常规与微创冠脉搭桥术围手术期血浆S100-B蛋白水平的相关研究

目的: 通过测定体外循环和非体外循环条件下冠脉搭桥术患者血浆S100-B蛋白水平的变化，比较2种手术方式对脑损伤程度有无不同，探讨术中影响血浆S100-B蛋白水平变化的相关因素。方法: 30例择期行冠脉搭桥术的患者，分为体外循环组（CPB group，A组）和微创非体外循环组（non-CPB group，B组）,每组均为15例。于术前、麻醉后、主动脉侧壁钳开放时、术毕时、术后2 h、6 h、12 h和24 h采血测血浆S100-B蛋白浓度。结果： （1） 两组患者血浆S100-B蛋白浓度均在升主动脉侧壁钳开放时显著高于术前，且A组患者血浆S100-B蛋白浓度峰值是B组的3倍多（2.32±0.26 μg/L和0.71±0.14 μg/L）,之后逐渐降低，到术后24 h基本接近正常。（2） 术中患者血浆S100-B蛋白升高水平与体外循环转机时间或心表手术操作时间呈正相关（A组：r＝0.659，P＜0.05;B组：r＝0.584, P＜0.05）。结论： 血浆S100-B蛋白水平可以用来评价2种冠脉搭桥术对脑的损伤程度，术后连续检测对于诊断脑损伤并及时采取相应预防措施具有重要价值;非体外循环微创冠脉搭桥术较常规体外循环下手术更能够有效降低对脑损伤的程度和术后出现神经功能障碍的风险。     

Reactive arthritis after pharyngeal infection: report of two siblings

Abstract

?In this report we describe the cases of two siblings with reactive arthritis (ReA) induced by pharyngeal infections. The patients were a man and his sister living with their parents. He developed arthritis in August 1997, and his younger sister developed similar symptoms in September 1998. Their disease conditions were both severe and required hospitalization. Their conditions improved with the administration of nonsteroidal anti-inflammatory drugs together with antibiotics, and both fully recovered within 1–2 weeks. Rheumatic fever was ruled out since streptococcal infections were not demonstrated with antistreptolysin O (ASO) or antistreptokinase (ASK) titers, or with pharyngeal culture. The sister suffered from a rash which was similar to erythema nodosum on her lower extremities, but neither chorea nor carditis was observed. Both human leukocyte antigen (HLA) typing analyses revealed positive results for HLA-B40 and -B39 for the brother and sister, respectively. Both HLA-B40 and -B39 are considered to be related to HLA-B27-negative ReA, most likely poststreptococcal reactive arthritis (PSRA). Therefore, the two patients were tentatively diagnosed as suffering from PSRA.     

SH2-Bβ在局灶性脑缺血再灌注大鼠顶叶皮质的表达

目的研究信号分子SH2-Bβ在局灶性脑缺血再灌注大鼠大脑顶叶皮质表达情况为阐明脑缺血的演变的分子机制从而为临床治疗提供理论依据。方法用线栓法阻塞大鼠右侧大脑中动脉制作局灶性脑缺血再灌注模型,动物随机分为假手术组和缺血再灌注组,应用免疫组织化学SABC法和图像分析方法检测大鼠顶叶皮质SH2-Bβ表达情况。结果假手术组右侧顶叶皮质SH2-Bβ有很少的表达,而缺血再灌注组3 h时间点SH2-Bβ在右侧顶叶皮质的平均光密度值比假手术组明显增高,持续到24 h,到48 h时间点开始下降,到72 h已逐渐下降但仍高于假手术组(P<0.05)。结论局灶性脑缺血再灌注大鼠顶叶皮质SH2-Bβ被诱导而表达增高,SH2-Bβ可能参与NGF对缺血神经元的保护作用机制,促进神经元存活和损伤的修复。     

Rocaglates as Antivirals: Comparing the Effects on Viral Resistance,Anti-Coronaviral Activity,RNA-Clamping on eIF4A and Immune Cell Toxicity

Rocaglates are potent broad-spectrum antiviral compounds with a promising safety profile. They inhibit viral protein synthesis for different RNA viruses by clamping the 5′-UTRs of mRNAs onto the surface of the RNA helicase eIF4A. Apart from the natural rocaglate silvestrol, synthetic rocaglates like zotatifin or CR-1-31-B have been developed. Here, we compared the effects of rocaglates on viral 5′-UTR-mediated reporter gene expression and binding to an eIF4A-polypurine complex. Furthermore, we analyzed the cytotoxicity of rocaglates on several human immune cells and compared their antiviral activities in coronavirus-infected cells. Finally, the potential for developing viral resistance was evaluated by passaging human coronavirus 229E (HCoV-229E) in the presence of increasing concentrations of rocaglates in MRC-5 cells. Importantly, no decrease in rocaglate-sensitivity was observed, suggesting that virus escape mutants are unlikely to emerge if the host factor eIF4A is targeted. In summary, all three rocaglates are promising antivirals with differences in cytotoxicity against human immune cells, RNA-clamping efficiency, and antiviral activity. In detail, zotatifin showed reduced RNA-clamping efficiency and antiviral activity compared to silvestrol and CR-1-31-B, but was less cytotoxic for immune cells. Our results underline the potential of rocaglates as broad-spectrum antivirals with no indications for the emergence of escape mutations in HCoV-229E.     

