淋巴因子水平改变与妊高征的关系

近年来的研究结果发现由Th1/Th2细胞分泌的淋巴因子与妊娠高血压综合征联系密切 ,可能参与了其病因及病理生理过程 ,对它的深入研究有利于妊娠高血压综合征诊断、治疗水平的提高     

Phenotypic and functional properties of murine {gamma}{delta} T cell clones derived from malaria immunized, {alpha}{beta} T cell-deficient mice

Six murine T cell clones expressing TCR were generated frommalaria immunized, ß T celldeficient mice. Phenotypiccharacterization of these clones has revealed that, in contrastto conventional ß T cells, there is a considerabledegree of heterogeneity among these clones with regard to theirsurface markers and their lymphokine profile. One clone wasfound to display significant anti-parasite activity in vivoupon adoptive transfer. We attempted to determine whether theprotective clone differs in one or more key characteristicsfrom the non-protective clones. Although no obvious patternpeculiar to the protective clone was observed, it appears thatmore than one parameter may, in combination, define a distinctprotective phenotype, and thus explain the functional differencebetween the protective and non-protective clones.     

Characterization of the murine interleukin 5 receptor by using a monoclonal antibody

Murine interleukin 5 (IL-5), a lymphokine produced by helper T cells, is involved in the regulation of growth and differentiation of B cells and other hematopoietic cells. The receptor for IL-5 has been identified as two cross-linked complexes on T88-M cells (a murine IL-5-dependent early B cell line). In this study the IL-5 receptor was directly characterized by utilizing an immobilized IL-5 column and a rat monoclonal antibody, designated H7, directed against the IL-5 receptor. H7 completely inhibited specific binding of 35S-labeled IL-5 to T88-M cells, and bound to IL-5-responsive cells, e.g. T88-M, BCL1-B20 (a chronic B-cell leukemia), and MOPC104E (a myeloma), whereas H7 did not bind to IL-5-non-responsive cells, e.g. X5563 (a myeloma), FDC-P1 (an IL-3-dependent line), and MTH (an IL-2-dependent CTLL). H7 could barely bind to T88-M cells in the presence of IL-5, and immunoprecipitated a major band with an Mr of approximately 60 kd from the extract of surface-radioiodinated T88-M cells. The precipitation of this 60 kd molecule was inhibited by the addition of IL-5. Analysis with immobilized IL-5 also revealed that a 60 kd molecule bound specifically to IL-5-coupled beads compared with control beads. Furthermore, no additional molecule with a higher Mr that was recognized by H7 appeared under non-reducing, compared with reducing, conditions. The 60 kd molecule recognized by H7 could be digested with N-glycanase to yield a protein band of approximately 55 kd.(ABSTRACT TRUNCATED AT 250 WORDS)     

抗肿瘤多价转移因子对肿瘤患者NK及LAK活性的影响

目的：制备并观察抗肿瘤多价转移因子（M－TF）对肿瘤患者NK及LAK活性的影响。方法：分别用肝癌细胞悬液,胃癌细胞悬液免疫山羊,取其淋巴组织均匀浆透析法制备肝癌特异性转移因子（HCC－STF）和胃癌特异性转移因子（GC－STF）,用肝癌,胃癌及大肠癌混合细胞悬液免疫山羊,制备M－TF。比较其理化性质及对肿瘤患者NK及LAK活性的影响。结果：三种转移因子理化性质十分相似,肽类,核苷酸类物质及氨基酸俯含量相近。HCC－STF能明显增加肝癌患者NK,LAK活性及LAK细胞对肝癌7721细胞的特异细胞毒作用,GC－STF能明显增加胃癌和大肠癌患者NK,LAK活性及LAK细胞对胃癌7901细胞的特异细胞毒作用,M－TF能显著增加肝癌,胃癌及大肠癌的NK,LAK活性及LAK细胞对7721和7901细胞的细胞毒作用,其增加效果与HCC－STF及GC－STF比无明显差异。结论：M－TF具有免疫调节性强,抗肿瘤谱广的特性,值得推广应用。     

Evidence for a Low-Affinity Interleukin-3 Receptor

Abstract

Interleukin-3 (IL-3) regulates the proliferation of myeloid, erythroid, and lymphoid cells. Previous reports showed IL-3 binding restricted to a single high-affinity (K_d = 50-200 pin) site. Here, we demonstrate by equilibrium studies an additional binding site for IL-3 with lower apparent affinity (K_d=5-20 iym). Furthermore, kinetic analysis shows that two binding sites for IL-3 exist: IL-3 dissociates slowly from the first site (I_½=4hr; k_?1=2.7x10^?3 min^?1), whereas it dissociates rapidly (T_½=4.0 min; k_?1 = 0.116 min^?1) from the second site. Cross-linking showed that [¹²⁵I]IL-3 binding to the 115- and 140-kD proteins was not saturable at concentrations commensurate with high-affinity binding and IL-3 dissociated rapidly from these same molecules. Thus, the low affinity IL-3 receptor is a molecule(s) of 115- to 140-kD.     

