复方青风藤胶囊的主要药效学研究

目的：探讨复方青风藤胶囊（以下简称“QFC”）治疗类风湿性关节炎的主要药效作用。方法：观察QFC对大鼠佐剂性关节炎模型关节病变、免疫器官重量及血清TNF-α含量的影响，并研究其抗炎、镇痛作用。结果：与模型对照组相比，QFC能明显减轻佐剂性关节炎模型（AA）大鼠的关节病变及降低肿瘤坏死因子（TNF-α）水平与胸腺指数；能显著抑制乙酸致小鼠的腹膜毛细血管通透性；显著降低角叉菜胶致炎大鼠的足跖肿胀率；能明显减少乙酸致小鼠的扭体次数；QFC能显著提高热致痛小鼠的痛阈值。结论：QFC可以对抗类风湿性关节炎这类免疫异常引发的疾病。其作用机理可能与其免疫抑制作用与较好的抗炎镇痛作用有关。     

Juvenile Rheumatoid Arthritis—Madras experience

In a prospective study of 1,053 consecutive children who attended the Rheumatic Care Centre, Government General Hospital, Madras from 1991 to 1995, 331 children fulfilled the criteria proposed by the American Rheumatism Assocation as modified by Cassidyet al for the diagnosis of Juvenile Rheumatoid Arthritis. These children were thoroughly examined and investigated and classified into 3 onset types which was then sub-classified into early entry and late entry groups based on the duration of illness. Other arthritic conditions were excluded. There were 44 cases belonging to Systemic onset, 171 belonging to polyarticular onset and 116 belonging to oligoarticular onset type. In the systemic onset type 44/44 patients had fever, 40/44 had lymphadenopathy and 19/44 had skin rash; wrists and knees 31/44 were the most commonly involved joints; neck involvement was present in 13/44 of the cases; ANA was positive in 5/44 cases and anaemia was seen in 24/44 cases. In polyarticular onset type wrists 119/171, knees 143/171, hip joints 105/171 and ankles 113/171 were commonly involved; in the RF+ve subtype 3/23 had subcutaneous nodules and 7/23 were positive for ANA; in the Rf-ve subtype 59/148 were positive for ANA. In the oligoarticular subtype-1 ANA was positive in all cases but iridocyclitis was not seen in any case. In oligoarticular subtype-2 HLA B27 was positive in 13/26 cases while Sacroilitis was seen in 16/26 cases. In oligoarticular type-3 HLA B27 was negative.     

幼年特发性关节炎患儿免疫学指标检测的意义

目的探讨幼年特发性关节炎(JIA)患儿体液和细胞免疫部分指的标变化及其临床意义。方法检测218例JIA患儿的免疫球蛋白(Ig)IgG、IgAI、gM,补体C3、C4,类风湿因子(RF),抗核抗体(ANA),抗双链DNA抗体(ds-DNA)及分化群(CD)CD3 、CD4 、CD8 及CD4 /CD8 值;对各亚型JIA与正常对照组CD3 、CD4 、CD8 、CD4 /CD8 测定值进行比较。结果各亚型JIA近半数患儿IgG、IgAI、gM及C3升高,RF阳性率5.2%,ANA滴度升高率11.1%,与附着点相关的关节炎(ERA)和少关节型人类白细胞抗原(HLA)-B27阳性率分别达100%、20%。各亚型JIA CD4 、CD8 水平与正常对照组比较有显著性差异,CD4 /CD8 值无显著性差异。结论JIA患儿存在明显的体液及细胞免疫功能紊乱。     

CyclinB1和CyclinD3基因在类风湿关节炎滑膜组织中的表达

滑膜衬里层增厚是类风湿关节炎的重要病理学特征之一，但机制不清。本研究用核酸分子原位杂交技术检测反映细胞增殖状况的细胞周期蛋白基因ＣｙｃｌｉｎＢ１和ＣｙｃｌｉｎＤ３在１０例ＲＡ、３例骨关节炎及４例正常关节滑膜组织的ｍＲＮＡ表达水平。发现ＣｙｃｌｉｎＢ１ｔ ＣｙｃｌｉｎＤ３在ＲＡ滑膜衬里层表达明显高于对照组，提示滑膜细胞的原位增殖在ＲＡ滑膜衬里层增厚中可能起一定作用。