一次手术法对自制两段式种植体种植效果的实验研究

本实验用自制纯钛两段式种植体,以一次手术法植入到狗的下颌前磨牙区,观察5个月,结果表明,观察期间内牙龈无炎症,种植体稳固,X线片显示种植体周围无透射区,镜下可见种植体表面与牙龈之间形成上皮附着样结构,与骨组织之间大多呈骨性愈合。提示了两段式种植体有两种设计和手术方式可供选择。骨内部分在开放的环境中也可达到骨性愈合。     

骨髓间充质干细胞的分离培养及生物学特性研究

目的：通过体外细胞的培养方法将骨髓间充质干细胞由骨髓血中分离出来并加以纯化，进一步在体外培养条件下研究其增殖及生长特征，从而为探讨后续利用组织工程学方法修复肌肉骨骼系统组织缺损的可能性提供实验基础。方法：从骨髓血中提取间充质干细胞，体外培养扩增，再以相差显微镜、电镜观察并绘制生长曲线。结果：（1）通过离体培养，可以使体内环境下低丰度的骨髓间充质干细胞实现数目扩增。（2）体外培养条件下骨髓间充质干细胞具有成纤维细胞的生长特性。（3）体外单层培养条件下贴壁生长的骨髓间充质干细胞会出现“返祖现象”。（4）骨髓间充质干细胞内细胞器与其生物活性变化相一致。结论：骨髓间充质干细胞体外培养成功为今后利用自体间充质干细胞通过组织工程学方法修复肌肉骨骼系统的组织缺损提供功能细胞奠定了基础。     

股骨干骨折内固定治疗并发症分析

目的提高股骨骨折手术成功的几率,减少并发症的发生.方法临床上股骨干骨折术后出现并发症的29例病人进行分析,包括关节僵硬,感染,骨延迟愈合、骨不连,断钉、断板、弯曲松动退出、畸形愈合短缩等,采取不同方法进行积极二次治疗.结果 29例病人经二次治疗,临床均获治愈,经过6月~3年的观察,无再次并发症发生.结论骨折断端的血运保护、可靠固定如交锁钉、一期植骨是防止股骨干骨折并发症的重要措施.     

血塞通注射液对沙土鼠脑缺血损伤的保护作用

通过夹闭沙土鼠双侧颈总动脉１０ ｍｉｎ 或２０ ｍｉｎ 后再灌７ ｄ 或１ ｄ , 造成脑缺血再灌模型, 检测血塞通对沙土鼠脑组织钙、水含量的影响, 以及对海马 Ｃ Ａ１ 区死亡的神经元迟发性损伤的保护作用． 结果显示, 血塞通可降低缺血后脑组织钙的含量, 减少海马 Ｃ Ａ１ 区死亡的神经元数量, 增加神经元密度． 实验结果提示血塞通对脑缺血再灌损伤有一定的保护作用．     

单硝酸异山梨酯药动学和药效学特点及机制探讨

目的：对单硝酸异山梨酯药动学和药效学作用特点及其机制等方面进行评价,为该类药物的开发利用提供理论参考。方法：收集有关该药的国内外近期资料加以综合归纳。结果：较为系统的阐明了该药作用及作用机制在目前国内外研究状况,并提出将来的研究动态。结论：单硝酸异山梨酯具有疗效肯定、用途广泛、毒副作用小等特点     

掌侧切口治疗桡骨远端骨折

目的 探讨桡骨远端掌侧切口的解剖特点及手术治疗。方法经掌侧切口采用6～8孔钢板或T形钢板内固定及植骨术，5例陈旧性不愈合者，一期取髂骨植骨。结果51例患者经切开复位及植骨术后，无需外固定，术后无骨折不愈合及延迟愈合，腕关节功能恢复满意。优良率为95．2％。结论桡骨远端掌面宽大而平坦，放置钢板容易，稳定，采取掌侧切口符合生理弧度，具有较好治疗效果。     

Nociceptin/orphanin FQ up‐regulates P2X3 receptors in primary cultures of neonatal rat trigeminal ganglion neurons

Recent evidence indicates a nociceptive role of the nociceptin/orphanin FQ–opioid receptor‐like receptor (N/OFQ‐ORL1) system in craniofacial pain; however, the mechanisms of such an effect remain unclear. We investigated whether the action of N/OFQ involves the modulation of P2X₃, a pain‐transducing ionotropic receptor. Double‐labeled immunohistochemical staining was used to determine the co‐localization of ORL1 and P2X₃ receptors in the trigeminal ganglia (TG) of neonatal Sprague‐Dawley rats. The effect of N/OFQ (at a concentration ranging from 1 pM to 10 nM) on the expression of P2X₃ receptors was detected by RT‐PCR, western blotting, and immunocytochemical staining. We found that ORL1 receptors were co‐localized with P2X₃ receptors and that the application of N/OFQ could up‐regulate, in a concentration‐dependent manner, the expression of P2X₃ receptor mRNA and P2X₃ receptor protein in TG neurons. Immunocytochemical staining also revealed enhanced P2X₃ immunoreactivity beneath the neuronal membrane and an increased percentage of P2X₃‐positive neurons after treatment with N/OFQ. Those effects were completely blocked by a specific ORL1 antagonist, UFP‐101. Our results suggest that the activation of ORL1 receptors by N/OFQ can potentiate P2X₃ receptors in primary cultures of neonatal trigeminal neurons, which may be a mechanism for the nociceptive role of N/OFQ in the modulation of craniofacial pain. Our findings may also have implications in treating craniofacial pain.     

Heat Conduction in a Functionally Graded Plate Subjected to Finite Cooling/Heating Rates: An Asymptotic Solution

This work investigates transient heat conduction in a functionally graded plate (FGM plate) subjected to gradual cooling/heating at its boundaries. The thermal properties of the FGM are assumed to be continuous and piecewise differentiable functions of the coordinate in the plate thickness direction. A linear ramp function describes the cooling/heating rates at the plate boundaries. A multi-layered material model and Laplace transform are employed to obtain the transformed temperatures at the interfaces between the layers. An asymptotic analysis and an integration technique are then used to obtain a closed form asymptotic solution of the temperature field in the FGM plate for short times. The thermal stress intensity factor (TSIF) for an edge crack in the FGM plate calculated based on the asymptotic temperature solution shows that the asymptotic solution can capture the peak TSIFs under the finite cooling rate conditions.     

IRF5通过对巨噬细胞极化的调节影响脂质摄取及排出的探究

动脉粥样硬化（AS）是严重危害人类生命健康的一类慢性血管疾病,局部胆固醇的大量蓄积和炎症的发生是其主要原因。现已证明许多细胞因子通过调节巨噬细胞向M1及M2型的转化,影响巨噬细胞对脂质的摄取及排出。该文将阐述干扰素调节因子5（IRF5）是否通过调节巨噬细胞的极化而对巨噬细胞的脂质摄取及排出产生影响,从而为以IRF5为作用靶点治疗AS的可能性提供一些前期理论研究。