全文获取类型
收费全文 | 116195篇 |
免费 | 12146篇 |
国内免费 | 7741篇 |
专业分类
耳鼻咽喉 | 1157篇 |
儿科学 | 1318篇 |
妇产科学 | 910篇 |
基础医学 | 12316篇 |
口腔科学 | 2371篇 |
临床医学 | 14549篇 |
内科学 | 16003篇 |
皮肤病学 | 1341篇 |
神经病学 | 5534篇 |
特种医学 | 4876篇 |
外国民族医学 | 46篇 |
外科学 | 12115篇 |
综合类 | 22506篇 |
现状与发展 | 34篇 |
一般理论 | 13篇 |
预防医学 | 8896篇 |
眼科学 | 2816篇 |
药学 | 12757篇 |
155篇 | |
中国医学 | 7550篇 |
肿瘤学 | 8819篇 |
出版年
2024年 | 449篇 |
2023年 | 1801篇 |
2022年 | 4778篇 |
2021年 | 6225篇 |
2020年 | 4933篇 |
2019年 | 3853篇 |
2018年 | 3927篇 |
2017年 | 3984篇 |
2016年 | 3845篇 |
2015年 | 5801篇 |
2014年 | 7013篇 |
2013年 | 7033篇 |
2012年 | 10290篇 |
2011年 | 10882篇 |
2010年 | 7967篇 |
2009年 | 6412篇 |
2008年 | 7391篇 |
2007年 | 6840篇 |
2006年 | 6124篇 |
2005年 | 5435篇 |
2004年 | 3748篇 |
2003年 | 3508篇 |
2002年 | 2835篇 |
2001年 | 2113篇 |
2000年 | 1763篇 |
1999年 | 1512篇 |
1998年 | 873篇 |
1997年 | 830篇 |
1996年 | 597篇 |
1995年 | 623篇 |
1994年 | 450篇 |
1993年 | 298篇 |
1992年 | 366篇 |
1991年 | 273篇 |
1990年 | 248篇 |
1989年 | 230篇 |
1988年 | 162篇 |
1987年 | 165篇 |
1986年 | 153篇 |
1985年 | 86篇 |
1984年 | 47篇 |
1983年 | 29篇 |
1982年 | 30篇 |
1981年 | 22篇 |
1980年 | 19篇 |
1979年 | 20篇 |
1974年 | 10篇 |
1973年 | 14篇 |
1972年 | 9篇 |
1969年 | 12篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
Bortezomib is a novel proteasome inhibitor, which has been successfully used to treat mantle cell lymphoma and multiple myeloma. However, the direct effects of bortezomib on acute promyelocytic leukaemia (APL) have not been fully investigated. In the present study, the WST-8 assay, western blotting, flow cytometry, monodansylcadaverine staining and transmission electron microscopy were performed. It was demonstrated that bortezomib treatment induced a time- and dose-dependent decrease in the viability of NB4 cells. Bortezomib treatment induced cell apoptosis in NB4 cells, as assessed by Annexin V/propidium iodide analysis, and the detection of cleaved caspase-3, cleaved poly(ADP-ribose) polymerase, Bax and Bcl-2 expression. Furthermore, bortezomib treatment induced autophagy in NB4 cells, as indicated by autophagosome formation, p62 degradation, LC3-I to LC3-II conversion and formation of acidic autophagic vacuoles. Notably, autophagy induced by bortezomib was initiated prior to apoptosis. Inhibition of autophagy by knocking down Beclin-1 expression increased bortezomib-induced apoptosis in NB4 cells. Therefore, the present study revealed that the combination of bortezomib and autophagy inhibition may be a potential treatment strategy for APL. 相似文献
4.
