全文获取类型
收费全文 | 551篇 |
免费 | 58篇 |
国内免费 | 9篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 22篇 |
妇产科学 | 2篇 |
基础医学 | 103篇 |
口腔科学 | 27篇 |
临床医学 | 48篇 |
内科学 | 139篇 |
皮肤病学 | 3篇 |
神经病学 | 40篇 |
特种医学 | 100篇 |
外科学 | 31篇 |
综合类 | 4篇 |
一般理论 | 1篇 |
预防医学 | 51篇 |
药学 | 31篇 |
肿瘤学 | 14篇 |
出版年
2022年 | 3篇 |
2021年 | 4篇 |
2019年 | 5篇 |
2018年 | 9篇 |
2017年 | 6篇 |
2016年 | 4篇 |
2015年 | 7篇 |
2014年 | 8篇 |
2013年 | 9篇 |
2012年 | 6篇 |
2011年 | 8篇 |
2010年 | 6篇 |
2009年 | 14篇 |
2008年 | 16篇 |
2007年 | 26篇 |
2006年 | 20篇 |
2005年 | 18篇 |
2004年 | 23篇 |
2003年 | 16篇 |
2002年 | 15篇 |
2001年 | 14篇 |
2000年 | 16篇 |
1999年 | 13篇 |
1998年 | 17篇 |
1997年 | 15篇 |
1996年 | 17篇 |
1995年 | 19篇 |
1994年 | 16篇 |
1993年 | 12篇 |
1992年 | 15篇 |
1991年 | 13篇 |
1990年 | 16篇 |
1989年 | 19篇 |
1988年 | 20篇 |
1987年 | 32篇 |
1986年 | 18篇 |
1985年 | 25篇 |
1984年 | 10篇 |
1983年 | 14篇 |
1982年 | 12篇 |
1981年 | 8篇 |
1980年 | 11篇 |
1979年 | 7篇 |
1978年 | 4篇 |
1977年 | 5篇 |
1976年 | 6篇 |
1975年 | 4篇 |
1974年 | 4篇 |
1972年 | 2篇 |
1968年 | 2篇 |
排序方式: 共有618条查询结果,搜索用时 15 毫秒
1.
2.
3.
P-fimbriae vaccines 总被引:3,自引:0,他引:3
M. Bernice Kaack Auli Pere Timo K. Korhonen Stefan B. Svenson James A. Roberts 《Pediatric nephrology (Berlin, Germany)》1989,3(4):386-390
To test for cross-protective capacity of two different P-fimbriae vaccines we vaccinated baboons with fimbriae purified from either Escherichia coli strain ER2 or strain JR1. The vaccinated animals showed elevated antibody titers to P-fimbriae from each of the E. coli strains used, suggesting cross-reactivity as was expected from the results of immunoprecipitation of the fimbriae. Enzyme-linked immunosorbent assay inhibition by heterologous P-fimbriae proved this to be true immunologic cross-reactivity. 相似文献
4.
Anderson RA; Evans LW; Irvine DS; McIntyre MA; Groome NP; Riley SC 《Human reproduction (Oxford, England)》1998,13(12):3319-3325
Follistatin is a binding protein for the activin and inhibin family of
hormones, regulating their biological activity. In the male reproductive
tract, the interaction of these factors is likely to be involved in the
regulation of the proliferation of several cell types. We have investigated
the presence of follistatin and activin A in seminal plasma using specific
immunoassays and have localized follistatin and activin/inhibin subunits in
the adult human testis, prostate and seminal vesicle to establish their
likely sources. High concentrations of immunoreactive follistatin were
present in seminal plasma in normal men (mean 97.9 ng/ml; 1.43 ng/ml in
peripheral plasma) and were similar in men with oligo/azoospermia and
following vasectomy. Follistatin immunoreactivity was localized to both
Leydig and Sertoli cells of the testis, and to epithelial cells of the
prostate gland and seminal vesicle, which are likely to be the predominant
sources of the hormone in seminal plasma. Activin A was also present in
seminal plasma in normal men but was undetectable following vasectomy, thus
deriving from the testis. Consistent with this finding, the betaA-subunit
was immunolocalized in Sertoli and Leydig cells but was not present in
seminal vesicle or prostate gland. The functional significance of the high
concentrations of follistatin secreted into seminal plasma by the prostate
gland and/or seminal vesicle is uncertain, but they may regulate the
biological activity of testis-derived activin A and inhibin B.
相似文献
5.
6.
Technical considerations in the surgical approach to multiple accessory pathways in the Wolff-Parkinson-White syndrome 总被引:1,自引:0,他引:1
J G Selle W C Sealy J J Gallagher J M Fedor R H Svenson S H Zimmern 《The Annals of thoracic surgery》1987,43(6):579-584
Surgical techniques for the approach to and division of atrioventricular accessory pathways have been designed and perfected during the past 18 years. The standard method of exposure of a single left free wall accessory pathway is by a left atriotomy. All other single accessory pathways are exposed through a right atriotomy. Up to twenty percent of patients with Wolff-Parkinson-White (WPW) syndrome harbor multiple atrioventricular accessory pathways. In this subgroup, classic operative techniques, especially the methods of approach, must be combined or modified depending on the specific locations of the accessory pathways encountered. Eighteen of 90 patients operated on for WPW syndrome at Charlotte Memorial Hospital from August, 1983, through September, 1986, had multiple accessory pathways. Thirty-eight of thirty-nine pathways were successfully divided. One posterior septal accessory pathway reappeared 2 months postoperatively and was catheter ablated. The most frequent combination of atrioventricular accessory pathways included a right free wall and a posterior septal accessory pathway (10 patients). This combination is approached by a right atriotomy. The posterior septal space dissection is extended onto the right free wall area. Technically the most difficult combination includes a left free wall and a posterior septal accessory pathway (3 patients in the present series). Our preferred approach is begun with a right atriotomy for the posterior septal space dissection, followed by an atrial septotomy to expose the left free wall area. There are other methods, however, that may be advantageous depending on the exact locations of the accessory pathways encountered. 相似文献
7.
