中医辨证分期治疗视网膜静脉阻塞86例

目的:观察应用中医辨证分期治疗视网膜静脉阻塞的临床疗效.方法:对86例视网膜静脉阻塞患者初期应用生蒲黄汤加减、中期应用通窍活血汤加减、后期应用驻景丸加减治疗.结果:显效32例,有效40例,无效14例.结论:中医辨证分期治疗可以有效的治疗视网膜静脉阻塞.     

Damaging effects of polychlorinated biphenyls on chicken primordial germ cells

This work describes the effects of a commercial polychlorinated biphenyl (PCB) mixture, Aroclor 1254, as well as 17beta-oestradiol (E2) and testosterone on numbers and histomorphological changes of primordial germ cells (PGCs) in gonadal regions of Day 5 Hyline chicken embryos. The oestrogen receptor antagonist, clomiphen, alone or with PCBs was used in an attempt to protect the developing gonad from oestrogen-like effects of chemical PCBs. The results were as follows: (i) PCBs delayed embryonic development independently of dose (1 microg/egg, P<0.05; 10 microg/egg, P<0.01; 100 microg/egg, P<0.001 v. the control) and caused a dose-independent increase in mortality compared with the control group (10 microg/egg, P<0.01; 100 microg/egg, P<0.05); maximal mortality was observed in the 1 microg/egg group (P<0.001); (ii) PCBs decreased PGC numbers dose dependently (P<0.001) and caused a swollen nucleus with hyperchromatism (pyknosis) or cytoplasm vacuolation as signs of gonadal PGC degeneration in all PCB groups, or even complete disappearance in the 100 microg/egg group; (iii) after PCB treatment, the index of gonadal lesion increased significantly with the decrease of gonadal PGC number (1, 10 and 100 microg/egg, P<0.001); (iv) there were no observed effects of E2, testosterone and clomiphen on PGCs in the experiments; and (v) clomiphen failed to block the damaging effects of PCBs. These results suggest that the adverse effects of PCBs on chicken gonadal and germ cell development were initiated during the early stages of incubation through direct toxic effects, rather than through oestrogen-mimicking actions. As PGC numbers in the gonads decrease and the index of gonadal lesion increases, one may expect reproductive function to be compromised.     

抗抑郁治疗对功能性消化不良及水负荷的影响

目的 :探讨心理因素和水负荷实验与功能性消化不良的关系。方法 :对 5 3例FD组行氟西汀治疗 6周。使用HAMD评分和水负荷实验对治疗前后 5 3例FD实验组以及 2 3例对照组的精神状态和胃功能进行评估。结果 :FD组治疗前后进行HAMD(2 4项 )评分 ,治疗有效率为 84 .91% (45 /5 3)。治疗前FD组的HAMD评分 2 9.6 8± 9.2 7显著高于对照组 4 .6 8± 2 .73,P <0 .0 1;FD组的水负荷量 10 35± 35 3ml低于对照组 2 330± 135ml,两者差异显著 ,P <0 .0 1。治疗前HAMD评分 2 9.6 8± 9.2 7分明显高于治疗后 12 .4 3± 8.4 3分 ,P <0 .0 5 ;治疗前的水负荷量 10 35± 35 3ml明显低于治疗后的 2 0 10± 330ml,P <0 .0 5。结论 :对于FD患者 ,HAMD积分和水负荷实验是安全、简单、可靠的方法 ,既可反映心理因素在FD中的作用 ,又能评价当时FD患者的近端胃功能 ,为FD的抗抑郁疗法提供了重要理论基础。     

Lactate dehydrogenase A negatively regulated by miRNAs promotes aerobic glycolysis and is increased in colorectal cancer

Reprogramming metabolism of tumor cells is a hallmark of cancer. Lactate dehydrogenase A (LDHA) is frequently overexpressed in tumor cells. Previous studies has shown higher levels of LDHA is related with colorectal cancer (CRC), but its role in tumor maintenance and underlying molecular mechanisms has not been established. Here, we investigated miRNAs-induced changes in LDHA expression. We reported that colorectal cancer express higher levels of LDHA compared with adjacent normal tissue. Knockdown of LDHA resulted in decreased lactate and ATP production, and glucose uptake. Colorectal cancer cells with knockdown of LDHA had much slower growth rate than control cells. Furthermore, we found that miR-34a, miR-34c, miR-369-3p, miR-374a, and miR-4524a/b target LDHA and regulate glycolysis in cancer cells. There is a negative correlation between these miRNAs and LDHA expression in colorectal cancer tissues. More importantly, we identified a genetic loci newly associated with increased colorectal cancer progression, rs18407893 at 11p15.4 (in 3′-UTR of LDHA), which maps to the seed sequence recognized by miR-374a. Cancer cells overexpressed miR-374a has decreased levels of LDHA compared with miR-374a-MUT (rs18407893 at 11p15.4). Taken together, these novel findings provide more therapeutic approaches to the Warburg effect and therapeutic targets of cancer energy metabolism.     