Lei Yang You-Ling Shi Yan Ma Wei-Wei Ren Guang-Ming Pang Jiao Liu 《APMIS : acta pathologica, microbiologica, et immunologica Scandinavica》2022,130(1):43-52
Krüppel-like factor 16 (KLF16), a member of the Krüppel-like factor (KLF) family, has been extensively investigated in multiple cancer types. However, the role of KLF16 in oral squamous cell carcinoma (OSCC) remains unknown. Thus, we conducted this study to investigate its related mechanism. KLF16 expression in OSCC cell lines was quantified by western blotting. Then, OECM1 and OC3 cells were divided into Blank, siCtrl, siKLF16#1 and siKLF16#2 groups. Subsequently, cell proliferation was detected using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assays, cell migration and invasion were detected with wound healing and Transwell assays, and cell cycle distribution and cell apoptosis were detected via flow cytometry. KLF16, p21, CDK4, Cyclin D1 and p-Rb expression was detected by western blotting. Finally, xenograft models were established in nude mice to observe the in vivo effects of KLF16 on OSCC. KLF16 protein expression was upregulated in OSCC cells. Compared to the cells in the Blank group, the OECM1 and OC3 cells in the siKLF16#1 group and siKLF16#2 group exhibited a sharp decrease in proliferation but a remarkable increase in apoptosis. Moreover, the proportion of cells in the G0/G1 phase notably increased and that in the S phase decreased, with evident decreases in cell invasion and migration. Moreover, KLF16, cyclin-dependent kinase 4 (CDK4), Cyclin D1 and p-Rb protein expression was upregulated, but p21 expression was downregulated. The mice in the siKLF16#1 and siKLF16#2 xenograft model groups exhibited slower tumour growth and smaller tumours with evident downregulation of Ki67 expression compared to the mice in the Blank group. KLF16 expression was upregulated in OSCC cells, and interfering with KLF16 led to cell cycle arrest, inhibited OSCC cell growth and promoted cell apoptosis. 相似文献
5.
6.
Feng Wang Dachuan Wang Bingyi Tan Jun Dong Rongjie Feng Zenong Yuan Naiguo Wang 《Medicine》2015,94(39)
The present study aimed at examining the curative effect of modified posterior operation on treatment of Kümmell''s disease.About 30 patients of Kümmell''s disease with complete image and clinical data treated during June 2004 to July 2013 were conducted with anterior and posterior approaches, respectively. Kyphotic Cobb angle, fractured vertebra wedge angle, and the anterior and posterior heights of fractured vertebra were all measured through x-ray before and after operation, and the pain visual analog scale (VAS) was determined for evaluating the effect of operations. The injury and restoration of neurological function were assessed using Frankel classification.Patients in group A were treated with anterior operation, whereas group B was posterior operation. Postoperatively, VAS score, kyphotic Cobb angle, anterior vertebra height, and pathologic vertebra wedge angle were all significantly improved in patients with Kümmell''s disease receiving modified posterior operation (group B). Similar results were also observed in patients with anterior operation. The improvement of VAS and correction rate of kyphotic Cobb angle indicated equivalent effects of posterior and anterior operations. Meanwhile, alleviated neurological function damage was observed in 2 groups. Relevant factor analysis illustrated that there was no significant correlation of the severity and improvement rate of pain symptoms with age, medical history, anterior and posterior vertebra heights, kyphotic Cobb angle, and pathological vertebra wedge angle.Compared with traditional anterior approach, modified posterior operation, adopting transpedicular vertebral body grafting combined with vertebral pedicle screw fixation, could produce equivalent effects on kyphosis correction, pain relief, and improvement of neurological function in patients with Kümmell''s disease. 相似文献
7.
目的探讨脑卒中患者急性应激障碍发生现状及影响因素。方法采用斯坦福急性应激反应问卷对349例脑卒中住院患者进行调查。结果共163例(46.70%)患者发生急性应激障碍;Logistic回归分析结果显示,患者性格、是否存在偏瘫及是否吞咽功能障碍是脑卒中患者发生急性应激障碍的主要影响因素(P0.05,P0.01)。结论脑卒中患者急性应激障碍发生率较高,内向性格及存在偏瘫和吞咽功能障碍的患者更容易发生急性应激障碍。医护人员应及时为高危患者提供个体化治疗及预见性护理,防止脑卒中患者发生急性应激障碍。 相似文献
8.
9.