Hormonal therapy in undescended testes

A clinical study trial of HCG therapy in 78 selected prepubertal cryptorchids (40 unilateral and 38 bilateral) haveing a total of 116 undescended gonads revealed that complete descent occurred in 12.5% gonads (13% in unilateral and 12% in bilateral group). Good response was observed in only those gonads which were located in the inguinal canal and at the external ring, irrespective of it being right or left sided gonad. In patients with impalpable testes, though there was no complete descent, yet partial descent was noted in 18% (21/116 gonads). The response was better in the older children.     

DEHP诱导的隐睾鼠胰岛素样因子3基因CpG岛甲基化状态研究

目的 研究胰岛素样因子3(insulin-like factor 3,INSL3)基因在邻苯二甲酸二已酯(DEHP)致小鼠隐睾中DNA甲基化的状态.方法 运用"MethPrimer"软件对INSL3基因进行分析.预测CpG岛,通过甲基化特异性PCR(methylation-specific PCR,MS-PCR)检测INSL3基因CpG岛的甲基化状况,并应用RT-PCR检测INSL3基因在正常组与实验组睾丸组织中的表达情况.结果实验组INSL3基凶第一外显子区存在CpG岛甲基化;实验组INSL3基因mRNA表达显著下降.结论 INSL3基因第一外显子区CpG岛的异常甲基化导致INSL3在隐睾鼠睾丸中表达降低,可能与隐睾的发生密切相关.     

Immunolocalization of Epidermal Growth Factor in Human Fetal, Adult and Cryptorchid Testes and Seminoma

EGF was localized in human fetal, adult and eryptorchid testis and seminoma using an immunohistoehemical method. Leydig cells of adult testes stained intensely for EGF while those of fetal testes showed weak staining reaction. In addition, some spermatogonia and occasional peritubular myoid cells of adult testis stained positively. In cryptorchid testis, there were clusters of Leydig cells interspersed between seminiferous tubules with varying staining intensities. The number of positiuely stained cells in the cryptorchid testis were fewer than that in adult testes. The decrease in the number of Leydig cells pruducing EGF may account for the spermatogenesis arrest and infertility, associated with this disorder.Intense staining of the cytoplasm of seminoma cells was obserued, suggesting that the high production of EGF may be related to their invasive property. In conclusion, EGF is produced by human testis and Leydig cells are the principal source of this cytokine.