脂质体介导的单纯疱疹胸苷激酶基因在人前列腺癌细胞中的表达

目的：提取并鉴定已构建的EB病毒表达载体pDR2-TK，利用脂质体介导的基因转染技术将pDR2-TK转染人前列腺癌细胞并对单纯疱疹胸苷激酶（HSV－TK）表达状况进行检测。方法：采用DNA大量制备及纯化系统提取pDR2-TK，酶切和DNA测序进行鉴定，采用阳离子脂质体法将pDR2-TK导入激素非依赖性人前列腺癌细胞系PC－3m，逆转录PCR（RT－PCR）法和SABC免疫组化法检测TK mRNA和蛋白的表达。结果：扩增提取的质粒经PstⅠ和EcoR Ⅴ酶切后各获得4个及2个片段，与原基因酶切图谱一致；所提取质粒PCR产物经DNA测序，与NCBI公布的HSV－TK基因序列对照，证实所提取质粒含目的基因序,旨质体法转染PC－3m细胞后，mRNA和蛋白均有HSV－TK的表达，其蛋白表达率约为22％。结论：pDR2-TK质粒含有目的的基因HSV－TK，阳离子脂质体法可将pDR2-TK导入人前列腺癌细胞并获得较高效率的表达。     

过氧化脲治疗四环素牙的远期疗效

目的评价10％过氧化睬凝胶漂白剂家庭漂白（In-home bleaching）治疗四环素牙的远期临床效果。方法用含10％过氧化脲的凝胶漂白剂。通过可由患者自行取戴的漂白托盘治疗四环素牙，观察其脱色效果及牙色复原等变化情况。结果306例四环素牙患者漂白总满意率为86．6％；随访6个月的201例中，有14．9％于6个月后出现不同程度的颜色复原。1～2年后，仍有较高的满意率。其中30例。每隔2年做1次巩固治疗。随访11年临床效果良好。结论10％过氧化脲凝胶漂白剂治疗四环素牙是有效的方法，着色度越轻治疗效果越好，着色度越重治疗效果越差。同时，漂白治疗具有一定的复原性，坚持巩固治疗。远期效果令人满意。     

横向电场作用下外周神经兴奋特性的实验研究

传统的外周神经电缆方程只能描述纵向电场刺激下外周神经兴奋,实验发现在脉冲磁场诱导的横向电场作用下也可使神经兴奋,从而揭示出需要进一步对感应电场兴奋外周神经的机理进行研究。本文研究了横向电场作用下外周神经的兴奋特性,以在体的人体正中神经为例研究了横向电场作用下外周神经兴奋点的位置、刺激阈值、及刺激阈值与纤维半径的关系,以离体的蟾蜍坐骨神经为例研究了刺激阈值与作用时间的关系。实验结果验证了改进的电缆方程的有效性。实验研究成果有助于磁刺激技术的进一步发展与应用。     

淫羊藿、人参耐缺氧、耐疲劳及抗氧化作用的实验研究

目的 探讨淫羊藿、人参耐缺氧、耐疲劳及抗氧化作用。方法 采用单独和联合给药的方法对老龄小鼠进行抗缺氧实验,耐疲劳实验,按硫代巴比妥法测定血浆和组织MDA,按EistnerFF法测定红细胞SOD的活性。结果与结论 人参能提高小鼠耐缺氧的能力,淫羊藿可加强其作用;两者都能提高小鼠耐疲劳的能力。淫羊藿和人参均有抗氧化作用,可使血浆和组织中的MDA降低,红细胞的SOD活性增加。两药配伍使用有协同作用。     

Primary hepatic non-Hodgkin's lymphoma: a case report and review of the literature

~ hepatic lympholna (PHL) is very uncommon, with which most clinicians are not fallliar. We report a case Of p~ hepatic non-Hodgkin's lymphomawhoSe clinical ~stalons were Presented edng withP~ssiVe liVer failure, and the comet diagnosis wascoallrmed by liver biopsy. A review of the lite~re anddiscussion of PHL ~ also Presented.~ ~ ~sPatient A 32-year-old man was abbotted to ourdepallment on APril l, 1999 because of fatigUe, anowhaand loss Of weight for 5 months, feVer for 4 d. The onse…     

Comparison of survival among patients with breast cancer treated at First Teaching Hospital,Changchun, China,and Saint-Sacrement hospital,Quebec, Canada