苓桂术甘汤加味治疗百日咳重症痉咳156例

笔者对156例百日咳重症痉咳患儿,采用脾肺同治法,以张仲景苓桂术甘汤加浙贝母、百部、旋复花、枳壳、桃仁、地龙等为基础方剂辨证治疗。治疗结果:1周内咳嗽及随症消失者148例(95％):5天内咳减,10天内咳嗽基本消失者6例(3.8％);无效者2例(1.2％),总有效率98.8％。     

灯盏细辛注射液中总咖啡酸酯在大鼠体内的药代动力学研究

目的:研究灯盏细辛注射液中总咖啡酸酯的大鼠体内过程。方法:采用甲醇沉淀法制备血液样品,并用UV法测定。结果:浓度在20~2μg/ml范围内线性关系良好,相关系数r=0.995;样品的回收率在94%~100.8%,日内RSD和日间RSD均小于5%。总咖啡酸酯在大鼠体内的过程符合二室模型(weight=1/c/c),主要药动学参数为:α=0.1595±0.0658min-1,β=0.0037±0.0014min-1,V(c)=18.0198±2.0215(mg/kg)/(mg/ml),T1/2α=4.3456±1.2854min,T1/2β=188.6554±10.2556min,K21=0.0212±0.0046min-1,K10=0.0275±0.0089min-1,K12=0.1143±0.0594min-1,AUC=59.8192±4.1564(mg/ml).min。结论:灯盏细辛注射液中的总咖啡酯类成分在大鼠体内过程符合静脉注射二室模型。     

公立医院行政管理人员职业发展规划管理研究

目的通过对行政管理人员职业生涯规划管理现状的调查,探讨影响职业生涯规划管理的因素,针对行政管理人员的工作特点和医院工作实际,提出适合医院及行政管理人员特征的公立医院行政管理人员职业生涯规划管理的意见和建议。方法选取广州、北京、上海、江苏等经济发达地区的12家三级公立医院行政管理人员进行现场多级分层随机抽样,通过采用自行编制的调查表对公立医院行政管理人员的职业生涯规划情况进行调查。对调查表采用Visual Foxpro建立数据库并输入资料,利用SPSS 11.3进行数据录入,统计分析。结果医院对行政管理人员的职业生涯规划指导作用不够,职业生涯规划意识淡薄,缺少管理系列的职称晋升体系,不同学历、职称、职务的行政管理人员的工作岗位感知得分和职业生涯规划管理得分有差异等。结论医院行政管理人员的职业生涯规划管理处于发展阶段,医院应该建立起完善的行政管理人员职业生涯管理体系,针对不同人群采取差异性职业生涯规划管理措施。     

清热固肠止泻汤治疗放射性直肠炎临床观察

目的 :观察清热固肠止泻汤治疗放射性直肠炎疗效。方法 :将 33例放射性直肠炎患者随机分为两组 ,治疗组 2 1例 ,采用清热固肠止泻汤治疗。对照组采用甲硝唑、庆大霉素 6 5 4 - 2、利多卡因灌肠治疗 ;两组 1周为 1疗程 ,3个疗程后统计疗效。结果 :治疗组总有效率 85 .7% ,对照组总有效率 6 6 .7% ,两组对比有显著差异 (P <0 .0 1)。结论 :清热固肠止泻汤治疗放射性直肠炎疗效确切。     

Developing an effective polarizable bond method for small molecules with application to optimized molecular docking

Electrostatic interaction plays an essential role in protein–ligand binding. Due to the polarization effect, electrostatic interactions are largely impacted by their local environments. However, traditional force fields use fixed point charge–charge interactions to describe electrostatic interactions but is unable to include the polarization effect. The lack of the polarization effect in the force field representation can result in substantial error in biomolecular studies, such as molecular dynamics and molecular docking. Docking programs usually employ traditional force fields to estimate the binding energy between a ligand and a protein for pose selection or scoring. The intermolecular interaction energy mainly consists of van der Waals and electrostatic interaction in the force field representation. In the current study, we developed an Effective Polarizable Bond (EPB) method for small organic molecules and applied this EPB method to optimize protein–ligand docking in computational tests for a variety of protein–ligand systems. We tested the method on a set of 38 cocrystallized structures taken from the Protein Data Bank (PDB) and found that the maximum error was reduced from 7.98 Å to 2.03 Å when using EPB Dock, providing strong evidence that the use of EPB charges is important. We found that our optimized docking approach with EPB charges could improve the docking performance, sometimes dramatically, and the maximum error was reduced from 12.88 Å to 1.57 Å in Optimized Docking (in the case of 1fqx). The average RMSD decreased from 2.83 Å to 1.85 Å. Further investigations showed that the use of the EBP method could enhance intermolecular hydrogen bonding, which is a major contributing factor to improved docking performance. Developed tools for the calculation of the polarized ligand charge from a protein–ligand complex structure with the EPB method are freely available on GitHub (https://github.com/Xundrug/EPB).

Electrostatic interaction plays an essential role in protein–ligand binding.     

VEGF-B inhibits apoptosis via VEGFR-1-mediated suppression of the expression of BH3-only protein genes in mice and rats

Despite its early discovery and high sequence homology to the other VEGF family members, the biological functions of VEGF-B remain poorly understood. We revealed here a novel function for VEGF-B as a potent inhibitor of apoptosis. Using gene expression profiling of mouse primary aortic smooth muscle cells, and confirming the results by real-time PCR using mouse and rat cell lines, we showed that VEGF-B inhibited the expression of genes encoding the proapoptotic BH3-only proteins and other apoptosis- and cell death-related proteins, including p53 and members of the caspase family, via activation of VEGFR-1. Consistent with this, VEGF-B treatment rescued neurons from apoptosis in the retina and brain in mouse models of ocular neurodegenerative disorders and stroke, respectively. Interestingly, VEGF-B treatment at the dose effective for neuronal survival did not cause retinal neovascularization, suggesting that VEGF-B is the first member of the VEGF family that has a potent antiapoptotic effect while lacking a general angiogenic activity. These findings indicate that VEGF-B may potentially offer a new therapeutic option for the treatment of neurodegenerative diseases.