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目的:分析本市2005年不同来源01群和0139群霍乱弧菌菌株表型和ctxA毒力基因特征。方法:以PCR方法,对所有菌株进行ctxA毒力基因检测。采用WHO推荐的改良K—B纸片法,对所有菌株均进行了12种抗菌药物的药敏试验。结果:从病人和外环境中分离出的0139群霍乱弧菌均为产毒株;从病人中分离出的01群霍乱弧菌流行株均产毒,外环境中分离出的非流行株少数也产毒。0139群霍乱弧菌菌株耐药谱较01群霍乱弧菌菌株广;两者对氨苄青霉素、强力霉素、庆大霉素、复方新诺明、利福平耐药率有显著差异。结论:本市2005年霍乱的流行株都是产毒株,但产毒株不一定是流行株。药敏结果显示,对01群和0139群霍乱弧菌应采取不同抗菌治疗,0139群霍乱弧菌多重耐药应引起重视。 相似文献
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呼吸道感染病人军团菌抗体水平调查 总被引:4,自引:0,他引:4
军团菌病是近20年来新发现的传染病之一,主要由嗜肺型军团菌引起。据世界卫生组织报道,军团菌是肺部感染的重要病原体之一,其引发的肺炎占9.5%~29%。军团菌病主要有两种不同的综合征表现:一是可能致命的全身多系统的疾病,又称军团病,包括肺炎;二是自限性的类流感症状,又称Pontiac热。由于缺乏军团菌感染的临床警惕性,常常导致误诊。2002~2003年笔者对上海地区进行了军团菌血清学调查,以期了解各种人群军团菌感染状况及其主要血清型别。 相似文献
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本文报道用等导数值光谱法测定寒热痹胶囊中麻黄碱的含量。结果表明,该法操作简便、灵敏度高,重现性好。线性范围为0.08~0.48mg/ml,平均回收率为100.4%,变异系数为0.39%. 相似文献
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目的 研究上海市公共场所16个空调冷却塔水中军团菌的脉冲场凝胶电泳(PFGE)分型,并连续监测该菌基因型变化.方法 2007年5-10月连续6个月以每月1次的频率采样,从16个公共场所空调冷却塔水中分离军团菌,经血清学凝集试验、胶乳凝集试验分型后,使用PFGE技术对酶切后的军团菌全染色体DNA进行电泳获得指纹图谱,利用BioNumerics软件进行聚类分析.结果 16个冷却塔水中共分离出131株军团菌,包括嗜肺军团菌、博杰曼军团菌、米克戴德军团菌和茴香军团菌,分为52个PFGE型别,其中37个PFGE型别(71.15%)仅分布于1个冷却塔中,即为该冷却塔所特有;15个PFGE型别(28.85%)分布于2个或以上冷却塔中.16个冷却塔具有2个或以上的PFGE型,13个冷却塔(81.25%)中多次出现相同的PFGE型别.2007年6-10月连续5个月从6个冷却塔中分离出18株PFGE型为LPAs.SH0078型的军团菌.结论 冷却塔水中的军团菌基因型具有多样性和复杂性,81.25%的冷却塔水中PFGE型具有持续性,且LPAs.SH0078型广泛分布,可能为优势PFGE型. 相似文献
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Objective To investigate the genotypic characteristics and persistence of Legionella pulsed-field gel electrophoresis (PFGE) patterns in 16 air-conditioner cooling towers in six different public sites of Shanghai. Methods From May to October, continuous sampling was operated once per month in 2007. Legionella strains isolated from the 16 cooling towers were confirmed by serological and latex agglutination. PFGE was applied for the fingerprinting of the isolates, while the culster results of PFGE were analyzed by BioNumerics software. Results 131 strains of Legionella were isolated, including L. pneumophila, L. bozemanae, L. micdadei and L anisa.52 distinguishable PFGE patterns were differentiated among the 16 cooling towers, with 37 patterns were owned by just one cooling tower, which was not shared with other cooling towers, while 15 patterns were shared by more than 2 cooling towers. All the cooling towers had ≥2 PFGE patterns,while in 13 cooling towers the same PFGE patterns were recovered during the six months. From June to October of 2007, 18 strains of Legionella belonging to the PFGE pattern of LPAs. SH0078 were isolated continuously from 6 cooling towers. Conclusion This study demonstrated great genotypic diversity and complexity of Legionella in cooling towers. Persistence of the PFGE patterns was observed in 81.25% of the cooling towers. The PFGE pattern of LPAs. SH0078 was distributed widely,suggesting it might be the dominate strain in Shanghai. 相似文献
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王刚毅 《江苏大学学报(医学版)》1998,(3)
为了提高日常ELISA方法检测丙肝的精度,我们就得要对HCV检测进行质量控制。这是因为:在ELISA方法中还存在着:①酶反应的抑制性与非抑制性竞争。②免疫的特异性与非特异性的反应。③试剂厂家并未将丙肝抗原非结构区蛋白完全包被(一般包被NS3NS4NS... 相似文献