血清镁测定方法的比较

目的　了解临床常用的血清镁测定方法的性能。方法　对 4种常用的测定法做了精密度、线性、抗脂血能力等实验并进行了比较。结果　二甲苯胺兰法和偶氮氯磷Ⅰ法的精密度分别为CV批内 =2 .17%、CV批间 =3.4 0 %和CV批内 =2 .18%、CV批间 =3.4 %、线性达 3.0mmol/L、试剂稳定性优于甲基麝香草酚兰法和Calmagite染料法。甲基麝香草酚兰法和Calmagite染料法精密度分别为CV批内 =2 .38%、CV批间 =3.6 5 %和CV批内 =2 .38%、CV批间 =3.6 1%、线性达 2 .5mmol/L。扣除样本空白的甲基麝香草酚兰法和Calmagite染料法抗三酰甘油的能力优于二甲苯胺兰法和偶氮氯磷Ⅰ法。 4种镁测定方法的准确度较好 (偏倚 <5 % ) ,平均回收率都在95 %～ 10 5 %。 4种镁测定方法的结果相关性好 (r均 >0 .995 ) ,而灵敏度以偶氮氯磷Ⅰ法最高。结论　偶氮氯磷Ⅰ法测血清镁较适于临床使用。     

全自动生化仪使用中项目间交叉污染的探讨

目的 揭示全自动生化仪使用中项目间交叉污染的问题。方法 以无机磷、镁和三酰甘油为对象，将内含磷酸盐、镁离子或甘油类组分的检测项目和这3个项目做几种组合，观察这些试剂对病人标本检测这3个项目的影响。结果 说明这3个项目的检测设置对检测结果有明显的影响，差异达数倍。结论 对这些分析项目的设置不能随意，必须注意位置和程序的设置。     

梅毒非特异性类脂质反应素抗体试验操作的改进及应用评价

Objective To estimate the application value of a standard operating procedure (SOP) in the detection of syphilitic anticardiolipin reagin. Methods Clinical laboratories from 9 local hospitals in Shanghai participated the program. Quality control samples with unknown target value were qualitatively and quantitatively examined according to the uniform SOP in these laboratories with the same reagent and facility of horizontal reaction. External quality assessment (EQA) was carried out by using seven serum samples with no, or low (1∶ 128 dilution) to high (1∶1 dilution) concentrations of target before and after the implementation of SOP. The test results were statistically analyzed and the reasons for the detecting error were assessed. Results A total of 388 tests were performed in the 9 clinical laboratories. The total accuracy rate was 93.0%, including 40.2% in the detection of samples with 1 ∶ 8 dilution of target, 49.2% in the detection of samples with 1 ∶ 16 dilution of target, and 3.6% in the detection of samples with 1 ∶ 32 dilution of target. No forward bias was observed in these tests. There was a significant difference in the accuracy rate between the two times of EQA before and after the implementation of SOP (x2 = 4.17, P < 0.05). Conclusions The improved standard procedure for nontreponemal antigen test is beneficial to the decrease of testing error, and may provide a basis for the establishment of SOP and implementation of internal quality assessment.     

外周血单核细胞TLR2、TLR4与急性胰腺炎肺损伤的关系

急性胰腺炎（acute pancreatitis,AP）是胰酶引起的一种化学炎症反应性疾病,其出血坏死型（a-cute necrotizing pancreatitis,ANP）病情凶险,预后差,病死率高达50%左右。急性胰腺炎相关性肺损伤（acute pancreatitis-associated lung injury,APA-LI）是AP最常见的并发症,是患者早期死亡的重要原因.炎症介质的互相激活和级联反应是APALI的重要发生机制之一。     

慢性肾脏病患者α1酸性糖蛋白与微炎性反应及心血管疾病的关系

微炎性反应状态在慢性肾脏病(CKD)患者中普遍存在,是诱发心血管疾病(CVD)的主要因素[1].α1酸性糖蛋白(α1-AGP)和高敏C反应蛋白(hsCRP)是机体非特异性敏感的急性相蛋白,正常人血清中含量较低,但炎性反应患者体内α1-AGP和hsCRP水平急剧升高,这种特性使得α1-AGP和CRP成为目前最敏感的炎性反应标志物之一[2,3].     

