The Long-Term Effective Rate of Different Branches of Idiopathic Trigeminal Neuralgia After Single Radiofrequency Thermocoagulation: A Cohort Study

To evaluate the efficacy of computed tomography (CT) guided single radiofrequency thermocoagualtion (RFT) in 1137 patients with idiopathic trigeminal neuralgia after a follow-up period of 11 years, specially focused on duration of pain relief in different branches of trigeminal nerve, side effect, and complications.Retrospective study of patients with idiopathic trigeminal neuralgia treated with a single CT guided RFT procedure between January 2002 and December 2013.The mean follow-up time was 46.14 ± 30.91 months. Immediate postprocedure pain relief was 98.4%. V2 division obtained the best pain relief rate: 91%, 89%, 80%, 72%, 60%, and 54% at 1, 3, 5, 7, 9, and 11 years, respectively. No statistical difference pairwise comparison was in other groups. The complications included masseter muscle weakness, corneitis, diplopia, ptosis, hearing loss, limited mouth opening, and low pressure headache. Masticatory weakness mostly occurred in patients with V3 branch involvement, while Corneitis and Diplopia all in patients with V1 branch involvement. No mortalities observed during or after RFT.All different branches division of trigeminal neuralgia achieved comparable satisfactory curative effect; V2 obtained the best excellent pain relief, after RFT procedure. Facial numbness is inevitable after RFT, which patients who have pain in all 3 trigeminal divisions and patients who desire no facial numbness should be cautious. Masticatory weakness is mainly related with V3 injured, while Corneitis and Diplopia in patients with V1 injured by RFT.     

大鼠头颅瞬间侧向旋转后脑干水肿的形成及其超微形态学基础

目的：探讨头颅瞬间旋转后脑干水肿的形成及超微形态学基础。     

hCG/ATP-induced Leydig cell calcium channel reaction at different extracellular calcium ion concentration

Objective: To study the calcium channel reaction of human Leydig cells induced by hCG/ATP at different extracellular calcium ion concentrations. Methods: The Leydig cell calcium ion concentration was examined with laser confocal microscope, when the cells were stimulated with hCG/ATP at different extracellular calcium contrations. Results: With calcium-containing extracellular fluid, the Leydig cells were sensitive to hCG stimulation and when the extracellular fluid was calcium-free, the Leydig cells did not respond to the stimulation. However, the Leydig cells did respond to ATP stimulation no matter the extracellular fluid contained calcium or not. Conclusion: In human Leydig cells, there are calcium channels sensitive to hCG and ATP. The extracellular calcium ion concentration plays an important role in the regulation of Leydig cell metabolism by hCG/ATP.     

颅咽管瘤84例临床治疗评价

研究颅咽管瘤术后复发相关因素及生存质量。方法回顾性分析颅咽管瘤８４例各种治疗方式及其临床病理与预后的关系。结果复发１９例（２３％）,其中全切１４例,复发２例（１４％）,次全切１０例,复发６例（６０．０％）,次全切加放疗３５例,复发３例（９％）,大部切除加放疗１０例,复发２例（２０％）。全切及次全切加放疗术优良率均高于其它几种治疗方式,结论颅咽管瘤术后复发与其病理性质,治疗方式等因素有一定相关性。     

构建基于小波熵的自训练半监督支持向量机分类模型评价老年人步态

研究应用半监督学习算法分析未标注步态数据评价老年人步态,提出基于小波熵的自训练半监督支持向量机步态分类模型,通过小波熵从未标注步态数据中选取为每次自训练步态分类模型所需最具信息量的标注样本,有效获取步态数据类别间和步态数据内在的“有价值”的步态变异信息,提高步态分类器的泛化性能.首先采用10名老年人和10名青年人步态数据构建支持向量机分类模型,然后对120名不同年龄组未标注步态数据分类预测,依据小波熵选取样本数据,逐步添加更新步态样本训练集,自训练支持向量机分类模型.实验结果表明,本算法较准确鉴别青年和老年人步态模式(分类正确率90％),比基于有监督学习的支持向量机步态分类算法正确率提高近5％,有效改善支持向量机步态分类算法性能,有望为临床提供一个评价老年人步态的新方法.     

