Comparative Study on the Immunogenicity between Recombinant MS-Sj26GST Vaccine and Recombinant BCG-Sj26GST Vaccine in Schistosoma japonicum

TuberculosisandSchistosomiasisarethemajorcontagiousdiseaseswhicharethemostdangeroustothepeople’shealth Inordertogetridofthem ,wemustlookforamoreusefulvaccine Bythetech niquesofmolecularbiology ,2 6 0 0 0DaGlutathionStransferase (GST) genewasclonedintotheE coli MycobacteriumtransferringandexpressionvectorpBCG 2 0 0 0totransformittoMycobacteriumsmeg matismc2 15 5 (MS)andBCGseparatelyinordertoconstructrMS Sj2 6GSTvaccineandrBCG Sj2 6GSTvaccine Inthisstudy ,theBALB/cmicewereimmu niz…     

双特异性抗体对LAK细胞增殖和细胞毒作用影响的体外研究

将抗ＣＤ３与抗ＨＢｓ的单克隆抗体经化学偶联得到双特异性抗体，观察该双特异性抗体对ＬＡＫ细胞增殖和增强细胞毒性的作用。结果显示双特异性抗体显著提高ＬＡＫ细胞与２．２．１５细胞结合率；促进淋巴细胞增殖。１２５Ｉ－ＵｄＲ释放试验检测发现双特异性抗体增强ＬＡＫ细胞对２．２．１５细胞的细胞毒性且与抗体浓度呈正相关。进一步对抗体的特异性研究表明，加入双特异性抗体后ＬＡＫ细胞对２．２．１５细胞毒性作用显著高于对照组。     

The construction ofSchistosoma Japonicum vaccine BCG-Sj26GST and its identification

Summary The expression of foreign gene,Schistosoma Japonicum 26 ku antigen (Sj26GST),in Bacillus Calmette-Guerin (BCG),Mycobacterium CM. smegmatis) andEscherichia coli (E. coli) were studied. The cDNA fragment encoding Sj26GST was amplified by PCR using plasmid pGEX, which could express Sj26GST inE. coli as template. The Sj26GST cDNA was cloned into the downstream of humanM. tuberculosis heat shock protein (hsp) 70 promoter with correct reading frame, and then the DNA fragment containing hsp70 promoter and Sj26GST gene were subcloned together intoE. coli-Mycobacteria shuttle plasmid pBCG-2000 to construct the expression shuttle plasmid pBCG-Sj26. The recombinant BCG andM. smegmatis mc²155, which were electroplated with pBCG-Sj26, could express Sj26GST and the recombinantSchistosoma Japonicum vaccine BCG-Sj26GST was made. The recombinant Sj26GST (rSj26GST) were soluble and could be observed on SDS-PAGE at molecular weight of 26 ku. The content of rSj26GST accounted for 15% and 10% of total bacterial protein in BCG andM. smegmatis respectively. The results of Western blot showed the combination of rSj26GST with antibody of GST. This project was supported by Primary Foundation of China (No. 94-Y-19), Chinese National Nature Science Foundation (No. 339480022) and Chinese Health Foundation (No. 94-1-131).     

In vitro and in vivo antifungal activities of the eight steroid saponins from Tribulus terrestris L. with potent activity against fluconazole-resistant fungal pathogens

Antifungal activity of natural products is being studied widely. Saponins are known to be antifungal and antibacterial. We have isolated eight steroid saponins from Tribulus terrestris L. , namely TTS-8, TTS-9, TTS-10, TTS-11, TTS-12, TTS-13, TTS-14 and TTS-15. TTS-12 and TTS-15 were identified as tigogenin-3-O-β-D-xylopyranosyl（1→ 2）-[-β-D-xylopyranosyl（ 1 → 3 ） 3-β- D-glucopyranosyl （ 1 → 4 ）- 1- α-L-rhamnopyranosyl （ 1 → 2 ） 3-β-D-galactopyranoside and tigogenin-3-O-β-D-glucopyranpyranosyl（1→2）-[-β-D-xylopyranosyl（1→ 3）3-β-D-glucopyranosyl（1→4）-β-D-galactopyranoside, respectively. The in vitro antifungal activities of the eight saponins against six fluconazole-resistant yeasts, Candida albicans, Candida glabrata, Candida para psilosis , Candida tropicalis , Candida krusei , and Cryptococcus neo f ormans were studied using microbroth dilution assay. The results showed that TTS-12 and TTS-15 were very effective against several pathogenic candidal species and C. neoformans in vitro. It is noteworthy that TTS-12 and TTS-15 were very active against fluconazole-resistant C. albicans （MIC80 = 4.4, 9.4 mg/ml）, C. neoformans （MIC80 =10.7, 18.7 mg/ml） and inherently resistant C. krusei （MIC80 =8.8, 18.4 mg/ml）. So in vivo activity of TTS-12 in a vaginal infection model with fluconazole-resistant C. albicans was studied in particular. Our studies revealed TTS-12 also showed in vivo activities against fluconazole-resistant yeasts. In conclusion, steroid saponins TTS-12 and TTS-15 from Tribulus terrestris L. have significant in vitro antifungal activity against fluconazole-resistant fungi, especially TTS-12 also showed in vivo activity against fluconazole-resistant C. albicans.     