Histological changes of the liver in experimental graft-versus-host disease across minor histocompatibility barriers. VIII. Role of eosinophil infiltration

Abstract: Although eosinophil infiltrate has been recognized in hepatic graft-versus-host disease, its significance in relation to hepatic graft-versus-host disease lesions is unknown. In the present study, we analyzed hepatic eosinophil infiltration in relation to bile duct damage in experimental mouse graft-versus-host disease across minor histocompatibility barriers up to 14 months after transplantation. Portal eosinophil infiltration was found from 1 week after transplantation throughout the entire 14-month observation period. It was most striking during the early chronic stage of hepatic graft-versus-host disease between 2 to 7 months, with a peak at 5 months after transplantation. Microscopic and electron microscopic study revealed eosinophils infiltrated around the bile duct as well as in the bile duct epithelial layer. They were commonly found together with lymphocytes but were also occasionally found singly around the bile duct and in the bile duct epithelial layer. Bile duct epithelial cells in contact with and in the vicinity of eosinophils showed a variety of degenerative changes, occasionally associated with the presence of extracellular eosinophil granules. Bile duct epithelial cells with eosinophil infiltration just beneath the basement membrane frequently showed further characteristic severe degenerative changes with shedding or dropping-off into the lumen, which features were quite similar to those seen in the bronchial epithelium in asthma patients. These results indicate that not only lymphocytes but also eosinophils may be involved in the production of the bile duct injury in hepatic graft-versus-host disease, especially in its early chronic stage.     

Clinical Study on General lmmunotherapy of Hepatocellular Carcinoma

In the present study,67 cases of hepatocellular carcinoma(HCC)were treated withimmunotherapy or immunochernotherapy.The results indicated that natural killer cell(NK)andantibody-dependent cytotoxicity cell(ADCC)activities and lymphocyte transformation rate(LTR)were significantly improved in vivo after treatment.The NK activity in tumor tissue,which showed aheavy immunosuppression,could also be enhanced and recovered to normal level after treatment withlymphokine mixture and 5-fluorouracil(5-FU).Immunity of host with postoperativeimmunochemotherapy was rapidly improved and recovery vate was high.The survival time in thesecases of unresectable tumors was obviously prolonged when a general immunotherapy was used.Immunochemotherapy can impr(?)ve immunity and inhibit selectively Ts cells as well as kill directly thetremor cells;therefore,it can play an important role in the treatment of hepatocellular carcinomaand in the prevention of its recurrence after operation.     

CD3AK细胞和LAK细胞治疗晚期恶性肿瘤的临床和实验研究

将５１例晚期恶性肿瘤患者（男性２３例，女性２８例）分成两组，其中一组（３１例）以ＣＤ３ＭｃＡｂ（ＣＤ３单克隆抗体）和小剂量ＩＬ－２（５００ｕ／ｍｌ）共同诱导的ＣＤ３ＡＫ细胞治疗，另一组（２０例）输注大剂量ＩＬ－２（１０００ｕ／ｍｌ）诱导的常规ＬＡＫ细胞治疗，以探讨降低ＩＬ－２用量、提高杀伤细胞细胞毒活性的可能性。结果显示ＣＤ３ＡＫ组患者生活质量改善、症状缓解均优于ＬＡＫ组。ＣＤ３ＡＫ组ＰＲ＋ＭＲ率较ＬＡＫ组高２９．０％，Ｓ＋Ｐ率和死亡率分别较ＬＡＫ组低１２．４％和９．６％。同时比较了ＣＤ３ＡＫ细胞与ＬＡＫ细胞的体外增殖和细胞毒活性，结果表明ＣＤ３ＡＫ细胞增殖率高于ＬＡＫ细胞（Ｐ＜０．０１），靶细胞抑制率二者在０．０５水平无显著差异。提示ＣＤ３ＭｃＡｂ在刺激杀伤细胞活性，尤其在提高其增殖能力方面，具有显著的作用，ＣＤ３ＡＫ／ＩＬ－２能更有效地治疗晚期恶性肿瘤。     

杂环化合物九钨三钛硅酸盐对小鼠免疫功能的影响

目的 :探讨杂环化合物九钨三钛硅酸盐 ( α- Ti3 )对小鼠细胞免疫和体液免疫功能的影响。方法 :采用3 H- Td R掺入法测定正常鼠和荷瘤鼠脾细胞对有丝分裂原的增殖反应及 NK和 LAK细胞活性。采用 T淋巴母细胞增殖分析法测定荷瘤鼠脾细胞 IL- 2的产生及其活性。采用单向琼脂扩散法测定血清 Ig G和补体 3含量。结果 :α- Ti3 的 5 0、1 0 0和 2 0 0 mg·kg-1剂量组脾细胞对有丝分裂原的增殖反应、NK和 LAK细胞活性以及脾细胞 IL- 2产生反应性及活性明显高于正常对照组和环磷酰胺 ( CYC)组 ( P<0 .0 5 ) ,而 1 0 0 mg· kg-1 组的作用尤为明显。结论 :α- Ti3 可增强荷瘤鼠的细胞免疫和体液免疫功能     

Modulating effect of mitomycin or cisplatin on lymphokine-activated killer cell proliferation and antitumor activity to bladder cancer cell lines in vitro

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