The aim of this study was to compare the survival of 116 patients with breast cancer initially treated at the First Teaching Hospital (FTH) of Norman Bethune University of Medical Sciences located in Changchun, China, from 1986 to 1991 with the survival of 886 patients seen in the “Hipital du Saint-Sacrement” (HSS) located in Quebec City, Canada, from 1987 to 1992. The clinical data were collected from the hospital records at FTH. The vital status for Chinese patients was obtained from letters of follow-up or the records of local police offices. The list of patients treated at HSS and the data for each woman were extracted from computerized data banks. The major variables studied included age at diagnosis, tumor size at pathology (cm), number of lymph nodes involved, breast surgery and adjuvant treatments of breast cancer (chemotherapy, radiotherapy, immuno-therapy). Age at diagnosis was substantially lower among patients with breast cancer seen at FTH compared to those treated at HSS (x) ₁ ² =60.95,P<0.0001). The average age at diagnosis for Chinese women was about 10 years less than that for Canadian women. Patients in the two hospitals differed with respect to tumor size at pathology (x ₂ ² =6.67,P=0.036). The proportion of patients with tumor size larger than 2.0 cm was larger at FTH (48.3%) than at HSS (41.1%). The mean tumor size at pathology was 3.0 cm (standard deviation =2.1 cm) for patients treated at FTH, but 2.6 cm (standard deviation=1.8 cm) for women treated at HSS (P=0.07). The proportion of women with lymph node involvement was greater at FTH (61.1 % than that at HSS (37.3%) (x ₁ ² =16.51,P<0.0001). Surgical treatment of breast cancer varied considerably. In Changchun, radical mastectomy was frequent for any stage of breast cancer patients, but partial mastectomy was never performed. The situation was reversed in Quebec. The five year observed survival was 74.2% (standard error, 0.05) among breast cancer patients seen at FTH compared to 76.0% (standard error, 0.02) among women treated at HSS. After adjustments of confounding factors, there were no significant difference in five year observed survival between the patients treated at the two hospitals (P=0.42).     

复合法建立大鼠溃疡性结肠炎模型

为建立一种类似人类溃疡性结肠炎（UC）特点的动物模型,将60只封闭群健康成年Wistar大鼠随机分为3组造模,每组20只,A组应用2,4－二硝基氯苯（DNCB）和乙酸复合法,B组单纯应用DNCB,C组单纯应用乙酸,对3组进行对照研究。结果：病程：A组大于16周,B组2周,C组8周;免疫学反应：造模前及造模完成后1周CD4＋CD29＋细胞A组为5．01±2．01％,11．17±2．18％,P＜0．01;B组为4．96±1．87％,10．98±2．87％,P＜0．01;C组为4．93±1.96％,5．06±2．03％,P＞0．05;病理学改变：3种方法均可产生粘膜溃疡、炎性细胞浸润、隐窝脓肿等UC组织学特征;症状：3种方法均可造成大鼠腹泻、粘法血便等UC症状。结论：复合法大鼠UC模型既克服了DNCB病程短、自愈性强的缺点,也克服了乙酸法不具有免疫反应模型特征的缺点,又同时保留了二者症状、组织学改变与人类UC相似的优点,是一种较理想的新的UC模型。     

高效崩解剂对提高盐酸甲福明片剂质量的研究

 目的：为提高盐酸甲福明(metformin hydrochloride，MFH)片的质量，研究应用高效崩解剂微晶纤维素等考查其作用并进行评价。方法：应用微晶纤维素，L-HPC和交联PVP，交联CMC-Na，CMS-Na为崩解剂设计和制备了6种处方的MFH片剂。测定和对照片芯的硬度，崩解时限，溶出速度和颗粒性质。结果：各处方的片剂硬度均达到8kg以上。含有交联PVP和交联CMC-Na的片剂崩解时限在3min以内。应用高效崩解剂交联PVP和交联CMC-Na的片剂5min内全部溶出。采用L-HPC和微晶纤维素为崩解剂10min内药物溶出可达95%以上。结论：在处方中改用4%的交联PVP，交联CMC-Na，L-HPC和CMC-Na作崩解剂能得到优质的MFH片剂。     

基于XML三层C／S模型在HIS系统建设中的应用

本文说明了目前比较流行的三层架构的网络体系、XML(Extensible Markup Language，可扩展标志语言)语言的优势和医院信息系统(Hospital Inforination Systein，简称HIS系统)的特点，指出了XML语言和C／S(Client／Server,客户／服务器)模型在HIS系统中应用能够使HIS系统的功能得到很大的提高。     

深圳市艾滋病分子流行病学和HIV膜蛋白基因变异与感染时间相关性分析

目的:对深圳市HIV感染流行趋势分析,为深圳市艾滋病防治策略提供依据.方法:对1992～2000年深圳市HIV血清学监测质料及应用套式PCR(nested-PCR)对深圳市55例HIV感染者/AIDS病人的外周血的单核细胞进行扩增,获得了HIV-1膜蛋白(env)基因的核酸片段,并对其C2-C3区进行测定和分析.结果:深圳市疫情快速增长趋势明显;传播方式以性为主(46.0%)其它各种HIV传播途径均有发现;有B′、B、C、E亚型HIV-1毒株序列,各亚型间的基因离散率为6.31%、7.95%、3.09%、8.92%,推算其流行时间为6,8,3,9年,深圳流行株以E、B为主,且AIDS病人抗原性变异较高于HIV感染者.结论:监测数据可反映深圳市HIV感染流行主流,可预测流行趋势.