脑星形细胞瘤中TIP30、VEGF和CD34的表达及其相关性研究

Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.     

脑星形细胞瘤中TIP30、VEGF和CD34的表达及其相关性研究

Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.     

脑星形细胞瘤中TIP30、VEGF和CD34的表达及其相关性研究

Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.     

脑星形细胞瘤中TIP30、VEGF和CD34的表达及其相关性研究

Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.     

Artemin和基质金属蛋白酶2在食管鳞癌中的表达及其临床意义

目的 通过检测artemin和基质金属酶2(MMP2)在食管鳞癌中的表达水平,探讨其与食管癌的生长、浸润及转移之间的关系.方法 采用免疫组化的方法,对126例食管鳞癌患者手术切除的标本进行artemin和MMP2检测,并分析其临床病理意义.结果 Artemin与患者的TNM分期及淋巴结转移有密切的关系(P<0.05),与病变的分化程度、病变长度无明显的关系(P＞0.05).而且artemin与MMP2的表达呈正相关(r=0.551,P<0.01).结论 Artemin的表达与食管癌的病理分期、淋巴结转移和病变的分化程度有密切的关系,artemin与MMP2表达呈正相关,可以作为临床评估食管癌进展和预后的指标.     

Artemin在食管癌中表达作用的研究

目的:探讨Artemin(ATRN)在食管癌中的表达及作用.方法:采用免疫组化和real-time PCR方法检测ATRN在食管癌组织126例、癌旁组织126例和正常组织21例中的表达水平;应用siRNA技术在食管癌TE11细胞系中研究ATRN表达及对肿瘤细胞的凋亡影响.结果:126例食管癌中ATRN的阳性表达率为73.81%(93/126),癌旁组织的阳性表达率为44.44%(56/126);21例正常食管组织中的ATRN阳性表达率为14.29%(3/21),正常食管组织与食管癌组织及癌旁组织比较差异均有统计学意义(x2分别为32.89、23.15.均P＜0.01);TE11-siRNA细胞系ATRN的mRNA和蛋白表达水平均低于TE11-CK细胞系中相应的表达水平.siRNA抑制食管癌细胞中ATRN的表达后,促进了TE11细胞的凋亡.结论:ATRN与食管癌的生物学行为有关.     

小脑神经节细胞胶质瘤1例

脑神经节细胞胶质瘤(ganglioglioma)是发生于中枢神经系统中罕见的神经元和胶质细胞混合性肿瘤,其发生率占中枢神经系统肿瘤的0.4%,占脑肿瘤的1.3%.肿瘤好发于儿童和青年,60.0%在30岁以内.本文是对一患者小脑神经节细胞胶质瘤的诊断与治疗的观察.     

单发胃息肉样病变内镜特点和病理特征

目的：观察单发胃息肉样病变的内镜和病理形态学特征,探讨不同类型胃息肉的增殖和分化特征。方法分析661例单发性胃息肉样病变临床病理特点,免疫组织化学 EliVisionTMplus 两步法检测63例各类型胃息肉增殖细胞核抗原（Ki67）,细胞角蛋白7（CK7）,细胞角蛋白20（CK20）的表达。结果内镜诊断661例单发胃息肉样病变多为炎症,只有34．9％（231／661）诊断为胃息肉;单发性胃息肉样病变主要发生于胃窦;其中增生性息肉157例,在息肉中所占比例最高,多发生在胃窦部和贲门;胃腺瘤仅7例,主要发生于胃窦;除炎性纤维性息肉外,胃息肉的腺上皮均有不同程度 Ki-67表达,胃腺瘤的 Ki-67标记指数明显高于其他胃息肉（P ＜0．05）;胃息肉腺上皮免疫表型以CK7＋／CK20＋为主。结论胃息肉临床病理诊断符合率较低;不同类型胃息肉增殖指数不同,但免疫表型相似。     

Artemin在食管癌中表达作用的研究﹡

目的：探讨 Artemin（ATRN）在食管癌中的表达及作用。方法： 采用免疫组化和 real-time PCR 方法检测ATRN 在食管癌组织 126 例、癌旁组织 126 例和正常组织 21 例中的表达水平;应用 siRNA 技术在食管癌 TE11 细胞系中研究ATRN 表达及对肿瘤细胞的凋亡影响。结果： 126 例食管癌中ATRN 的阳性表达率为73.81% （93/126） ,癌旁组织的阳性表达率为44.44% （56/126） ; 21 例正常食管组织中的ATRN 阳性表达率为14.29% （3/21）,正常食管组织与食管癌组织及癌旁组织比较差异均有统计学意义 （字2分别为 32.89、 23.15, 均 P 约 0.01） ; TE11-siRNA 细胞系 ATRN 的mRNA 和蛋白表达水平均低于 TE11-CK细胞系中相应的表达水平。siRNA 抑制食管癌细胞中 ATRN 的表达后, 促进了 TE11 细胞的凋亡。结论： ATRN 与食管癌的生物学行为有关。