肝癌患者中医辨证分型与肝癌细胞体外化疗药物敏感性的关系

目的:观察不同中医辨证分型肝癌患者肝癌细胞体外化疗药物敏感性.方法:选取60例肝癌切除标本,利用MTT法测定不同证型肝癌患者对6种常用化疗药物的体外敏感性.结果:脾气虚组、肝气郁结组、瘀血阻络组之间各药敏感性有逐步下降趋势,但无统计学意义;各药在肝瘀脾虚阻络组敏感性明显下降.结论:不同中医辨证分型肝癌患者对化疗药物的敏感性存在一定差异,中西医结合个体化治疗肝癌,就是要根据具体患者药敏实验结果结合辨证论治设计治疗方案.     

腓骨长肌腱前半部作为自体移植材料的临床研究

 目的 探讨腓骨长肌腱前半部（anterior half of the peroneus longus tendon,AHPLT）作为自体肌腱移植材料重建膝关节韧带的可行性及疗效。方法 2007年7月至2008年1月采用AHPLT作为自体肌腱移植材料的膝关节韧带损伤患者100例,男33例,女67例;年龄16~62岁,平均32.3岁。关节镜下内侧髌股韧带重建49例、多条韧带重建19例、后十字韧带双束重建18例和前十字韧带双束重建14例。切取AHPLT作为全部（49例）或部分（51例）重建材料,采用单切口或双切口技术,重建韧带用螺钉挤压固定。术后评估膝关节Kujala评分、Lysholm评分、Marx评分、国际膝关节文献委员会（International Knee Documentation Committee,IKDC）膝关节主观评估表和客观等级评定、踝关节足踝功能障碍指数（Foot and Ankle Disability Index,FADI）及美国足踝外科学会（American Orthopedic Foot and Ankle Society,AOFAS）评分。结果 92例获得2年以上随访。术后2年,不同韧带重建组患者膝关节IKDC主观评分、Kujala评分、Lysholm评分及Marx评分均高于重建术前。多条韧带重建、后十字韧带双束重建和前十字韧带双束重建术后IKDC客观等级评定结果达到正常及接近正常者分别为17例、15例和12例,优良率分别为89.5%（17/19）、93.7%（15/16）和100%（12/12）。全部患者手术前后AOFAS评分分别为（97.4±2.0）分和（97.2±1.6）分,FADI评分分别为（96.8±2.2）分和（96.9±2.5）分,差异均无统计学意义。患者均未出现腓神经损伤、腓骨长肌腱断裂等并发症。结论 AHPLT作为自体肌腱移植材料重建膝关节韧带具有操作可行性,近期临床疗效好,切取肌腱后对踝关节功能影响小。     

Novel Oxazolidinone Antibacterial Analogues with a Substituted Ligustrazine C‐ring Unit

A series of novel oxazolidinone compounds with a substituted ligustrazine C‐ring unit and different substituted groups at the C‐5 side chain were designed and synthesized using linezolid as a lead and based on a scaffold hopping strategy. Their antibacterial and anti‐inflammatory activities were evaluated. The results of in vitro antibacterial assays showed that all fourteen target compounds displayed potent activity against Gram‐positive pathogens, particularly 8b , 13b , 14a , 14b , 15a, and 15b . Moreover, 14a and 14b exhibited significant inhibitory activities on the production of inflammatory mediators, including nitric oxide, interleukin‐6, and tumor necrosis factor‐alpha. Thus, these derivatives could serve as valuable candidates to develop anti‐infective agents for the treatment of chronic wounds.     

