血液灌流对硫线磷和硫酸阿托品的吸附作用

Objective To study on adsorption effect of cadusafos and atropine sulfate by hemoperfusion.Method Hemoperfusions were performed for sheep blood samples with cadusafos and atropineby through imitated extracorporeal closed circulating perfusion apparatus.Residual cadusafos was determined by gas chromatography and residual atropine was determined by high performance liquid chromatography.Result Dose of adsorption agent was 0.5,1.0 and 1.5 g,respectively.Two hours after hemoperfusion with membrane coated activated charcoal,clearance rate of cadusafos in 3 groups all exceeded 90%.and clearance rate of atropine sulfate was 61.9%,84.9%,88.9%,respectively.One and a half hours after hemoperfusion with HA230 absorption resin,clearance rate of eadusafos in 3 groups all exceeded 90%,and clearance rate of atropine sulfate was 88.0%,97.2%,98.4%,respectively.Three hours after hemoperfusion with membrane coated activated charcoal,The concentration ratio of cadusafos and atropine sulfate in blood promoted to 10.1 times,and the ratio was 6.7 times after hemoperfusion with HA230 absorption resin.Conclusion It suggested that cadusafos were mostly removed from blood after 1.5～2.0hours hemoperfusion with membrane activated charcoal or HA230 absorption resin.The concentration ratio of cadusafos and atropine sulfate in blood will increased after hemoperfusion.     

血液灌流对硫线磷和硫酸阿托品的吸附作用

目的 定量研究血液灌流对有机磷农药硫线磷和其解毒药阿托品的吸附作用.方法 模拟临床血液灌流装置,对含硫线磷和硫酸阿托品的血样进行灌流吸附,分别用毛细管气相色谱法和高效液相色谱法测定硫线磷和硫酸阿托品的残留量.结果 吸附剂用量为0.5、1.0和1.5 g,包膜活性炭在灌流2.0 h后硫线磷的清除率均能达到90%以上,硫酸阿托品的清除率依次为61.9%、84.9%和88.9%;HA230树脂在灌流1.5 h后硫线磷清除率都达到90%以上,硫酸阿托品的清除率也依次高达88.0%、97.2%和98.4%;包膜活性炭灌流3.0h后,硫酸阿托品与硫线磷的比值最高为灌流前的10.1倍,而HA230树脂灌流后,此比值最高为灌流前的6.7倍.结论 包膜活性炭和HA230吸附树脂血液灌流1.5～2.0 h均能清除血中大部分硫线磷,而且均能增加血中硫酸阿托品和硫线磷浓度的比值.     

血液灌流对硫线磷和硫酸阿托品的吸附作用

Objective To study on adsorption effect of cadusafos and atropine sulfate by hemoperfusion.Method Hemoperfusions were performed for sheep blood samples with cadusafos and atropineby through imitated extracorporeal closed circulating perfusion apparatus.Residual cadusafos was determined by gas chromatography and residual atropine was determined by high performance liquid chromatography.Result Dose of adsorption agent was 0.5,1.0 and 1.5 g,respectively.Two hours after hemoperfusion with membrane coated activated charcoal,clearance rate of cadusafos in 3 groups all exceeded 90%.and clearance rate of atropine sulfate was 61.9%,84.9%,88.9%,respectively.One and a half hours after hemoperfusion with HA230 absorption resin,clearance rate of eadusafos in 3 groups all exceeded 90%,and clearance rate of atropine sulfate was 88.0%,97.2%,98.4%,respectively.Three hours after hemoperfusion with membrane coated activated charcoal,The concentration ratio of cadusafos and atropine sulfate in blood promoted to 10.1 times,and the ratio was 6.7 times after hemoperfusion with HA230 absorption resin.Conclusion It suggested that cadusafos were mostly removed from blood after 1.5～2.0hours hemoperfusion with membrane activated charcoal or HA230 absorption resin.The concentration ratio of cadusafos and atropine sulfate in blood will increased after hemoperfusion.     

