支气管扩张症肺组织内降钙素表达的临床意义

目的　了解支气管扩张症肺组织降钙素 (CT)蛋白表达水平与其病变特点的关系。方法　应用免疫组化方法定量分析 4 5例支气管扩张症患者手术切除的肺组织 ,对其气道上皮内神经内分泌细胞 (NEC)计数 ,并与 19例无肺部疾患的尸检肺组织进行比较。结果　 2组均可见被染成棕红色的CT阳性细胞 ,它们分布于肺内各级支气管上皮细胞间 ,以支气管分叉处较多见。支气管扩张症组肺组织内5 0 0 0个上皮细胞中CT阳性细胞数为 (6 7.4 8± 11.0 3)个 ,非支气管扩张症组肺组织内CT阳性细胞数仅为 (5 .0 2± 1.0 0 )个 ,2组比较有非常显著性差异 (P <0 .0 0 1)。结论　支气管扩张症肺组织高水平CT表达可能与其慢性炎症改变有关 ,其局部CT表达增高可能系机体对支气管扩张病变的一种局部性代偿或调节机制。     

差示光谱法测定六味地黄丸中丹皮酚的含量

目的：用差示光谱法测定六味地黄丸中丹皮酚的含量。方法：采用乙醇浸出提取，缺丹皮酚样品为空白样品。以蒸馏水和０．０１ｍｏｌ·Ｌ－１氢氧化钠溶液分别定容，选择３５２ｎｍ为测定波长，３７０ｎｍ为参比波长。结果：丹皮酚浓度在６～１８μｇ·ｍｌ－１范围内ΔＡ与溶液浓度呈线性关系（ｒ＝０．９９９８），回收率为１０１．７％。结论：该法能有效地消除六味地黄丸中其它成分对丹皮酚含量测定的影响，而且简便、快速，结果满意     

Neuroprotective effects of Longxue Tongluo Capsule on ischemic stroke rats revealed by LC-MS/MS-based metabolomics approach

Objective: The present study aimed to evaluate the therapeutic effect and explore the underlying mechanisms of Longxue Tongluo Capsule (LTC) on ischemic stroke rats. Methods: Twenty-six rats were randomly divided into four groups, including sham group, sham + LTC group, MCAO group, and MCAO + LTC group. Ischemic stroke rats were simulated by middle cerebral artery occlusion (MCAO), and LTC treatment group were orally administrated with 300 mg/kg of LTC once daily for seven consecutive days. LTC therapy was validated in terms of neurobehavioral abnormality evaluation, cerebral infarct area, and histological assessments. The plasma metabolome comparisons amongst different groups were conducted by UHPLC-Q Exactive MS in combination with subsequent multivariate statistical analysis, aiming to finding the molecules in respond to the surgery or LTC treatment. Results: Intragastric administration of LTC significantly decreased not only the neurobehavioral abnormality scores but also the cerebral infarct area of MCAO rats. The interstitial edema, atrophy, and pyknosis of glial and neuronal cells occurred in the infarcted area, core area, and marginal area of cerebral cortex were improved after LTC treatment. A total of 13 potential biomarkers were observed, and Youden index of 11 biomarkers such as LysoPC, SM, and PE were more than 0.7, which were involved in neuroprotective process. The correlation and pathway analysis showed that LTC was beneficial to ischemic stroke rats via regulating glycerophospholipid and sphingolipid metabolism, together with nicotinate and nicotinamide metabolism. Heatmap and ternary analysis indicated the synergistic effect of carbohydrates and lipids may be induced by flavonoid intake from LTC. Conclusion: The present study could provide evidence that metabolomics, as systematic approach, revealed its capacity to evaluate the holistic efficacy of TCM, and investigate the molecular mechanism underlying the clinical treatment of LTC on ischemic stroke.     

基于点云数据的髋臼置钉通道全局优化

针对髋臼前后柱置钉通道多截面调控规划法的不足,该研究提出了基于大密度点云数据、相关理论、软件内置功能及自编程序的规划方法,实现了对置钉通道总长的全局优化,同时进行了基于7截面、11截面调控规划法以及点云数据的前柱最大内接圆柱体虚拟置钉实验,分析了实验结果及形成机理,验证了基于点云数据规划法的全局优化效果,但对多截面调控规划法的调控有效性表示还存有疑问,此外,还对基于点云数据的髋臼置钉通道规划方法应用的相关原理和操作技巧作了简略介绍。     

先秦社会时空方位观对中医理论的影响

先秦社会的五行阴阳以及四风系统的时空方位观对中医理论发展有着很大影响 ,说明中医理论是在接受先秦时期科学技术思想及社会观念发展起来的 ,有许多内容尚待探讨 ,如仅用秦汉后的观点去认识是会有偏误的。     