Expression of interleukin-1 alpha, interleukin-1 beta and interleukin-6 in chronic B lymphocytic leukaemia (B-CLL) cells from patients at different stages of disease progression. 下载免费PDF全文
M Aguilar-Santelises R Magnusson S B Svenson A Loftenius B Andersson H Mellstedt M Jondal 《Clinical and experimental immunology》1991,84(3):422-428
We have previously found that isolated B-CLL cells from progressive disease produce less interleukin-1 beta (IL-1 beta) as compared with cells from patients with indolent disease. Here we extend that finding to include measurements of IL-1 beta mRNA and secretion of IL-1 alpha and interleukin-6 (IL-6). As before, a lower production of IL-1 beta was found in cells from progressive disease. IL-6 was produced by cells from patients at all stages, with a tendency to follow the IL-1 beta production. Low secretion of IL-1 alpha was noted. When viable cells were permeabilized and analysed at the single cell level with monoclonal antibodies, most B-CLL cells were found to contain IL-1 alpha. A minor fraction of non-permeabilized cells expressed IL-1 alpha at the cell membrane. However, only small fractions of cells were positive for intracellular IL-1 beta (less than 1%) and almost no IL-6-positive cells were found. We conclude that either IL-1 beta and IL-6 are produced by a minor population of undefined cells, or that a more sensitive in situ method is needed to detect production of these cytokines in B-CLL cells. The possible biological significance of secreted, and membrane-expressed helper factors in B-CLL is discussed. 相似文献
8.
It has been recently reported that neutrophils are involved in the regulation of NK cell activity. However, the mechanism of such regulation is unclear. The present study was designed to investigate the mechanisms involved in the regulation of NK cytotoxicity by human neutrophils. The role of indomethacin, an anti-inflammatory drug, in this interaction was studied. NK cells were purified from peripheral blood obtained from normal individuals. NK cell cytotoxicity was tested on K 562 cell line by Cr release assay. Autologous neutrophils obtained from peripheral blood were stimulated by opsonized zymosan either in the presence or absence of indomethacin. The role of neutrophil supernatant containing oxygen radicals and prostaglandins on NK cytotoxicity was examined. It was shown that supernatants from stimulated neutrophils significantly inhibited (P less than 0.05) the autologous NK cell cytotoxicity. The presence of indomethacin in the in vitro reaction mixture, or given orally to donors, partially or completely abolished the inhibitory effect of neutrophil supernatant. Indomethacin inhibited prostaglandin E2 release, and luminol-enhanced, myeloperoxidase-mediated chemiluminescence of activated PMN. Diafiltration of neutrophil supernatant showed that the inhibitory activity was present in the fraction containing molecules lower than 5,000 daltons. In conclusion, our findings indicate that down-regulation of NK cytotoxicity is mediated by prostaglandins produced by stimulated neutrophils and possibly by oxygen radicals. 相似文献
9.
Molecular epidemiological studies of Mycobacterium bovis infections in humans and animals in Sweden. 下载免费PDF全文
R Szewzyk S B Svenson S E Hoffner G Blske H Wahlstrm L Englund A Engvall G Kllenius 《Journal of clinical microbiology》1995,33(12):3183-3185
Forty-nine isolates of Mycobacterium bovis from humans and animals in Sweden were analyzed by restriction fragment length polymorphism (RFLP) patterns probed by the insertion element IS6110. Most isolates had patterns indicating the presence of only one or two genomic copies of the IS6110 insertion element. This simple type of pattern was found in all human isolates. In contrast, isolates from M. bovis infections in five herds of farmed deer in Sweden showed a specific RFLP pattern with seven bands, indicating seven copies of the IS6110 sequence. In 1958, Sweden was declared free from M. bovis in cattle. However, in 1987, M. bovis was reintroduced with imported farmed deer, and since 1991, 11 outbreaks in deer herds, but not in other livestock or wildlife, have been diagnosed. Continued RFLP studies of the new Swedish M. bovis isolates can reveal possible transmission of this deer strain to other animals or humans. 相似文献
10.
Semenov SY Svenson RH Bulyshev AE Souvorov AE Nazarov AG Sizov YE Posukh VG Pavlovsky A Tatsis GP 《Annals of biomedical engineering》2000,28(1):55-60
The proposed dielectrical relaxation model of the myocardium in the microwave spectrum has been verified both on test solutions and on normal canine myocardium. Furthermore, the model was utilized to reconstruct the changes in tissue properties (including myocardial bulk resistance and water content) following myocardial acute ischemia and chronic infarction. It was shown that the reconstructed myocardial resistance and water content correlate dynamically with the process of the development of acute myocardial ischemic injury. In chronic cases the reconstructed resistance and water content of infarcted myocardium are significantly different from that of normal myocardium: the resistance is lower and water content is higher than in normal myocardium. © 2000 Biomedical Engineering Society.
PAC00: 8764-t, 8719Xx 相似文献