急性脑梗死血糖与临床症状的观察分析

目的:对急性脑梗死患者血糖增高与临床症状的观察。方法:急性脑梗死患者于入院时检测血糖并分组,入院时及入院6 d时进行神经功能缺损评分比较,并且观察14 d内死亡率及消化道出血率。结果:血糖升高组患者6 d时神经功能恢复情况差于血糖正常组(P <0 .0 5 ) ,14 d内死亡率及消化道出血率高于血糖正常组(P <0 .0 5 )。结论:急性脑梗死患者血糖升高对早期临床症状改善不利,明显增加并发症及死亡率。     

低分子肝素在静脉溶栓治疗中的临床研究

目的:评价低分子肝素在脑梗死急性治疗中的作用。方法:将符合标准的80例患者随机分为治疗组(40例),对照组(40例),治疗组溶栓后再给以低分子肝素抗凝治疗,对照组给予常规溶栓治疗,于治疗前、治疗第3天、第7天、第15天时神经功能缺损评分比较,在第15天时两组疗效比较。结果:两组比较差异无统计学意义。结论:静脉溶栓后使用低分子肝素与对照组在痊愈率、显效率、有效率、病死率上无显著差异,在急性脑梗死的溶栓治疗中,低分子肝素的应用价值有待进一步研究。     

尿激酶治疗短时间反复发作TIA临床分析

目的：探讨短时间频繁发作的TIA患者治疗方式。方法：选取我科2005/2007年TIA患者各20例,治疗组在治疗基础上加尿激酶治疗与对照组比较。结果：两组疗效比较差异有显著性（P〈0.01）。结论：常规治疗加一定量尿激酶能有效控制TIA的频繁发作,对防止脑梗死的发生有良好疗效。     

蒙特利尔认知量表和MMSE量表在阿尔茨海默病筛查中的比较与应用

阿尔茨海默病(Alzheimer’s disease,AD)又称老年性痴呆,是一种神经系统退行性疾病[1],尚无特异的早期诊断标志。神经心理学量表主要应用在老年痴呆筛查工作中,是高级神经功能受损诊断、评估和康复的重要依据。蒙特利尔认     

血浆同型半胱氨酸水平与脑梗死并发2型糖尿病的相关研究

目的：探讨血浆同型半胱氨酸（Hcy）水平与动脉粥样硬化性血栓性脑梗塞合并2型糖尿病的关系。方法：选择动脉粥样硬化性血栓性脑梗塞合并2型糖尿病患者60例（A组）为患者组,动脉粥样硬化性血栓性脑梗塞无2型糖尿病病史患者60例（B组）,2型糖尿病无动脉粥样硬化性血栓性脑梗塞60例（C组）,健康人组60例（D组）B组、C组、D组均为对照组,用酶联免疫吸附法（ELISA）测定血浆同型半胱氨酸（Hcy）水平,用全自动生化分析仪检测总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDLC）、甘油三酯（TG）,并进行统计分析。结果：A组Hcy高于B组、C组、D组,有显著性差异（P〈0.05）,B组Hcy值高于C组、D组,有显著性差异（P〈0.05）,C组高于D组（P〈0.05）,有显著性差异。结论：Hcy在动脉粥样硬化性血栓性脑梗塞合并2型糖尿病过程中起着协同作用,是动脉粥样硬化性血栓性脑梗塞合并2型糖尿病的重要危险因素。血浆Hcy的升高可能与动脉粥样硬化性血栓性脑梗塞合并2型糖尿病有相关性。     

NGF对神经干细胞内Mash1表达的影响

目的:以Mash1为切入点,深入研究NGF反应性NSCs向胆碱能神经元分化过程中的机制。方法:取体外培养传至第三代的神经干细胞,在含不同浓度NGF的神经干细胞分化培养基中进行分化诱导,采用RT-PCR法测定诱导后Mash1的mRNA相对表达水平。结果:各实验组均有Mash1的表达,其中空白对照组表达最少,以NGF100mg/L组表达量最高。结论:NGF能够促进NSCs内Mash1的表达。     

比较四种消化方法对神经干细胞传代增殖及活性的影响

目的：观察4种消化方法对体外培养的神经干细胞（N SC s）增殖及活性的影响。方法：原代培养新生大鼠海马N SC s,采用机械消化、0.25%单纯胰酶、0.25%胰酶＋0.02%EDTA和0.02%EDTA 4种不同消化方法对N SC s球进行传代培养,经四唑盐比色法（M TT法）法检测不同方法对N SC s活性及增殖能力的变化。结果：与机械消化相比,0.25%单纯胰酶组,0.25%胰酶＋EDTA组消化后的大鼠海马N SC s球长时间不聚集,细胞增殖停顿,而单纯EDTA组则明显提高了N SC s的增殖及活性,其余各组差异无统计学意义。结论：随培养时间延长,单纯0.02%EDTA组能够显著提高体外培养的大鼠海马N SC s的增殖水平及活力,且对细胞的后续损伤相对较小。     

基底动脉尖综合征5例临床分析

基底动脉尖综合征(Topofthe Basilar Syndrome,TOBS),是由Caplan于1980年首先提出,临床较少见,主要是以神经障碍、视觉障碍和意识行为异常为特征的一组征侯群。现将2000-2004年我科收治的TOBS患者5例报道如下。     

肾虚及正常大鼠的正畸致牙根吸收易感性比较及组织内白细胞介素17的表达差异

BACKGROUND: The mechanism underlying orthodontic-induced external root resorption is not yet clear, and it differs individually. Kidney deficiency has been proved to be related to bone diseases which mediated by different cytokines. Interleukin-17 is an important cytokine involved in external root resorption. So figuring out whether kidney deficiency and interleukin-17 are related to root resorption will be helpful for etiological research. OBJECTIVE:To explore the relationship between kidney deficiency physique, interleukin-17 and root resorption during orthodontic treatment in rats. METHODS: Thirty-six Wistar rats were selected and equivalently randomized into two groups, followed by modeled into kidney deficiency (kidney deficiency group) or injected with normal saline (control group), respectively. Afterwards, the right maxillary of each rat served as an orthodontic force model, and the left maxillary as a non-orthodontic force model. All rats were respectively sacrificed under general anesthesia at the 3, 7 and 14 days after given orthodontic force. Then, the mesial surface of the root of maxillary first molars and the expression level of interleukin-17 were observed through hematoxylin-eosin staining and immunohistochemical method. RESULTS AND CONCLUSION: Histological observation showed that significantly increasing root resorption in a time-dependent manner could be observed, and there were various absorbed lacunae of osteoclasts on the enamel in the kidney deficiency orthodontic force group. The alveolar bone resorption and widened periodontal membrane appeared in the control orthodontic force group. While no remarkable root and alveolar bone resorptions were found in the other two non-orthodontic force groups. The expression level of interleukin-17 in the kidney deficiency orthodontic force group was higher than that in the control orthodontic force group; the expression level of interleukin-17 in the kidney deficiency non-orthodontic force group was higher than that in the control non-orthodontic force group. In conclusion, kidney deficiency patients are easy to develop root resorption, the mechanism of which is maybe relevant to the upregulation of interleukin-17. 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程