全文获取类型
收费全文 | 52670篇 |
免费 | 5258篇 |
国内免费 | 3718篇 |
专业分类
耳鼻咽喉 | 385篇 |
儿科学 | 685篇 |
妇产科学 | 334篇 |
基础医学 | 4532篇 |
口腔科学 | 800篇 |
临床医学 | 6384篇 |
内科学 | 6037篇 |
皮肤病学 | 383篇 |
神经病学 | 1882篇 |
特种医学 | 1941篇 |
外国民族医学 | 18篇 |
外科学 | 5325篇 |
综合类 | 12801篇 |
现状与发展 | 8篇 |
一般理论 | 1篇 |
预防医学 | 5217篇 |
眼科学 | 941篇 |
药学 | 5688篇 |
66篇 | |
中国医学 | 4397篇 |
肿瘤学 | 3821篇 |
出版年
2024年 | 229篇 |
2023年 | 728篇 |
2022年 | 1835篇 |
2021年 | 2228篇 |
2020年 | 1891篇 |
2019年 | 1351篇 |
2018年 | 1405篇 |
2017年 | 1462篇 |
2016年 | 1341篇 |
2015年 | 2127篇 |
2014年 | 2791篇 |
2013年 | 2863篇 |
2012年 | 4066篇 |
2011年 | 4322篇 |
2010年 | 3415篇 |
2009年 | 2988篇 |
2008年 | 3256篇 |
2007年 | 3443篇 |
2006年 | 3094篇 |
2005年 | 2677篇 |
2004年 | 2268篇 |
2003年 | 2485篇 |
2002年 | 1980篇 |
2001年 | 1757篇 |
2000年 | 1284篇 |
1999年 | 944篇 |
1998年 | 503篇 |
1997年 | 502篇 |
1996年 | 353篇 |
1995年 | 329篇 |
1994年 | 328篇 |
1993年 | 179篇 |
1992年 | 228篇 |
1991年 | 183篇 |
1990年 | 154篇 |
1989年 | 156篇 |
1988年 | 129篇 |
1987年 | 105篇 |
1986年 | 83篇 |
1985年 | 83篇 |
1984年 | 30篇 |
1983年 | 17篇 |
1982年 | 13篇 |
1981年 | 11篇 |
1980年 | 6篇 |
1979年 | 19篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1959年 | 1篇 |
1929年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
【摘要】 报道临床症状不典型的家族性黑棘皮病1家系。先证者女,4岁,自1周岁时,颈部、腹部出现黑色斑片,近年来逐渐扩大至唇周、躯干前部。腹部皮肤全反式共聚焦显微镜检查可见乳头环下延扭曲及沟壑结构,乳头环内可见中高折光颗粒结构。先证者父亲及祖母既往有类似病史,但随着年龄增长色素沉着自发性消退,仅有局部皮纹增粗。采集先证者及父母、祖母外周血,对先证者外周血DNA行Panel靶向测序,结果显示,先证者存在FGFR3基因14号外显子c.1949A>C(p.Lys650Thr)错义突变,Sanger测序验证证实先证者及其父亲和祖母均存在此突变。诊断:家族性黑棘皮病。 相似文献
5.
Objective: To assess the effect of electroacupuncture(EA) on expression of cytoskeletal proteins from Sertoli cells(SCs) and spermatogenesis in rats with oligozoospermia of insufficiency of Shen(Kidney)essence syndrome(OIKES).Methods: Twenty healthy male Sprague-Dawley rats were randomly assigned to four groups using a random number table: control,tripterygium glycosides(TG) treatment,sham and EA groups(n=5 in each group).A rat model of OIKES was established by oral gavage with TG.The EA group was treated with TG and received EA at Shenshu(BL 23) and Zusanli(ST 36) acupoints for 20 min,once daily for 30 days,while the sham group received EA at identical acupoints with skin penetration without stimulation.After 30 days,the ?nal body weight and coef?cients for the testis and epididymis were calculated and sperm parameters were measured.Immunohistochemical analyses were performed to detect expression of vimentin and α-tubulin in SCs and proliferating cell nuclear antigen(PCNA) immunoreactivity in germ cells.Apoptosis in germ cells was quanti?ed by the transferase biotin-dUTP nick end labeling assay.Results: Compared with the control group,the final body weight and testis/epididymis coefficients of rats in the TG-treated group were not significantly different,but the sperm count and motility were lower(P0.05).Expressions of vimentin and α-tubulin were also signi?cantly weaker(P0.01).The PCNA immunoreactivity of germ cells was decreased(P=0.059),whereas the apoptotic index of germ cells was increased signi?cantly(P0.01).In contrast,EA at BL 23 and ST 36 acupoints signi?cantly improved the ?nal body weight as well as the sperm count,concentration and motility(P0.01 or P0.05).EA increased expression of vimentin and α-tubulin in SCs markedly,and signi?cantly enhanced PCNA immunoreactivity with decreased apoptosis in germ cells(P0.01 or P0.05).Conclusions: EA at BL 23 and ST 36 acupoints has protective effects on spermatogenesis in rats with OIKES.This effect seems to be achieved by attenuating TG-induced disruption of cytoskeletal protein in SCs. 相似文献
6.
