足三里穴治疗胸痹的辨证分型及分析

目的:采用文献计量学对近20年来针灸足三里穴治疗胸痹的辨证分型进行分析,探讨分析足三里穴在胸痹中的治疗作用,为临床优化腧穴选穴提供一定的参考.方法:通过回顾1999年1月至2019年1月针灸足三里治疗心绞痛的临床文献,对足三里穴在治疗胸痹中的辨证分型进行定量分析,研究各证型出现的频率、比例及统计学差异.结果:共纳入43...     

带脉探析

带脉起于季胁,回身一周,有约束纵行诸经的功能.带脉与督脉、任脉、冲脉、足厥阴肝经、足少阳胆经、足太阴脾经、足阳明胃经、足少阴肾经直接或间接的产生联系;与之相关的腧穴分别为章门、带脉、五枢、维道、肾俞、足临泣.从带脉论治腰部疾病、肥胖、消渴和痿证,能够取得良好的效果.     

电针预处理对心肌缺血再灌注大鼠线粒体能量代谢的影响

ObjectiveTo explore the effects of electroacupuncture pretreatment on mitochondrial energy metabolism in the rats with myocardial ischemia reperfusion injury (MIRI).MethodsA total of 60 SPF Wistar rats were randomly divided into a sham-operation group (sham group), a myocardial ischemia reperfusion injury group (MIRI group) and an electroacupuncture pretreatment group (EA group), 20 rats in each one. The rats in the sham group and the MIRI group were binded for 7 days, once a day, 20 min each time. On the 8^th day, the sample was collected after the heart exposed for 50 min in thoractomy in the sham group and the sample was collected after ischemia for 20 min and reperfusion for 30 min in thoractomy in the MIRI group. In the EA group, the pretreatment intervention with electroacupuncture was applied at "Nèiguān (内关PC6)", "Guānyuán (关元CV4)" and "Zúsānlĭ (足三里ST36)" in the rats for 7 days, once a day, 20 min each time. On the 8^th day, after ischemia for 20 min and reperfusion for 30 min in thoractomy, the sample was collected in the EA group. The changes in ST_Ⅱ segment of electroacardiogram (ECG) were observed and measured. Using enzyme-linked immunosorbent assay (ELISA), the concentrations of cardiac troponin T (cTnT) and cardiac troponin I (cTnI)were detected. Using nitro blue tetrazolium chloride monohydrate (NBT) staining, the myocardial infarction weight percentage was measured. Using ELISA, the concentrations of mitochondrial adenosine monophosphate (AMP), adenosine diphosphate (ADP)and adenosine triphosphate (ATP)were detected.Results(1) ST_Ⅱ changes: in 20 min of ligation, compared with the sham group, the ST_Ⅱ segment of electrocardiograph (ECG) was elevated significantly in the MIRI group and EA group (both P < 0.01), but the elevation range in the EA group was lower than that of the MIRI group (P < 0.01). After reperfusion for 30 min, the ST_Ⅱ segment was fallen by over 50% in the MIRI group and the EA group. Simultaneously, the ST_Ⅱ segment in the EA group was lower than that of the MIRI group (P < 0.01). (2) Regarding myocardial infarction weight percentage, compared with the sham group, the infarction weight was larger in the MIRI group and the EA group (both P < 0.05) and the infarction weight in the EA group was lower than that of the MIRI group (P < 0.05). (3) Regarding the levels of serum cTnt and cTnI, compared with the sham group, the levels of serum cTnT and cTnI were higher in the MIRI group and the EA group (all P < 0.01) and the levels of cTnT and cTnI in the EA group were lower than that of the MIRI group (both P < 0.01). (4) Regarding the concentrations of AMP, ADP and ATP, compared with the sham group, ATP concentration was lower in the MIRI group and the EA group (both P < 0.01) and the concentrations of AMP and ADP were higher (P < 0.05, P < 0.01). Compared with the MIRI group, ATP concentration was higher in the EA group (P < 0.05) and the concentrations of AMP and ADP were lower (both P < 0.01).ConclusionsElectroacupuncture pretreatment reduces the elevation of ECG ST_Ⅱ segment, decreases the concentrations of myocardial injury markers, cTnT and cTnI and regulates the transfer among AMP, ATP and ADP. The protective effect of electroacupuncture pretreatment may result from the regulation of mitochondrial energy metabolism.     

电针预处理对心肌缺血再灌注损伤大鼠线粒体介导的细胞凋亡的影响

目的:探讨电针预处理对心肌缺血再灌注损伤大鼠的线粒体膜电位(△Ψm)及其介导的细胞凋亡的影响。方法:将60只SPF级雄性Wistar大鼠随机分为假手术组(Sham)、缺血再灌注组(MIRI)和电针预处理组(EA),每组20只。Sham组、MIRI组均采用自制鼠衣捆绑,固定于自制鼠台7天,1次/天,20min/次,第8天,Sham组开胸暴露心脏50min后、MIRI组开胸缺血20min再灌注30min后取材。EA组,电针预处理"内关"(双侧)、"足三里"(双侧)、"关元"7天,1次/天,20min/次,第8天开胸缺血20min,再灌注30min后取材。采用TTC染色法测定缺血再灌注损伤后心肌梗死的面积和重量,采用JC-1染色法检测△Ψm的水平,采用实时荧光定量PCR法检测第二个线粒体衍生的半胱氨酸蛋白酶激活剂(Smac/Diablo)、天冬氨酸特异性半胱氨酸蛋白酶-7(Caspase-7)、天冬氨酸特异性半胱氨酸蛋白酶-9(Caspase-9)基因表达水平。结果:(1)心肌梗死面积和重量:与Sham组相比,MIRI组和EA组的梗死面积和重量均增加(均P0.05),且EA组的梗死面积和重量低于MIRI组(均P0.01)。(2)线粒体膜电位:与Sham组相比,MIRI组和EA组的红/绿荧光比值均明显下降(均P0.01);与MIRI组比较,EA组红/绿荧光比值明显升高(P0.01)。(3)Smac/Diablo基因表达水平:与Sham组相比,MIRI组和EA组的Smac/Diablo、Caspase-7、Caspase-9的基因表达水平显著升高(均P0.01);与MIRI组相比,EA组的Smac/Diablo、Caspase-7、Caspase-9基因表达明显下降(均P0.01)。结论:电针预处理可有效缩小心肌梗死范围,提高线粒体膜电位水平,下调促凋亡基因Smac/Diablo、Caspase-7、Caspase-9的表达,电针预处理的保护作用可能是基于改善线粒体膜电位水平、抑制Smac/Diablo介导的线粒体Caspase凋亡通路产生的。     

基于FXR/SHP通路探讨电针预处理对心肌缺血再灌注损伤大鼠心肌组织相关凋亡基因的影响

目的:观察电针预处理对心肌缺血再灌注损伤(MIRI)大鼠法尼酯衍生物X受体(FXR)/小异二聚体配体(SHP)凋亡通路及凋亡诱导因子(AIF)和热休克蛋白70(HSP70)的影响,探讨电针预处理改善M IRI的作用机制.方法:Wistar大鼠随机分为正常对照组、假手术组、缺血再灌注组、电针预处理组,每组10只.采用结扎...