外固定架治疗桡骨远端粉碎性骨折

目的总结桡骨远端粉碎性骨折的外固定架治疗疗效。方法从1995年～2003年采用外固定架治疗桡骨远端粉碎性骨折46例,并进行随访。结果治疗后均随访1年,术后按Dienst评分,优良率为83．3％。结论外固定架固定牢靠,可早期活动腕关节,是治疗桡骨远端粉碎性、开放性骨折的有效方法。     

4种病原菌特异基因片段阳性蜱的16S rRNA基因克隆文库分析

Objective To develop the method of 16S rRNA gene clone library for tick bacterial flora analysis, and to analyze the detection effective of pathogens in tick and capacity of bacterial flora diversity. Methods Primers were designed according to the specific gene of Borrelia burgdorferi, Bartonella henselae, Anaplasma phagocytophilum, Ehrlichia chaffeensis and templates were choosen by positive PCR result to amplify the DNA extracted from the ticks. One set of primers targeting 16S rRNA gene conserved region were chosen to amplify certain fragments, DNA extraction, PCR reaction, cloning and sequencing. Nucleotide sequences were compared with GenBank database. Calculated Coverage values of clone library and Shannon-Wiener diversity index. Results Sixteen defined genus-or species-bacteria were detected in 103 valid sequences. Eight species were edge type (Clone No. > 5). Three kinds of pathogens were identified (Borrelia burgdorferi, Bartonella henselae and Rickettsia sp). Three kinds of pathogens were not edge type(Clone No. < 5). Coverage value was 96.11%, and Shannon-Wiener index was 2.40. Analysis results of cloning sequence showed that tick-parasitic bacteria mainly were α and γ deformation mycetes which accounted for 56.25% (9/16). Conclusions The 16S rRNA gene sequences technology could make relative quantitative of bacterial flora, and detect many kinds of pathogens in tick. It's a good method for detection of pathogens and bacterial flora analysis.     

4种病原菌特异基因片段阳性蜱的16S rRNA基因克隆文库分析

Objective To develop the method of 16S rRNA gene clone library for tick bacterial flora analysis, and to analyze the detection effective of pathogens in tick and capacity of bacterial flora diversity. Methods Primers were designed according to the specific gene of Borrelia burgdorferi, Bartonella henselae, Anaplasma phagocytophilum, Ehrlichia chaffeensis and templates were choosen by positive PCR result to amplify the DNA extracted from the ticks. One set of primers targeting 16S rRNA gene conserved region were chosen to amplify certain fragments, DNA extraction, PCR reaction, cloning and sequencing. Nucleotide sequences were compared with GenBank database. Calculated Coverage values of clone library and Shannon-Wiener diversity index. Results Sixteen defined genus-or species-bacteria were detected in 103 valid sequences. Eight species were edge type (Clone No. > 5). Three kinds of pathogens were identified (Borrelia burgdorferi, Bartonella henselae and Rickettsia sp). Three kinds of pathogens were not edge type(Clone No. < 5). Coverage value was 96.11%, and Shannon-Wiener index was 2.40. Analysis results of cloning sequence showed that tick-parasitic bacteria mainly were α and γ deformation mycetes which accounted for 56.25% (9/16). Conclusions The 16S rRNA gene sequences technology could make relative quantitative of bacterial flora, and detect many kinds of pathogens in tick. It's a good method for detection of pathogens and bacterial flora analysis.     

肝硬化合并多脏器衰竭的临床特点

198 5～ 1999年 ,我院共收治肝硬化失代偿期患者 5 89例 ,其中 14 5例合并多脏器衰竭 (MOF)。现报告如下。资料分析 :本组 14 5例肝硬化合并 MOF患者 ,男 90例 ,女 5 5例 ;年龄 2 3～ 65岁 ,平均 48.5岁 ;病程 2～ 2 5年。其中肝炎后肝硬化 14 2例 ,酒精性肝硬化 2例 ,胆汁性肝     

复合二氧化氯消毒饮用水副产物含量分析

目的了解复合二氧化氯消毒饮用水产生的副产物含量及其影响因素,探讨饮用水中消毒副产物含量变化规律。方法对2008-2013年复合二氧化氯消毒的出厂水和管网末梢水的亚氯酸盐、氯酸盐、三氯甲烷等指标进行检测分析。结果出厂水和管网末梢水的各项检测指标合格率以及各消毒副产物的均值差异均无统计学意义(P值均0.05);二氧化氯、耗氧量与消毒饮用水产生的副产物亚氯酸盐含量相关。结论复合二氧化氯消毒饮用水避免了有机副产物三卤甲烷污染的卫生问题,但还需提高二氧化氯转化率,并优化投加方式。     

