二酚紫形成三元络合物,建立了一种新的测定锌含量的分光光度法。本法操作简单,选择性好,精密度高。锌浓度在0～2．0g（50ml）时与吸光度呈线性关系。线性回归方程是A=0．002＋0．0836C（g／50m1）,相关系数是r=0．9938,检出限为0．05g／ml,用于水中微量锌的测定,结果令人满意。     

LRP16基因抗紫外线DNA损伤作用的研究

目的根据基因编码序列推测其氨基酸序列，预测LRP16基因编码蛋白的功能并通过实验加以证实。方法利用GeneBank数据库获取LRP16基因序列，然后推测出该基因编码蛋白质的一级结构，对该序列蛋白结构域的同源性进行搜索；将LRP16基因ORF插入pcDNA3．1，构建LRP16基因的真核表达质粒，采用Superfect稳定转染急性粒细胞白血病细胞系HL-60，筛选稳定表达LRP16基因的细胞；以紫外灯照射筛选后有HL-60稳定过表达的HL-60细胞和对照细胞；采用单细胞凝胶电泳技术检测有LRPl6过表达对HL-60细胞增殖及DNA损伤后修复的影响。结果在LRPl6基因理论蛋白序列的第148至315氨基酸残基之间具有与人类组蛋白H2A1C末端相类似的同源序列hismacro、COG2110和A1PP，同源性高达80％以上。LRP16基因的过表达具有抗紫外线引起HL-60细胞DNA损伤和增强损伤后的修复作用。结论LRP16基因编码蛋白可能具有抗紫外线的DNA损伤作用。     

CD8+CD28−CD127loCD39+ regulatory T-cell expansion: A new possible pathogenic mechanism for HIV infection?

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紫外线照射用于骨折术后感染伤口的疗效观察

对19例骨折术后病人感染伤口在常规护理的基础上,配合紫外线照射.结果表明,配合紫外线照射组(Ⅱ组)5天有效率为89.4%,23例常规换 药护理组(Ⅰ组)5天有效率为(30.4%),经统计学处理,X~2=14.81,P<0.05,说明两组之间有差异,提示配合紫外线照射组疗效高于常规护理组.     

Photochemical damage to skin fibroblasts caused by protoporphyrin and violet light

Summary Foreskin fibroblasts cultured in a medium containing protoporphyrin and exposed to violet light lose the capacity to proliferate. This phenomenon can be assessed on the basis of the ability of the irradiated cells to form colonies. Potentially lethal injuries can, however, be repaired during post-irradiation incubation under optimal growth conditions. We investigated the photodynamically induced transformations of certain molecular targets in the irradiated cells. Biochemical analysis showed that only traces of unsaturated fatty acids were oxidized, but SH groups of both the membranes and the cytosol appeared to be very sensitive targets. Of the tryptophan content, 20% was damaged during irradiation. Recovery was observed during post-irradiation incubation. The tryptophan content and the SH groups recovered to some extent, and these results showed a good correlation with the regeneration of surviving cells.     

Sensitization to reactive textile dyes in patients with contact dermatitis

Reactive dyes are used especially for colouring natural fibres (cotton, silk and wool) that arc widely used in Western countries, particularly Italy, in the production of clothes. The aim of our study was to investigate sensitization to the most commonly used reactive textile dyes in patients undergoing patch tests, and to assess the clinical relevance of contact sensitization of these dyes. 1813 consecutive patients underwent patch tests with the GIRDCA standard series and an additional textile series of 12 reactive dyes. 18 of these patients were sensitized to reactive dyes (0.99) (4 only to reactive dyes). The dyes most frequently responsible for positive patch tests were Red Cibacron CR and Violet Remazol 5R (respectively, 8 and 5 positivities). In 5 cases only was a history of intolerance to particular garments given; of 4 patch tests performed with pieces of garment, 2 were positive. In 1 occupationally-exposed patient, airborne contact dermatitis was suspected. Owing to the lack of up-to-date patch test series, some cases of allergic contact dermatitis from textile dyes are probably misdiagnosed: new colouring agents are continuously introduced to the market, so that a close relationship with textile industry is necessary to improve our diagnostic tools.     

原子吸收分光光度法测定柴胡中微量元素

用原子吸收光度法测定并分析了紫胡中Cu、Zn、Fe、Mg、Mn、Ca等微量元素的含量。结果表明,柴胡含有许多和人体健康密切相关的微量元素,具有很高的药用价值。     

LC-MS/MS法同时测定水产品中孔雀石绿、结晶紫及其代谢物残留量

[目的]建立测定水产品中孔雀石绿(MG)、结晶紫(GV)及其代谢产物残留量的液相色谱-串联离子阱质谱仪测定法(LC-MS/MS)。[方法]将样品中残留的MG或GV用硼氢化钾还原为其相应的代谢产物隐色孔雀石绿(LMG)或隐色结晶紫(LGV),经净化预处理,用串联离子阱质谱在电喷雾-全扫描二级质谱(MS/MS)模式进行质谱测定,与HPLC方法测定结果比对验证。[结果]在55件可疑样品中,用本法测定有46件为阳性,9件为假阳性,本法测定结果满意。[结论]采用LC-MS/MS法测定水产品中MG、GV及其代谢产物含量,结果准确,灵敏度高。