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对5种透析膜及内毒素在体外对人单核细胞生成白细胞介素1(IL-1)的影响进行观察。结果表明,维持性血液透析患者的单核细胞已处于激活状态,接触透析膜和脂多醣后,IL-1的生成进一步增加,以醋酸纤维素膜最明显,脂多醣刺激单核细胞生成IL-1的作用更大,且醋酸纤维素膜和聚丙烯腈膜与脂多醣有协同刺激作用。 相似文献
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龙如章 《四川大学学报(医学版)》1985,(3)
对50例患慢性扁桃体炎因血清免疫球蛋白低而不宜进行扁桃体切除术的患儿,采用胎盘脂多醣治疗。治疗开始后2~6月观察临床疗效,94%患儿发病减少,症状减轻。远期随访47例,随访时间最长者3年零7月,痊愈好转率为91.5%;仅9例因并发症或治疗无效需行扁桃体切除术。免疫球蛋白的动态观察,近期IgG上升较明显,远期IgG和IgA增长显著。 相似文献
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用血清学检查法诊断伤寒、副伤寒国内推荐使用肥达试验微量凝集法[1],但微量凝集法存在阳性率不高,不能早期诊断等问题。笔者采用浙江省卫生防疫站研制的伤寒、副伤寒早期快速诊断试验,对伤寒、副伤寒病的诊断有早期、快速、准确的优点,现介绍如下:1测定原理将具有免疫学及紫外扫描纯度的伤寒、副伤寒沙门菌细胞壁的脂多而(LPS)致敏准化、醛化的“O”型人红细胞或绵羊红细胞,采用间接血凝(PHA)技术检测待检血清中相对应的抗体及抗体的滴度。2试验材料器材用120”V型微量反应板,稀释棒,微量移液器。试剂用浙江省卫生防疫站提… 相似文献
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对5种透析膜及内毒素在体外对人单核细胞生成白细胞介素1(IL-1)的影响进行观察。结果表明,维持性血液透析患者的单核细胞已处于激活状态,接触透析膜和脂多醣后,IL-1的生成进一步增加,以醋酸纤维素膜最明显,脂多醣刺激单核细胞生成IL-1的作用更大,且醋酸纤维素膜和聚丙烯腈膜与脂多醣有协同刺激作用。 相似文献
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HONG YU XIAO ZHU WEN QING ZHANG RUI Lü Department of Microbiology College of Medicine Qingdao University Qingdao P. R. China 《中华微生物学和免疫学杂志(英文版)》2006,4(2):146-152
To explore the antiviral effect and mechanism of polysaccharide from Spirulina platensis (PSP) on herpes simplex vims type 2 (HSV-2), a standard strain of HSV-2 (333 strain) was used to investigate the antiviral effect of PSP in vitro . PSP in various concentrations was applied to different stages of HSV-2 replication cycle. Finally, the virus infectivity (TCID50),cytopathic effect (CPE), and MTT staining method for viable cells (MTT assay) were used as markers to evaluate the effect of PSP on HSV-2. The quantity of HSV-DNA was detected by real-time fluorescence quantitative PCR (FQ-PCR). The HSV-2 infected Vero cell ultrastructures were observed by transmission electron microscopy (TEM). The results showed that PSP had little cytotoxic effect on Vero cells, it could not directly inactivate HSV-2 infectivity. PSP not only interfered in adsorption of HSV-2 to Vero cells but also inllibited HSV-2 biosynthesis in the cells. FQ-PCR results showed that the inhibitory rate on HSV-DNA also increased in a dose-dependent and time-dependent manner. TEM also confirmed that PSP exhibited pronounced inhibitory effect on HSV-2. In conclusion, the antiviral effect of PSP on HSV-2 may be attributed to the inhibition of vims adsorption, vims replication and synthesis in cells. 相似文献
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