腹腔镜下腹腔灌洗引流对重症急性胰腺炎全身炎症反应的影响

目的:探讨腹腔镜下置管腹腔灌洗引流(LPLD)对重症急性胰腺炎(SAP)全身炎症反应的影响.方法:37例SAP患者按入院先后顺序随机分为内科组和LPLD组.检测并比较两组治疗前后0、1、3、7、10 d外周血内毒素、TNF-α、IL-lβ、IL-6的浓度及单核细胞NF-κB的活性.结果:第0天 LPLD组内毒素、TNF-α、IL-lβ、IL-6浓度及单核细胞NF-κB活性与内科组比较无显著差异;术后第10天,LPLD组内毒素、TNF-α、IL-lβ、IL-6浓度及单核细胞NF-κB活性分别为(1.64±0.13)pg/mL、(114.13±15.12)pg/mL、(80.17±18.06)pg/mL、(56.43±12.24)pg/mL、(68.2±1.7)%.上述指标与内科组比较均有显著下降(P<0.01 or P<0.05).结论:LPLD能明显减轻内毒素血症,减低NF-κB活性及细胞因子浓度,有效地减轻SAP患者的炎症反应.     

中西药保留灌肠治疗溃疡性结肠炎96例

一般资料：本组男52例，女44例。年龄24～73岁，平均39．4岁，均经纤维结肠镜，乙状结肠镜或钡灌肠检查，其诊断符合1993年全国太原慢性非感染性肠道疾病学术研讨会所规定的溃疡性结肠炎诊断标准。其中，初发型38例，慢性复发型44例，慢性持续型14例，病程1～5年。治疗方法；病人确诊后，除阳其卧床休息，易消化低纤维素饮食及对症，支持治疗外，用l％奴夫卡因30ml，庆大霉素24万u，地塞米松10mg，锡类散0．6g，黄连素0．sg（研成粉末），生理盐水50ml，充分混合，加温至37C左右，嘱患者臀部抬高30cm后灌肠，半小时后臀部放平，侧卧休息，…     

卫生防疫机构在创建国家卫生城市工作中的作用

国家卫生城市称号．是一个城市的生活环境、文明程度和居民素质全面达到国家卫生城市标准的重要标志，它对于优化环境、改善人民居住生活条件具有现实意义。为此，各级政府都把创建国家卫生城市作为一项造福人民的工程，列入了工作日程。在这项工作中．卫生防疫机构承担着繁重的任务．如何在创建卫生城工作中利用自己的优势，充分发挥作用．是目前形势下值得探讨的问题。     

前路病灶清除植骨Ⅰ期内固定治疗胸腰椎结核

目的探讨前路病灶清除、Ⅰ期植骨内固定治疗脊柱结核的临床效果。方法采用一期前路病灶清除、植骨、钉棒内固定治疗胸腰椎结核39例,观察术后畸形纠正情况、植骨融合效果和治愈率。结果平均随访1年,内固定无松动、断裂,植骨融合良好,切口均一期愈合。结论前路病灶清除植骨一期内固定治疗胸腰椎结核能重建脊柱的稳定性、矫正脊柱畸形,有利于结核病灶的修复,提高治愈率。     

门脉高压性胃病120例临床观察

我院从 1985～ 1999年收治经纤维胃镜 ,Ｂ超证实肝硬化门脉高压致上消化道出血的病人 5 5 6例。对其中纤维胃镜确诊胃黏膜病变致上消化道出血的 12 0例进行了临床观察 ,并进行了回顾性分析如下。1　临床资料12 0例中男 80例 ,女 40例 ,男∶女 =2∶1;平均 48 6岁 (2 8～ 75岁 ) ,45～ 5 5岁发病率最高 ,发病前有服药史 5 0例 ,占41 7% ;有饮酒史 30例 ,占 2 5 %。临床表现呕血、黑便 ,或二者兼有。入院 2 4～ 48小时行急诊胃镜检查 ,见轻、中度食管静脉曲张 ,未见破裂迹象。 70例可见轻度门静脉高压性胃病的胃黏膜病变。①细微粉红色斑点或猩…