α-B crystallin在乳腺浸润性导管癌中的表达及意义

目的检测α-B crystallin在乳腺癌中的表达，并探讨其在乳腺癌中表达的意义。方法应用组织芯片及免疫组织化学法对180例乳腺浸润性导管癌、17例导管原位癌及20例正常乳腺组织中α-B crystallin以及雌激素受体（ER）、孕激素受体（PR）、HER2和Ki67的表达进行检测；根据最近提出的乳腺癌分子分型进行分组，对α-B crystallin在不同分组中的表达及与其他临床病理特征的关系行统计学分析。结果①α-B crystallin在乳腺导管原位癌的阳性率为41.17%（7/17），浸润性导管癌的阳性率为67.22%（121/180），两组间差异有统计学意义(P＜0.05）；②在浸润性导管癌中，α-B crystallin在淋巴结转移组阳性率为83.33%（81/96），无转移组阳性率为48.81%（41/84），两组间差异具有统计学意义（P＜0.01，r=0.368?8)；③在分子分型各组中α-B crystallin的表达差异具有统计学意义（P＜0.05）；其中LuminalA组与HER2+组及三阴性组两两比较差异具有统计学意义（P＜0.05）。结论α-B crystallin在乳腺浸润性导管癌中的表达明显高于导管原位癌，与淋巴结转移呈正相关，且在生物学行为更为恶性的HER2+组和三阴性组具有更高的表达水平，提示α-B crystallin蛋白可能参与了乳腺癌的浸润与转移过程。     

塞来昔布对子宫内膜癌细胞株HEC-1-B生长及其VEGF表达的影响

目的 研究塞来昔布对子宫内膜癌细胞株HEC-1-B的生长及其血管内皮生长因子(VEGF)表达的影响.方法 应用不同浓度的塞来昔布作用于HEC-1-B细胞,MTF法检测塞来昔布作用于HEC-1-B细胞24、48、72h后,对HEC-1-B细胞增殖的影响.用逆转录聚合酶链反应(RT-PCR)分析其对HEC-1-B细胞VEGF mRNA表达的影响,同时用酶联免疫吸附实验(ELISA)检测HEC-1-B细胞上清中VEGF蛋白表达量.结果 塞来昔布对HEC-1-B细胞的生长有抑制作用,且呈剂量和时间依赖性;与对照组相比,塞来昔布能降低HEC-1-B细胞VEGF mRNA及蛋白的表达.结论 塞来昔布能抑制HEC-1-B细胞的生长及VEGF的分泌.     

Regulation of antigen processing and presentation molecules in West Nile virus-infected human skin fibroblasts

Infection of humans with the West Nile flavivirus principally occurs via tick and mosquito bites. Here, we document the expression of antigen processing and presentation molecules in West Nile virus (WNV)-infected human skin fibroblast (HFF) cells. Using a new Flavivirus-specific antibody, 4G4, we have analyzed cell surface human leukocyte antigen (HLA) expression on virus-infected cells at a single cell level. Using this approach, we show that West Nile Virus infection alters surface HLA expression on both infected HFF and neighboring uninfected HFF cells. Interestingly, increased surface HLA evident on infected HFF cultures is almost entirely due to virus-induced interferon (IFN)alpha/beta because IFNalpha/beta-neutralizing antibodies completely prevent increased surface HLA expression. In contrast, RT-PCR analysis indicates that WNV infection results in increased mRNAs for HLA-A, -B, and -C genes, and HLA-associated molecules low molecular weight polypeptide-2 (LMP-2) and transporter associated with antigen presentation-1 (TAP-1), but induction of these mRNAs is not diminished in HFF cells cultured with IFNalpha/beta-neutralizing antibodies. Taken together, these data support the idea that that both cytokine-dependent and cytokine-independent mechanisms account for WNV-induced HLA expression in human skin fibroblasts.     

脐带血HLAⅠ类定型研究

分别采用血清方法和PCR-SSP基因定型方法对102份脐带血HLA-A,-B位点进行了检测,经对比分析结果显示血清学近18.6%不能得出满意结果,HLA-A,-B位点错定率分别在6.1%和10.8%,总错误率16.9%。我们设计了16条引物,以PCR-SSP方法特异性扩增B15等位基因,并与血清学做了比较,发现大多数B15裂解物的血清学检测结果是错误的。