永存动脉干病理分型及临床诊断探讨

目的　通过临床和心血管造影检查资料分析探讨 39例永存动脉干 (TAC)的病理分型及临床诊断。方法　 39例TAC中 ,年龄 8个月至 2 5岁 ,平均 (7 9± 6 0 )岁。每例均经详问病史、常规体检、心电图、X线胸片和二维超声心动图检查及右心室和动脉干造影检查作出TAC的诊断。按Collett和Edwards分型方法分型 ,并结合VanPraagh分型进行讨论。 结果　按Collett和Edwards分型 ,Ⅰ型 4例 ,Ⅱ型 4例 ,Ⅲ型 3例 ,Ⅳ型 2 8例。结论　根据TAC定义 ,结合Tucuer的报道 ,Collett和Edwards分型的Ⅳ型可能为法洛四联症伴肺动脉闭锁 (假性动脉干 ) ,按VanPraagh分型更为合理。体循环侧支造影或电子束CT检查 ,在确定Collett和Edwards分型的Ⅳ型TAC是否为假性动脉干方面有重要价值。     

舒筋活络洗剂治疗骨折后关节挛缩的临床研究

为客观评价舒筋活络洗剂治疗骨折后关节挛缩的作用及不良反应.将100例骨折后关节挛缩的患者随机均分为治疗组和对照组,治疗组用舒筋活络洗剂,对照组有红花油,分别于用药后3、5、7天进行评分,并将评分结果统计分析.结果显示:①治疗组显愈率为88.00%,对照组显愈率为54.00%,两组比较(P《0.01),治疗组对于症候的改善作用优于对照组;②舒筋活络洗剂无毒副作用.表明舒筋活络洗剂是治疗骨折后关节挛缩有效而且安全的药物.     

光动力诱导前部缺血性视神经病变的血浆内皮素-1浓度变化的实验研究

目的观察通过光动力方法诱导鼠前部缺血性视神经病变模型(rAION)与正常鼠的血浆内皮素-1(ET-1)的浓度变化,探讨不同时间段血浆ET-1水平及与rAION水肿的关系,观察血浆ET-1的浓度变化与临床表现是否有同一性。方法依据光动力方法诱导rAION模型,应用SD雄性大鼠40只,随机分为2组:正常对照组20只和模型组20只,模型组再按照不同时间段分组各4只,造模后大鼠自由进食饮水,按第1天、第3天、第5天、第7天、第14天等时间段处死大鼠,心脏采血,离心后分离血浆,通过放射免疫法(RIA)测定血浆ET-1的浓度变化。结果对照组与模型组之间血浆ET-1值有统计学意义(P<0.05);模型组第1天、第3天、第5天、第7天、第14天时间段血浆ET-1值不同时间组之间差别有统计学意义(P<0.05)。结论 SD大鼠造模后血浆ET-l水平短期升高,第7天达到高峰,随后有所下降,但持续在一个高水平。血浆ET-1早期高表达可能是导致缺血性视神经病变视盘水肿增加的一个原因。血浆ET-1的浓度变化与临床表现具有同一性。     

他汀类药物对癌症患者预后的 Meta 分析(英文)

目的：系统评价癌症患者服用他汀类药物后对疾病预后的作用。方法：利用计算机检索Cochrane Library, PubMed和EMbase数据库,收集这些数据库中从建库至2015年3月公开发表的有关他汀类药物和癌症预后的文章,采 用Stata 12.0软件进行Meta分析。结果：共纳入25篇文献,523 193例患者。Meta分析结果显示：与未服用他汀类药物的 癌症患者相比,服用他汀类药物的癌症患者的总体死亡危险比(hazard ratio,HR)为0.82(95% CI：0.76~0.89)。亚组分析 结果显示前列腺癌症患者死亡的HR为0.66(95% CI：0.52~0.83)。敏感性分析显示在排除随访时间<5年的文献后,服用 他汀类药物的癌症患者的死亡HR为0.91(95% CI：0.75~1.10)。结论：他汀类药物能在一定程度上降低癌症患者的病死 率,但其远期作用还需要进一步研究确认。     

Increased expression of HMGB3: a novel independent prognostic marker of worse outcome in patients with esophageal squamous cell carcinoma

HMGB3, an X-linked member of the high-mobility group (HMG) superfamily of HMG proteins, has been shown to affect numerous tumorigenic progression. However, the expression and the prognostic role of HMGB3 in esophageal squamous cell carcinoma (ESCC) remained unknown. In this study, we examined the HMGB3 expression in ESCC tissues and adjacent nontumorous tissues by qRT-PCR and immuohistochemistry. Statistical analyses were applied to test for prognostic and diagnostic associations. The mRNA levels of HMGB3 were found to be significantly higher in tumorous tissues than in the adjacent normal tissues. We found that the HMGB3 expression was higher in tumorous tissues than in the adjacent non-tumorous tissues by immunohistochemical analysis of paired tissue specimens (P < 0.001). Moreover, there was a significant correlation between HMGB3 expression and gender (P = 0.037), clinical stage (P = 0.038), T classification (P = 0.013) and N classification (P = 0.017). Patients with higher HMGB3 expression had shorter overall survival than those with lower HMGB3 expression. Multivariate Cox analysis indicated that HMGB3 expression is an independent prognostic factor for overall survival (HR = 0.591, 95% CI = 0.379-0.793, P = 0.039). In summary, these findings demonstrate that HMBG3 may be a potential molecular marker for predicting the prognosis of ESCC patients.