人原发胃癌、裸鼠人胃癌移植瘤及人胃癌细胞株中β-葡萄糖醛酸酶及神经氨酸苷酸活力比较

β-葡萄糖醛酸酶(β-G)主要存在于溶酶体,细胞癌变时溶酶体增生肥大,β-G合成增加,水解蛋白多糖破坏间质,促进癌细胞浸润生长和转移。神经氨酸苷酶(NANase)是糖蛋白、糖脂代谢的重要酶,细胞癌变时,细胞膜糖蛋白、糖脂所结合的寡糖链结构和成份发生改变,NANase活力亦相应发生变化。胃癌患者体液和红细胞膜上β-G及NANase活力变化情况已有报道结果表明上述酶活力与胃癌癌变关系密切,本文对人胃癌组织、人胃癌移植瘤及人胃癌细胞株中酶活力的变化进行探讨。     

ESTABLISHMENT AND BEHAVIOR OF A TRANSPLANTED HUMAN PRIMARY GASTRIC CANCER IN NUDE MICE

A human primary gastric cancer tissue (adenocarcinoma Ⅱ—Ⅲ) was transplanted into nude mice (SWISS/DF, nu/nu). It has been transfered for 8 generations at 56 sites in 28 nude mice with transplanting rate of 100%. The transplanted tumor is designated as transplanted human primary gastric cancer-1 in nude mice (THPGC-1). The growth rate of THPGC-1 is rather rapid and the size of transplanted tumor reaches 1cm~2 in 4—5 weeks after transfer. The morphology and histochemistry of the original tumor were retained well in the initial and serial transplanted tumors. THPGC-1 could secrete carcinoembryonic antigen (CEA). After intravenous or intraperitoneal injection of ~(131)Ⅰ-antiCEA monoclonal antibody into the THPGC-1 bearing nude mice, the radiolabeled antibody was concentrated and localized in the tumor as shown by γ-camera analysis. Similar pattern of lactate dehydrogenase isoenzyme was observed both in primary gastric cancer tissue and THPGC-1 tissue. Chromosomal examination revealed that THPGC-1 was human aneuploid ones. Southern blot analysis showed that the pattern of repetitive DNA bands and the structure of 28S rDNA, c-H-ras and c-myc genes in THPGC-1 were identical to the original primary gastric cancer DNA. The results suggest that THPGC-1 be a reliable model for the research of the molecular biology of cancer cells and experimantal gastric cancer diagnosis and treatment.     

维甲酸抑制人胃癌细胞株层粘素受体基因和原癌基因C—Ha—ras mRNA的表达

研究维甲酸（RA）对人胃癌细胞株（SGC7901）层粘素受体（LNR）基因和原癌基因C－Ha-ras表达的影响。方法：应用原位交方法标记LNR探讨和C－Ha-ras探针，对人胃癌细胞（分为经RA处理及未经RA处理两组）中LNR和C－Ha-ras基因的mRNA表达进行检测。结果：经RA处理的人胃癌细胞LNRmRNA表达较未经RA处理者显著降低（P＜0．01－0．001）；C－Ha-ras转录水平亦显著降低（P＜0．01－0．001）。结论：RA可显著抑制人胃癌细胞LNR和C－Ha-ras基因的表达。，     

我院人事制度改革的实践

近两年来，我基础医学院的劳动人事制度改革与我校进行的全面改革一样取得了一些成绩。为巩固成绩，以期求改革的深化和事业的继续发展，我们对前阶段改革的实践作了认真回顾和总结。一、改革实践的回顾1.确立改革目标。改革初始，多数人认为，当前人浮于事，极力主张“裁减冗员”，似乎这就是我们改革的目的与指导思想。而我们在调查中发现，有的教研室“人不多，事不少”，却存在着“有人无事做”；有的教研室“人很多，事很少”，也依然存在着”有事无人做”的现象。究其原因，关键在于缺少一种有效利用人力资源的机制。显然，如一味追求…     

激素调节大鼠间质细胞中11β—羟类固醇脱氢酶活性

糖皮质激素（大鼠体内是皮质酮）抑制睾丸间质细胞中睾酮合成。１１β－羟类固醇脱氢酶（１１β－ＨＳＤ）通过控制细胞内糖皮质激素浓度调节睾酮合成,并且其活性又能被大鼠内源性皮质酮诱导,由此保护了间质细胞中睾酮的生物合成,使之免于受到糖皮质激素的过度抑制。本研究旨在观察大鼠间质细胞中受１１β－ＨＳＤ调节的两种激素———皮质酮和睾酮是否对培养的大鼠间质细胞中１１β－ＨＳＤ活性及其ｍＲＮＡ表达具有调节作用。结果表明,皮质酮能增加切除肾上腺大鼠培养的间质细胞中１１β－ＨＳＤ氧化酶活性及其ｍＲＮＡ表达。睾酮则明显抑制培养的大鼠间质细胞中１１β－ＨＳＤ氧化酶活性及其ｍＲＮＡ表达。