Apatinib induces apoptosis of breast cancer cells by redistribution of Fas into lipid rafts

Apatinib, an oral anti-angiogenic agent, has been shown to have anti-cancer effects for several cancer cell types. However, little is known about the direct anti-tumor activity of apatinib for breast cancer cells. Herein, the direct effect of apatinib on breast cancer cells and its mechanism of action were assessed. Cell viability was measured with a Cell Counting Kit-8. Cell apoptosis was assessed by flow cytometry. The expression of caspase-8 and the cleavage of poly ADP ribose polymerase were assessed by Western blotting analysis. Lipid rafts and Fas distribution were determined by immunofluorescence microscopy. Apatinib suppressed breast cancer cell proliferation in a dose-dependent manner. Furthermore, apatinib enhanced the aggregation of lipid rafts and the redistribution of Fas into lipid rafts. Pretreatment with methyl-β-cyclodextrin, a cholesterol-sequestering agent, significantlyreversed Fas redistribution and apatinib-induced apoptosis.In conclusion, these results demonstrated that apatinib induced apoptosis of breast cancer cells partially through recruitment of Fas into lipid rafts.     

对氧磷酶1与呼吸系统疾病关系的研究进展

对氧磷酶1是一种钙依赖糖蛋白,主要生理作用是防止低密度脂蛋白和高密度脂蛋白氧化修饰,抗动脉粥样硬化,此外还具有对抗细菌内毒素毒性以及对有机磷毒性的保护等作用。本文将对对氧磷酶1的结构、功能、生物特性及其与呼吸系统疾病之间的关系作一综述。     

Enhanced Photo-Fenton Activity of SnO2/α-Fe2O3 Composites Prepared by a Two-Step Solvothermal Method

The x-SnO₂/α-Fe₂O₃ (x = 0.04, 0.07, and 0.1) heterogeneous composites were successfully prepared via a two-step solvothermal method. These composites were systematically characterized by the X-ray diffraction technique, field emission scanning electron microscopy, an energy dispersive spectrometer, X-ray photoelectron spectroscopy and a UV–visible spectrometer. It was found that SnO₂ nanoparticles were uniformly decorated on the surface of α-Fe₂O₃ particles in these heterogeneous composites. A comparative study of methylene blue (MB) photodegradation by α-Fe₂O₃ and x-SnO₂/α-Fe₂O₃ composites was carried out. All x-SnO₂/α-Fe₂O₃ composites showed higher MB photodegradation efficiency than α-Fe₂O₃. When x = 0.07, the MB photodegradation efficiency can reach 97% in 60 min. Meanwhile, the first-order kinetic studies demonstrated that the optimal rate constant of 0.07-SnO₂/α-Fe₂O₃ composite was 0.0537 min⁻¹, while that of pure α-Fe₂O₃ was only 0.0191 min⁻¹. The catalytic mechanism of MB photodegradation by SnO₂/α-Fe₂O₃ was examined. The SnO₂ can act as a sink and help the effective transfer of photo-generated electrons for decomposing hydrogen peroxide (H₂O₂) into active radicals. This work can provide a new insight into the catalytic mechanism of the photo-Fenton process.     

不稳定心绞痛患者不同中医证候的血浆FIB水平回顾性分析

目的探讨血浆纤维蛋白原(FIB)水平与不稳定心绞痛(UA)的不同中医证型的对应关系。方法收集166例不稳定心绞痛患者和36例健康对照者的临床资料,对患者的血浆FIB水平及所属的中医证型进行回顾性分析。结果不稳定心绞痛组FIB水平明显高于对照组(P<0.01)。不稳定心绞痛患者中,血瘀实证的FIB水平高于血瘀证兼夹虚证组(P<0.05);气滞血瘀证的FIB水平高于气虚血瘀证组(P<0.05)。结论不稳定心绞痛血瘀证与FIB水平存在着相关性。     

高职高专辅导员工作刍议

辅导员的主要工作是管学生,做学生的思想政治工作。管人是件很难的事情,因为人心叵测,同时又在不断发展变化。人的内心世界可能一半是天使,一半是魔鬼,但人的主观愿望总是在努力适应现实环境。人在好的环境就变好,在坏的环境就变坏。只要形成好的环境,就能引导人做出良好的行为。在务虚     

重组人干扰素α-2b体外抗呼吸道合胞病毒作用的实验研究

目的观察重组人干扰素α-2b在体外的抗呼吸道合胞病毒作用。方法观察不同浓度重组人干扰素α-2b抑制呼吸道合胞病毒对Hela细胞产生细胞病变（CPE）的作用,并与利巴韦林比较。结果实验表明重组人干扰素α-2b抗呼吸道合胞病毒的最低有效浓度为25IU／ml。结论重组人干扰素α-2b具有明显的抗呼吸道合胞病毒作用。