Systematic screen with kinases inhibitors reveals kinases play distinct roles in growth of osteoprogenitor cells

Cancer treatment-related bone loss has become growing problematic, especially in breast and prostate cancer treated with hormone/endocrine therapy, chemotherapy and radiotherapy. However, bone loss caused by targeted therapy in cancer patients is largely unknown yet. In present study, a kinase inhibitors screen was applied for MC3T3-E1, a murine osteoprogenitor cell line, and seven kinase inhibitors (GSK1838705A, PF-04691502, Dasatinib, Masitinib, GDC-0941, XL880 and Everolimus) were found to suppress the cell viability with dose- and time-dependent manner. The most interesting is that many kinase inhibitors (such as lapatinib, erlotinib and sunitinib) can promote MC3T3-E1 cell proliferation at 0.01 μM. 4 out of 7 inhibitors were selected to perform the functional study and found that they lead to cell cycle dysregulation, treatments of PF-04691502 (AKT inhibitor), Dasatinib (Src inhibitor) and Everolimus (mTOR inhibitor) lead to G1 arrest of MC3T3-E1 cells via downregulation of cyclin D1 and p-AKT, whereas XL880 (MET and VEGFR inhibitor) treatment results in increase of sub-G1 and G2/M phase by upregulation of p53 protein. Our work provides important indications for the comprehensive care of cancer patients treated with some targeted drugs.     

血管紧张素Ⅱ对大鼠胰岛β细胞形态功能的影响

目的:探讨缓慢输注血管紧张素Ⅱ(AngⅡ)后,大鼠胰岛β细胞功能和形态的变化. 方法:给予SD大鼠皮下缓慢输注AngⅡ 2 wk,成模后再分别给予正常盐(8 g/L)和高盐(40 g/L)饮食,动态观察血压、血糖、血胰岛素变化,并利用免疫组化的方法了解胰岛β细胞形态的变化. 结果:AngⅡ输注后2 wk,大鼠血压明显升高;停止AngⅡ输注后4 wk,实验组血压继续升高,实验 高盐组血压升高更明显,实验 高盐组β细胞面积明显大于对照组(P<0.05); 停止AngⅡ输注后12 wk,实验组血压比对照组血压仍高,血胰岛素均明显降低,胰岛β细胞面积明显增大(P<0.05),实验 高盐组变化更明显. 结论:缓慢输注AngⅡ可引起血压明显升高,可能影响胰岛β细胞结构,导致β细胞胰岛素分泌功能受损.     

新鲜羊膜移植治疗兔角膜碱烧伤的光镜和电镜观察

目的观察新鲜羊膜移植治疗兔角膜碱烧伤的组织学变化。方法21只（42眼）新西兰大白兔制作角膜碱烧伤模型；15只（30眼）为实验组，6只（12眼）为对照组；实验组行新鲜羊膜移植术，对照组不处理。分别取术后第1周、第2周、第4周的角膜进行光镜及电镜观察。结果术后第1周、第2周及第4周观察发现，对照组眼表面及角膜基质中有较多的炎性细胞浸润，上皮形状不规则，排列不整齐，第4周表现最明显；实验组炎性细胞浸润较少，上皮排列较整齐，显示新鲜羊膜有较强的抑制炎症并促进上皮生长的作用。通过透射电镜观察，发现新鲜羊膜移植组角膜上皮生长良好、表面有丰富的微绒毛、胶原纤维排列整齐，细胞间桥粒连接较多；对照组无上皮生长，胶原纤维排列紊乱。结论新鲜羊膜移植术在角膜碱烧伤治疗中有抑制炎症反应、促进上皮生长的作用，能够有效地促进角膜上皮的愈合及细胞之间的紧密连接。     

206例垂体腺瘤的免疫组化,电镜及内分泌特征的研究

本文采用免疫组化及电镜观测分析了206例垂体腺瘤,根据激素分泌及超微结构特征将肿瘤分为11类.其中泌乳素腺瘤最常见(35.92%);其次是生长激素腺瘤(19.42%);零细胞腺瘤(16.50%)等.本文借助流式细胞术DNA分析研究了38例术后复发的垂体腺瘤后认为:嗜酸性干细胞腺瘤和伴Nelson综合征的促肾上腺皮质激素腺瘤有术后复发的倾向;此外,侵袭性生长的腺瘤和流式细胞术测定时DNA含量较高的DNA异倍体腺瘤的恶性度相对较高,术后易于复发.