介绍一种高灵敏的尿蛋白定性方法

目的寻找及建立一种简便、高灵敏的尿蛋白定性方法。方法用自制溴酚兰试剂与磺基水杨酸法测定尿蛋白并作比较。结果自制溴酚兰法的灵敏度远高于磺基水杨酸法和金标定量渗滤法,可达20mg/L。结论用自制溴酚兰试剂测定尿蛋白具有操作简便、灵敏、实用的优点,可用于微量白蛋白尿的筛选。     

全自动生化仪使用中项目间交叉污染的探讨

目的揭示全自动生化仪使用中项目间交叉污染的问题.方法以无机磷、镁和三酰甘油为对象,将内含磷酸盐、镁离子或甘油类组分的检测项目和这3个项目做几种组合,观察这些试剂对病人标本检测这3个项目的影响.结果说明这3个项目的检测设置对检测结果有明显的影响,差异达数倍.结论对这些分析项目的设置不能随意,必须注意位置和程序的设置.     

液体单试剂酶法测定血清碳酸氢根

目的建立一种稳定的单试剂酶法测定血清碳酸氢根（HCO3^-）。方法利用自配单试剂建立了两点法的检测方法，可满足全自动和手工测定，用Bayer 1650生化仪对本方法进行方法学评价。结果本法的线性范围可达50mmol／L，批内变异系数（CV）为2．48％，批间CV为3．24％，平均回收率102．8％，与血气分析仪（X）相关良好，Y=1．019X-0．745，r=0．9903，黄疸、脂血、溶血标本对结果几乎无影响，健康人静脉血HCO3^-浓度为21．5～32．3mmol／L。结论本法操作简便、反应特异、灵敏准确、试剂较稳定，适用临床检测。     

梅毒非特异性类脂质反应素抗体试验操作的改进及应用评价

Objective To estimate the application value of a standard operating procedure (SOP) in the detection of syphilitic anticardiolipin reagin. Methods Clinical laboratories from 9 local hospitals in Shanghai participated the program. Quality control samples with unknown target value were qualitatively and quantitatively examined according to the uniform SOP in these laboratories with the same reagent and facility of horizontal reaction. External quality assessment (EQA) was carried out by using seven serum samples with no, or low (1∶ 128 dilution) to high (1∶1 dilution) concentrations of target before and after the implementation of SOP. The test results were statistically analyzed and the reasons for the detecting error were assessed. Results A total of 388 tests were performed in the 9 clinical laboratories. The total accuracy rate was 93.0%, including 40.2% in the detection of samples with 1 ∶ 8 dilution of target, 49.2% in the detection of samples with 1 ∶ 16 dilution of target, and 3.6% in the detection of samples with 1 ∶ 32 dilution of target. No forward bias was observed in these tests. There was a significant difference in the accuracy rate between the two times of EQA before and after the implementation of SOP (x2 = 4.17, P < 0.05). Conclusions The improved standard procedure for nontreponemal antigen test is beneficial to the decrease of testing error, and may provide a basis for the establishment of SOP and implementation of internal quality assessment.     

曲美他嗪对不稳定性心绞痛患者血管内皮功能的保护作用

目的：观察曲美他嗪对不稳定性劳力型心绞痛患者血管内皮功能的保护作用,并探讨其可能原因。方法：61例不稳定性劳力型心绞痛患者,被随机分为常规治疗组（30例）和曲美他嗪组（31例）,常规治疗组给予扩冠、抗凝、调血脂等治疗,曲美他嗪组在常规治疗基础上加用曲美他嚷20rag,3次／d,服用8周。结果：血浆hs—CRP：治疗前曲美他嗪组和常规治疗组均较健康对照组升高;治疗后曲美他嗪组与常规治疗组均显著降低,曲美他嗪组较常规治疗组降低显著（P〈0．05）。治疗前FMD：曲美他嗪组和常规治疗组均较健康对照组明显降低;治疗后曲美他嗪组与常规治疗组均显著升高,曲美他嗪组较常规治疗组升高更加显著（P〈0．05）。结论：曲美他嗪在不稳定心绞痛患者治疗中能减轻血管炎症反应,改善血管内皮功能的作用。