介入性肺脏病学在早期非小细胞肺癌诊断及治疗中的应用

肺癌的治疗非常复杂,需要多学科联合来提供综合治疗。介入性肺脏病学是利用微创的方式为疑似肺癌的患者进行初步诊断和分期,在目前仍是一个不断发展的学科领域。支气管超声引导下的对纵隔淋巴结采样进行分期,同时进行驱动突变检测是早期和晚期肺癌诊断及治疗的重要工具,支气管超声引导下支气管镜的进步使得可疑周围性病变的组织学采样的并发症发生率大大降低同时为立体定向放疗植入基准标记。此外,介入性肺脏病学可以缓解不可手术的癌症及晚期癌症。由于低剂量CT扫描用于肺癌的早期发现,对肺部结节的治疗有经验的肺病专科医师来说,最重要的是尽可能减少侵袭性采样,整合多学科诊治的模式进行分期。     

MACC1基因干涉对食管癌细胞Eca109体外和体内增殖的影响

目的:探讨MACC1对食管癌细胞Eca109增殖的影响。方法:利用慢病毒载体介导的小发卡RNA(shRNA)干涉食管癌细胞Eca109中MACC1的表达。利用MTT法和平板克隆试验检测MACC1对食管癌细胞增殖的影响,利用流式细胞仪检测细胞周期的变化,最后利用裸鼠成瘤试验检测MACC1对食管癌细胞体内增值的影响。结果:成功建立MACC1干涉的食管癌细胞,MTT和平板克隆结果显示MACC1基因干涉后抑制食管癌细胞的增殖,并使食管癌细胞发生G1期阻滞。体内试验进一步证实MACC1干涉后抑制食管癌细胞在体内的增值。结论:干涉MACC1基因可抑制食管癌细胞的增殖。     

表达谱基因芯片筛选体外循环前后心肌细胞因子的变化

Objestive Systemic inflarmmation may be triggered by injury, hypothermia, ischemia-reperfusion and the contact of the blood with foreign body during cardiopulmonary bypass (CPB). To determine the application values of gene chip technique in the clinical practice and the study of cardiovascular stagery, as well as to provide clues to the study of inflammatory responess during CPB, microarry for gene expression profiles was used to identify the differences in the gene expression of myocardium between pre-and post- CPB. Methods Six adult patients who underwent CPB from March to May in 2003 were involved. Samples of right atrium were col- lected before and at immediate end of CPB. BD AtlasTM cDNA Expression Arrays was used to identify the differences in the gene ex- pression of cytokines. The results were compared with that of semi-quantative RT-PCR. Resellts The mean age of 6 patients (5 males and 1 female) was (32.67± 11.72) years. The baseline heart function was gradeⅡin 3 cases and grade Ⅲ in 3 other cases. The baseline left ventricular ejection fraction(LVEF)was (58.17±7.91)%. The mere duration was (91.67±43.88) minutes for CPB and was (58.67±43.46) minutes for aorta blocking. The minimum nasopharynx/rectal temperture was (29.37±1.90)℃/ (32.15±1.52)℃. Gene expression profiles of cytokines in the myocardium pre- and post-CPB were analysed successfully. The ex- pression of IL-6, IFN-γ,Wnt5a, TNFRSF1B, a member of tumor necrosis factor receptor superfamily, PIGF and MFNG in the myo- cardium were unpregulated after CPB. Conclusion Microarray technique is applicable in the study of cytokines changes dying CPB. cDNA microarray identified pleliminarily the differences in the gene expression between pre- and post-CPB. These genes may be in- valved in inflammation and other psthophysiological responses incuced by CPB. The myocardiym is probably one of the major sources of cytokines during CPB. Further study may be helpful in understanding the llngthe development of inflammation during CPB, and eventually, reducing the post-operative complications.     

中国汉坦病毒H82O5株G2糖蛋白基因的克隆及在真核细胞中的瞬时表达

从感染病毒乳鼠脑组织提取总RNA，采用RT－PCR和分子克隆技术将扩增到的G2糖蛋白基因插入含CMV启动子的pcDNA3.1/His质粒载体中，通过脂质体介导转染COS－7细胞，用SDS－PAGE、Western-blot及IFIA方法分别测定表达产物的相对分子量及特异性。结果证明获得正向插入的G2－pcDNA3.1/His重组表达质粒，表达产物的相对分子量为56ku，与理论预期大小一致，并且可与汉坦病毒H8205株的腹水抗体起特异反应。表明构建的G2－pcDNA3.1/His重组质粒所表达的蛋白为中国汉坦病毒株特有，能在哺乳动物细胞中表达并具有抗原性，重组质粒可应用于汉坦病毒的DNA疫苗研究。     

基因芯片技术在医学研究中的应用

基因芯片技术是同时将大量的探针分子固定到固相支持物上 ,借助核酸分子杂交配对的特性对DNA样品的序列信息进行高效的解读和分析。它可用于基因表达谱的分析、突变检测 ,克隆选择和文库筛选等研究工作 ,同时在人类疾病的检测 ,预防等方面也具有广阔的应用价值 ,在未来的生命科学领域中必将发挥重要作     

腰椎间盘内Ⅸ型胶原基因表达的观察

腰椎间盘内Ⅸ型胶原约占椎间盘胶原总量的 1 %～ 3 % [1 ] ,其确切的作用和分布还不清楚。我们以原位杂交的方法探讨胎儿、正常不同年龄的成人以及病理椎间盘中Ⅸ型胶原基因表达的规律。1 .资料与方法 :标本的制备 :(1 )胎儿组 :保胎失败胎龄 7个月的胎儿 ,在 4ｈ内无菌操作下取出椎间盘。 (2 )正常成人组 :取自意外死亡的成人 (2 2岁 )。(3)病变组 :取自椎间盘突出症患者的腰4 5和腰5 骶1 手术标本。将以上标本制备成厚度为 1 0 μｍ的原位杂交冰冻切片置于 - 70℃的低温冰箱中备用。含有Ⅸ型胶原ｃＤＮＡ探针序列的重组质粒ｐＭＣｏｌ1 0…