Increased P-glycoprotein function and level after long-term exposure of four antiepileptic drugs to rat brain microvascular endothelial cells in vitro

To investigate whether long-term exposure to four typical antiepileptic drugs (AEDs): phenobarbital (PB), phenytoin (PHT), carbamazepine (CBZ) and valproic acid (VPA) can increase P-glycoprotein (P-gp) level and function in primary cultured rat brain microvascular endothelial cells (rBMECs) in vitro, the rBMECs were incubated in culture medium containing indicated drugs (PB, PHT, CBZ, VPA and rifampin) for 60 days in a gradient concentration manner. Age-matched cells were incubated in normal culture medium. After a 60-day exposure to the indicated drugs, P-gp function and level in cells were measured using rhodamine 123 (Rho123) accumulations and Western blot analysis, respectively. Lower Rho123 accumulation in drug-treated cells was found than that in age-matched cells. Cyclosporin A (CsA) and verapamil (Ver) increased Rho123 accumulation both in drug-treated cells and age-matched cells. The magnitude of increased Rho123 accumulation in drug-treated cells was larger than that in age-matched cells. Higher P-gp levels were found to be consistent with decrease of Rho123 accumulation in drug-treated cells. The results verified the hypothesis that long-term exposure to the four antiepileptic drugs can induce P-gp function and level in rBMECs.     

漂白剂对牙釉质和树脂黏结强度的影响

目的:比较漂白后不同时间黏结操作时,釉质与复合树脂黏结后的剪切强度,评价漂白对黏结的影响。方法:18颗人磨牙切取釉质共36片,按照随机数字表法随机分为4组。1组:漂白2周+即刻黏结;2组:漂白2周+延迟7d黏结;3组:漂白2周+延迟14d黏结;4组:生理盐水2周+即刻黏结。万能材料试验机测量剪切强度。结果:对照组为(24.4±3.7)MPa,漂白后即刻黏结,釉质剪切强度为(14.2±2.5)MPa,与漂白前有明显差异(P=0.000,95%CI7.6344～12.8478)。7d后剪切强度有所恢复,为(19.0±2.0)MPa,但与漂白前仍有差异(P=0.000,95%CI2.8322～8.0456),约14d后,剪切强度可恢复至漂白前水平,为(21.9±2.5)MPa(P=0.056,95%CI-7.0031～5.1434)。结论:漂白后即刻黏结,牙齿与树脂的黏结强度会降低。延迟黏结时间2周,黏结强度可以恢复。     

尿沉渣中钙信号相关mRNA作为IgA肾病足细胞损伤无创性指标的研究

<正>IgA肾病(IgA nephropathy,IgAN)是目前最常见的原发性慢性肾小球疾病,5%～25%的IgAN患者10年内进展为终末期肾脏病(end-stage renal disease,ESRD),需行肾脏替代治疗^[1]。近年来,足细胞在IgAN中,致病作用受到更多关注,足细胞损伤与蛋白尿及IgAN病情进展正相关^[2]。     

D-二聚体阳性患者在临床科室的分布及其检测临床意义

目的探讨D-二聚体阳性患者在临床各科室的分布及其临床意义。方法选取该院收治的1 003例患者,采用胶乳增强免疫比浊法对其血浆D-二聚体水平进行检测。结果 ICU科、心内科、呼吸内科、骨科、普外科、肝病科、神经外科、妇产科和肿瘤科患者总阳性率为44.1%。同时,弥散性血管内凝血(DIC)、深静脉血栓、肺动脉栓塞、心脑血管疾病、肝脏疾病和恶性肿瘤患者中的D-二聚体阳性者分别为86、34、26、24、18、12例。结论血浆D-二聚体水平的检测可用于血栓性疾病的预防、监控。     

人类口腔黏膜上皮细胞提取基因组 DNA 方法的初步研究

目的：探索从人类口腔黏膜上皮细胞中提取基因组 DNA 方法的可行性。方法分别采用煮沸法和碱裂解法从人口腔黏膜上皮细胞提取基因组 DNA ,然后与临床常用的碘化钠（NaI）法提取的人全血基因组 DNA 进行比较。结果煮沸法和碱裂解法2种方法均能从口腔黏膜上皮细胞抽提到 DNA ,提取的 DNA 浓度虽低于 NaI 法,但用2种方法提取到的 DNA 样品进行聚合酶链反应,均能获得满意效果,与常规 NaI 法无明显差异。结论从人口腔黏膜上皮细胞提取基因组 DNA 简便、快捷、无损,在临床上具有潜在的应用价值。