7.
沙苑子本草考证 总被引:1,自引:0,他引:1
沙苑子为我国常用中药,药用历史悠久。通过查阅本草文献,对沙苑子名称、基原、功效、产地及伪品5个方面进行考证,得知其名称演变大致经过"白蒺藜""沙苑蒺藜""沙苑子"3个阶段;历代本草记载沙苑子基原为扁茎黄芪的干燥成熟种子,古今应用品种基本一致;沙苑子补肾固精功效的记载自宋《本草衍义》起逐渐清晰明确;沙苑子道地产区自宋时起为陕西沙苑,明清时期产地逐渐转到陕西潼关、山西等地,建国后以陕西省为主向北扩散,以陕西潼关为佳;沙苑子建国前有马薸子、色微绿伪品及紫云英子等混伪品,建国后有紫云英、猪屎豆、华黄芪和直立黄芪的种子等混伪品。为沙苑子正本清源,并为其进一步深入开发提供参考。 相似文献
8.
随着现代检验医学的发展,传统医学检验正在向现代化检验医学的转变,急需加强检验与临床的沟通,医师型检验人才应运而生。目前,由于相关的管理体制,人文教育,工作职责不清晰等原因,检验医师队伍发展缓慢,仍存在很多值得改善和探索的地方。因此,我们应督促检验医师队伍,要具备较高的人文精神,掌握扎实的专业知识,参与完善的培训体制,明确工作职责,提高检验医师学习临床知识,掌握和运用医学检验知识,同时加强对临床与检验医学综合运用的能力,向临床医师提供医学检验知识,更好的与临床医生沟通解决临床问题,加强对检验医师的宣传与认识,共同促进检验医师队伍的规范化发展。 相似文献
9.
10.
Keyang Qian Hanqing Qian Juan Cai Wuheng Yue Xiaoxiao Yu Baorui Liu 《Pathology, research and practice》2019,215(4):755-760
Gastric cancer, one of the most common disease, has become a major public health problem worldwide. Cisplatin (DDP) has been a widely used drug for the treatment of cancer, also usually applied in gastric cancer in clinic. However, the side effects including toxicity and drug-resistance restricted the usage of DDP in clinic, so we prepared a DDP-complexed hydrogel (DDP-Gel) and investigated its efficacy in gastric cancer. For in vivo studies, MKN45-Luc cells were injected into BLAB/C node mice subcutaneously to establish gastric cancer with orthotopically grown tumors. Mice bearing tumors were treated with normal saline, DDP and DDP-Gel. Body weight and survival condition were observed and recorded. The treatment efficacy in vivo was detected by luciferase imaging and histological evaluation was performed by H&E staining of different organs. Additionally, normal ICR mice were treated with different doses of DDP/DDP-Gel to calculate their LD50 in vivo. The results showed that DDP-Gel prolonged survival time and ameliorated body weight changes of mice bearing tumors. DDP-Gel exhibited higher efficacy to inhibit tumor growth and metastasis, compared to DDP. Besides, LD50 of DDP-Gel was 166.0?mg/kg, 13.2 folds higher than DDP. As a conclusion, DDP-Gel showed a more effective and safer function than DDP in gastric cancer, which indicating that DDP-Gel might be a novel strategy for gastric cancer therapy. 相似文献