人血清中乐果的气相色谱分析

〔目的〕建立一种简便、快速、灵敏、准确人血清中乐果的萃取净化及气相色谱分析方法。〔方法〕用丙酮提萃人血清中乐果 ,离心分离 ,用火焰光度检测的气相色谱法直接测定其含量。〔结果〕人血清中乐果的检出限为 0 .0 5ng ,低、中、高三种浓度的平均加标回收率为 89.3 %～ 10 3 .9% ,RSD为 4.5 7%～ 6.13 %。〔结论〕方法简便、快速、灵敏、准确 ,具有较好的应用价值。     

包膜活性炭血液灌流吸附甲胺磷的实验研究

目的探讨包膜活性炭对血液中甲胺磷的清除效率,并与HA230树脂比较。方法采用包膜活性炭吸附柱对中毒血样进行灌流吸附,将吸附后血样经乙酸乙酯萃取后,用毛细管气相色谱法测定其含量并计算清除率。结果分析方法的检出限为0.05ng,低、中、高三种浓度的加标回收率为92.9%～102.3%,相对标准偏差为4.92%～9.17%。灌流吸附后血中毒物的清除率为92.6%～98.3%。结论用包膜活性炭吸附剂进行血液的灌流吸附,能清除大部分毒物,迅速降低血中甲胺磷浓度。对抢救重度甲胺磷中毒的危重病人有重要价值。     

血液灌流在抢救急性中毒的临床应用

血液灌流（hemopedusion，HP）是临床常用的血液净化方法之一，通过将患者的血液从体内引出，利用体外循环灌流器中吸附剂的吸附作用，清除外源性和内源性毒物、药物、代谢产物及致病因子等，从而达到净化血液、治疗疾病之目的。HP的研究开始于1964年，但由于存在严重的血液相容性问题，从而阻碍了其临床应用。直到1970年Chang等开创性地用火棉胶白蛋白包囊活性炭，克服了直接使用活性炭引起的各种并发症，才使HP应用于临床。     

两种吸附剂血液灌流对丙溴磷的灌流清除率的影响

目的探讨包膜活性炭和HA230树脂对丙溴磷的灌流清除率,并比较其清除效果。方法分别用包膜活性炭和HA230树脂对中毒血样进行灌流吸附,将吸附后血样经乙酸乙酯萃取后,用毛细管气相色谱法检测并计算清除率。结果分析方法的检出限为0.8ng,低、中、高3种浓度的加标回收率为95.1%~101.2%,相对标准偏差为1.11%~10.97%。灌流吸附后血中丙溴磷的清除效率在包膜活性炭为78.2%~85.8%,在HA230吸附树脂为84.5%~93.8%。结论用包膜活性炭和HA230树脂吸附剂进行血液灌流,均能迅速降低血中丙溴磷浓度。     

人血清中甲基对硫磷的气相色谱测定

目的：建立一种人血清中甲基对硫磷的萃取分离及气相色谱测定方法：方法：血清经丙酮萃取后，离心分离，用火焰光度检测的气相色谱法测定甲基对硫磷的含量。结果：血清中甲基对硫磷的检出限为0．05ng，最低检出浓度为0．40μg／ml，平均加标回收率为92．1％～102．3％，相对标准偏差为4．68％～5．97％。结论：灵敏度高，且简便、快速、准确。     

油菜和桃中拉维因残留和降解规律的研究

目的 弄清田间喷施拉维因后的残留及降解规律,以便指导农民合理施药,安全采摘果蔬,减少污染,保障人们的身体健康。方法 用液－液萃取和气相色谱法分析田间喷施不同浓度拉维因后,不同天数油菜和桃中的拉维因的残留量。结果 拉维因在油菜和桃中降解符合动力学一级反应。按推荐量施药,拉维因在油菜和桃中的降解半衰期分别为７．５１ｄ、６．１７ｄ,施药后第１５天降解率分别为９０．７％、８９．２％,并且施药量与残留量呈正     

噻虫胺胁迫对小白菜中谷胱甘肽和蛋白质含量的影响

目的 寻找较敏感的生物标记物,作为噻虫胺污染蔬菜的早期诊断指标.方法 谷胱甘肽(GSH)含量测定采用Ellman法.可溶性蛋白质含量测定采用考马斯亮蓝.结果 在胁迫初期GSH含量急剧降低,低浓度处理组GSH含量在第5天下降到最低点,高浓度处理组GSH含量在第7天下降到最低点.处理组的蛋白质含量均明显低于对照组,浓度越高...