白细胞介素1β、白细胞介素6、肿瘤坏死因子α在肝癌大鼠模型肝硬化期的变化

目的探讨免疫细胞因子白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)在肝癌大鼠模型肝硬化期的变化及作用。方法选取雄性Wistar大鼠70只,随机分为两组,正常组(35只):饮用灭菌食用水;实验组(35只):饮用灭菌食用水配制浓度0.1mg/mL的二乙基亚硝胺(DEN)溶液,分别于第5、7、9、11w检测两组大鼠外周血IL-1β、IL-6和TNF-α等免疫指标的变化。结果与正常组相比,实验组大鼠血清IL-1β、IL-6和TNF-α水平均升高,差异均具有统计学意义(P<0.01)。结论肝癌大鼠模型肝硬化期免疫细胞因子IL-1β、IL-6和TNF-α的异常变化可促进肝硬化进一步发展为肝癌。     

恶性肿瘤维医证型与血栓前状态分子标志物的关系研究

目的 探讨恶性肿瘤患者维医证型与血栓前状态分子标志物的关系。     

脊柱结核的手术治疗研究进展

脊柱结核是最常见的肺外结核,约占骨关节结核的50%遥近年来随着全球范围内结核发病率的上升,脊柱结核的发病人 数也在逐年增加遥随着对脊柱结核认识的不断深入以及外科技术的不断提高,近年来脊柱结核的治疗发展迅速,尤其是外科治 疗取得很大进步遥目前,在规范抗结核药物治疗的前提下,手术治疗脊柱结核取得了良好的效果遥     

异常黑胆质成熟剂对异常黑胆质载体动物模型下丘脑-垂体-肾上腺轴细胞超微结构的影响

目的：观察异常黑胆质成熟剂对异常黑胆质载体动物模型下丘脑、垂体、肾上腺组织细胞超微结构的影响，探索异常黑胆质成熟剂可能的作用机制。方法：采用干寒饲养环境，干寒属性的饲料饲养，慢性间断足底电击等多因素复制小鼠异常黑胆质载体动物模型，分别用异常黑胆质成熟剂小剂量2.53g/kg（生药量8.58g/kg）,中剂量5.06g/kg（生药量17.16g/kg），大剂量10.12g/kg（生药量34.32g/kg）对模型进行全程干预，透射电镜下观察其下丘脑、垂体、肾上腺细胞超微结构的变化。结果：与模型组相比,异常黑胆质成熟剂中剂量组和大剂量组下丘脑、垂体细胞神经胶质细胞内分泌颗粒增多，神经突触以及突触前部的分泌小泡明显增多，细胞器较丰富，细胞核趋于正常，胶质细胞核周隙增宽程度以及细胞内线粒体空泡样变,粗面内质网扩张程度较模型组明显减轻。小剂量组小鼠肾上腺上皮细胞核形不规则,核周隙明显增宽，异染色质较密集，脂滴明显增多，细胞内线粒体较丰富,中剂量组和大剂量组除核周隙增宽外,其余趋向正常。结论：异常黑胆质成熟剂能明显减轻下丘脑-垂体-肾上腺轴（HPA）的多个环节超微结构的病理改变，保护异常黑胆质对HPA轴的损伤。     

Distribution of human papillomavirus (HPV) genotypes detected by routine pap smear in uyghur‐muslim women from Karasay Township Hotan (Xinjiang,China)

HPV infection is an important public health problem in developing countries. We investigated HPV genotypes in the Uyghur female population of Karasay Township, Hotan region. A population‐based cervical cancer screening was conducted for 4,500 women in Karasay Township, Xinjiang Hotan, China. A total of 900 women were selected by systematic sampling with a 5:1 proportion (ages 20–69). The subjects completed a questionnaire and consented to HPV typing and Pap smear examination. Colposcopic biopsies were performed for patients with cytological abnormalities (≥ASCUS). A total of 117 of the 900 women (13%) assessed were infected with HPV. The most common subtype was HPV‐16, and other common high‐risk types included HPV‐58 and HPV‐39. A total of 40 women (4.44%) were identified with abnormal cytology (≥ASCUS) by Pap smear. A significant link was found between HPV prevalence and cytological diagnosis. The HPV infection rates for the patients with cervical inflammation, CIN, and cancer were 18.18%, 64.71%, and 100%, respectively. Significant differences in HPV infection rates were found among the patients with the three groups of pathological results. In Karasay, the HPV infection rate in Uyghur women is lower than previously reported; however, the proportion infected with HR‐HPV is higher. HPV‐16, HPV‐58, and HPV‐39 are the most prevalent genotypes. J. Med. Virol. 87:1960–1965, 2015. © 2015 The Authors. Journal of Medical Virology Published by Wiley Periodicals, Inc.     

联苯二胺类与噻吩共聚物的金属配合物催化法合成

联苯二胺，3，3′-二甲基联苯二胺在二价镍配合物存在下，直接与2，5-二溴噻吩的格氏(Grignard)试剂共聚，用红外光谱、核磁共振等对共聚物进行了表征。该合成方法所得联苯二胺与噻吩共聚物、3，3′-二甲基联苯二胺与噻吩共聚物的收率分别为63．4％和70.8％。在25℃测得的特性粘度分别为O．75dL／g和O.67dL／g。聚合物的循环伏安测定表明该类聚合物具有一定的电化学活性，每种聚合物均在O～O．8V之问出现两对氧化一还原峰。紫外一可见吸收光谱测试结果表明共聚物分别在415nm和450nm处有最大吸收峰。     

晶状体溶解性青光眼改良三联术

目的:观察前房冲吸、白内障囊外摘除及人工晶状体植入术应用于晶状体溶解性青光眼的临床效果。方法:14例(14眼)晶状体溶解性青光眼施行前房冲吸、白内障囊外摘除及人工晶状体植入术,术后门诊随访1mo观察效果。结果:本组患者13例(93%)视力达0.3以上;眼压均控制在9～19mmHg。结论:此术式简便易行、效果佳,值得推广。     

异常黑胆质成熟剂对异常黑胆质载体动物模型支配器官形态学的影响

目的:观察异常黑胆质成熟剂对异常黑胆质载体动物模型支配器官形态学的影响,从组织病理学角度探讨异常黑胆质证的本质,阐明异常黑胆质成熟剂可能的作用机理。方法:采用多因素复制小鼠异常黑胆质载体动物模型,分别用异常黑胆质成熟剂小剂量2.53 g.kg-1,中剂量5.06 g.kg-1,大剂量10.12 g.kg-1对模型进行全程干预。取脑、心脏、肝脏支配器官,观察和比较异常黑胆质载体动物模型组和药物干预组支配器官的组织形态学改变。结果:与模型组相比,异常黑胆质成熟剂小剂量和中剂量组支配器官(脑、心脏、肝脏)细胞修复性指标增强,而损伤性组织病理学改变减轻;大剂量组修复性指标明显提高,损伤性组织病理学改变显著减轻。结论:异常黑胆质成熟剂对异常黑胆质载体动物模型的支配器官组织病理学改变有一定的保护和修复作用。