Computational study on natural compounds inhibitor of c-Myc

To screen and identify ideal leading compounds from a drug library (ZINC15 database) with potential inhibition effect against c-Myc to contribute to medication design and development.A series of computer-aided virtual screening techniques were performed to identify potential inhibitors of c-Myc. LibDock from the software Discovery Studio was used to do a structure-based screening after ADME (absorption, distribution, metabolism, excretion) and toxicity prediction. Molecular docking was utilized to show the binding affinity and potential mechanism between ligands and c-Myc. Stability of the ligand-receptor complex was analyzed by molecular dynamic simulation at the end of the research.Compounds with more interactive energy which are confirmed to be the potential inhibitors for c-Myc were identified from the ZINC15 databases. Additionally, those compounds are also anticipated with fewer ames mutagenicity, rodent carcinogenicity, nondevelopmental toxic potential, and tolerant with cytochrome p450 2D6(CYP2D6). Dynamic simulation analysis also revealed that the very compounds had more favorable potential energy compared with 10058-F4(ZINC12406714). Furthermore, we prove that those compounds are stable and can exist in natural conditions.This study demonstrates that the compounds are potential therapeutic inhibitors for c-Myc. These compounds are safe and stable for drug candidates and may play a critical role in c-Myc inhibitor development.     

硬膜下积液治疗经验总结（附27例临床分析）

目的总结不同类型硬膜下积液治疗经验。 方法对解放军总医院第六医学中心神经外科自2009年1月至2014年10月手术治疗并完整随访的27例硬膜下积液患者进行回顾性分析。根据术前影像学特征鉴别积液是否为血性,将患者分为血性硬膜下积液患者（9例）和非血性硬膜下积液患者（18例）。根据积液是否为血性选择个性化治疗方案,观察其疗法。 结果9例血性硬膜下积液患者接受钻孔外引流手术,8例积液消退,另外1例无效,之后接受硬膜下腹腔分流后治愈。非血性硬膜下积液患者中14例接受硬膜下腹腔分流,12例有效,2例术后出现脑积水,经脑室-腹腔分流术治愈;2例术前合并脑积水接受脑室-腹腔分流术,均有效;另外2例最初接受积液外引流,无效,之后行硬膜下腹腔分流后积液消退。 结论对于硬膜下积液患者,术前需仔细评估积液是否为血性,是否合并脑积水。血性积液采取钻孔外引流,非血性积液采取硬膜下腹腔分流,合并脑积水的积液采取脑室-腹腔分流手术方式,给予个体化治疗,可获得满意疗效。     

养心汤对同型半胱氨酸致大鼠血管内皮功能障碍的影响

目的通过高蛋氨酸饲料喂养SD大鼠建立血管内皮功能障碍模型,观察中药养心汤对血管内皮功能障碍的影响并对其相关机制进行探讨。方法 SD雄性大鼠40只,按随机数字表法分为空白组、模型组、叶酸组、养心汤组,每组10只。空白组喂养常规普通饲料,模型组、叶酸组、养心汤组喂养含3%L-蛋氨酸的常规普通饲料,共12周,建立血管功能障碍模型。模型建立成功后,空白组和模型组大鼠灌胃等体积纯净水;叶酸组大鼠灌胃叶酸片混悬剂1.8 mg/(kg·d),养心汤组大鼠灌胃养心汤浸膏混悬剂2.34 g/(kg·d),灌胃4周。实验结束后,大鼠麻醉称重取材,取胸主动脉电镜观察血管内皮超微结构变化;腹主动脉取血离心,ELISA法检测血清一氧化氮(NO)、内皮素-1(ET-1)、同型半胱氨酸(Hcy)、蛋白C受体(sEPCR)、可溶性血栓调节蛋白(s TM)水平。结果电镜下可见空白组大鼠血管内皮完整连续未见明显损伤,与空白组比较,模型组大鼠血管内皮大部分脱落,偶见残存内皮,损伤程度明显加重;与模型组比较,养心汤组大鼠血管内皮基本连续完好,损伤程度明显减轻;ELISA检测结果可见,与空白组比较,模型组大鼠血清NO水平降低、...