米托蒽醌对中国仓鼠卵巢细胞周期的阻断

The effect of Mitoxantrone, a Potential anticancer chemotherapeutic agent, on the cell cycle progression of Chinese Hamster ovary (CHO) cells was investigated by microspectrophotometry. CHO cells incubated with the agent for either 30 min or 24 h were inhibited, to various degrees, from proliferation. The inhibition appeared in dose dependent fashion. A 10-fold dose of the drug was required for the 30 min group, as compared to the 24h group, to develop an equivalent inhibiting effect. When exponentially growing cells were treated with Mitoxantrone for 30 min, washed free of drug, and cultured in fresh medium for another 23.5 h a dose of 0.01 micrograms/ml had little or no effect on the distribution of CHO cells throughout the cell cycle at any point. However, a dose of 0.10-1.00 micrograms/ml was sufficient to cause a decrease of cells in G1 and an accumulation of cells in G2. Block cells had abnormally large nucleus.     

麦胚凝集素对红细胞血型糖蛋白A二级结构的影响

麦胚凝集素对红细胞血型糖蛋白Ａ二级结构的影响胡良高，姜厚理，段耀奎，苏雅娴（北京医科大学细胞生物学教研室１０００８３北京医科大学军事医学科学院）血型糖蛋白Ａ（ＧｌｙｃｏｐｈｏｒｉｎＡ，ＧＰＡ）是人红细胞膜上一种重要的单跨膜糖蛋白，是恶性疟原虫，麦胚凝...     

米托蒽醌对中国仓鼠卵巢细胞周期的阻断

作者用显微细胞光度计和细胞计数等方法研究米托蒽醌对中国仓鼠卵巢(CHO)细胞增殖周期的影响。结果表明,无论是30分钟还是24小时处理,米托蒽醌均抑制CHO细胞生长,且随药物浓度增大,抑制作用加强。用0.10μg/ml、1.00μg/ml米托蒽醌处理对数生长细胞30分钟,换新鲜培养液继续培养23.5小时后,与对照组相比,细胞核面积增大,核质疏松网状;显微细胞光度计测定未发现异常DNA增生;但群体中G_1期细胞频数减少,峰值后移,S期和G_2细胞频效显著增多,说明米托蒽醌使细胞停留在G_2期,阻断CHO细胞周期进程。