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目的 探讨卵巢上皮性癌组织中二肽基肽酶N(DPPIV)的表达及临床意义.方法 采用免疫组化法榆测378例卵巢上皮性癌组织中DPPIC蛋白的表达,采用原位杂交法检测86例卵巢上皮性癌组织中DPPIV mRNA的表达,42例正常卵巢组织作为对照.结果 DPPIV蛋白在卵巢上皮件癌组织中的总阳性表达率为92.9%(351/378),在正常卵巢组织中的总阳性表达率为59.5%(25/42),差异有统计学意义(P=0.001).DPPIV蛋门的表达与患者的发病年龄、肿瘤分化程度和FIGO分期无关(均P>0.05),而与肿瘤的组织学类型有关(P=0.005).DPPIC蛋白的表达水平越高,患者的生存期越短(P=0.023).DPPIV mRNA在卵巢上皮性癌组织巾的总阳性表达率为97.7%(84/86),在正常卵巢组织中的总阳件表达率为64.3%(27/42),差异有统计学意义(P<0.05).卵巢上皮性癌组织中DPPIV蛋白和mRNA的表达与定位一致,且二者旱止相关(P=0.001).结论 DPPIV在卵巢上皮性癌的发生发展中起着一定作用,其表达水平可能成为影响患者预后的因素. 相似文献
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目的:检测北欧和中国人乳癌患者TP、TS、DPD mRNA水平的差异。方法:应用定量RT-PCR法检测北欧乳癌患者中3种指标的表达,用半定量RT-PCR法检测中国乳癌患者中3种指标的表达。结果:仅中国标本TP mRNA水平显示肿瘤组织(0.44±0.23)高于正常组织(0.15±0.16),TP mRNA高表达率中国患者(56.67%)高于北欧患者(34.12%),60岁以下患者这种差异消失。TS、DPD mRNA高表达率在二地区无差异。结论:北欧和中国乳癌患者之间TP、TS、DPD mRNA表达无明显种族差异。 相似文献
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Objective To compare the short-term efficacy and adverse effects of docetaxe or oxaliplatin combined with capecitabine in the treatment of late-staged gastric cancer in aged patients. Methods Eighty-two aged patients with late-staged gastric cancer were randomly divided into two groups,of which 38 patients were treated group) ,and 44 patients were treated with oxaliplatin (100 mg/m2 ivgtt on 1st day) and eapecitabine (2000 mg/1 cycle). Results There is no failure of follow-up. In the docetaxe group,the effective rate was 52.63% (20/38) and 54.55 % (24/44) for the docetaxe and oxaliplatin group,respectively (P>0.05). The median progression-free survival(PFS) in the docetaxe group (6.1 months) was similar to that in the oxaliplatin group (6.3 months) (P>0.05). Gastrointestinal response,myelosuppression and neurotoxicity (Ⅰ or Ⅱ level) were the most common ad-verse effects observed in both groups (P>0.05). No chemotherapy-related death was observed. Conclusions The short-term efficacy of decetaxe or oxaliplatin combined with capecitabine in the treatment of late-staged gastric cancer in aged patients is similar,and the adverse effects are all within tolerance limits. 相似文献
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Objective To compare the short-term efficacy and adverse effects of docetaxe or oxaliplatin combined with capecitabine in the treatment of late-staged gastric cancer in aged patients. Methods Eighty-two aged patients with late-staged gastric cancer were randomly divided into two groups,of which 38 patients were treated group) ,and 44 patients were treated with oxaliplatin (100 mg/m2 ivgtt on 1st day) and eapecitabine (2000 mg/1 cycle). Results There is no failure of follow-up. In the docetaxe group,the effective rate was 52.63% (20/38) and 54.55 % (24/44) for the docetaxe and oxaliplatin group,respectively (P>0.05). The median progression-free survival(PFS) in the docetaxe group (6.1 months) was similar to that in the oxaliplatin group (6.3 months) (P>0.05). Gastrointestinal response,myelosuppression and neurotoxicity (Ⅰ or Ⅱ level) were the most common ad-verse effects observed in both groups (P>0.05). No chemotherapy-related death was observed. Conclusions The short-term efficacy of decetaxe or oxaliplatin combined with capecitabine in the treatment of late-staged gastric cancer in aged patients is similar,and the adverse effects are all within tolerance limits. 相似文献
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宫颈鳞癌组织中EphA2基因的表达 总被引:1,自引:1,他引:0
目的探讨宫颈鳞癌组织中EphA2的表达.方法应用免疫组化法观察EphA2在206例FIGO Ⅰ a-Ⅳb期的宫颈鳞癌组织中的表达,从中选择20例不同EphA2蛋白表达的病例,应用激光捕获显微切割技术(Laser cap-ture microdissection,LCM)从冰冻组织切片中选切大约50个宫颈鳞癌细胞,应用RT-PCR方法检测EphA2mRNA的表达.结果206例宫颈鳞癌组织中,EphA2蛋白阴性表达23例(11.2%),弱阳性表达94例(45.6%),阳性表达72例(35.0%),强阳性表达17例(8.3%);EphA2蛋白与其mRNA表达水平不完全一致;EphA2蛋白表达与宫颈鳞癌患者的年龄、FIGO分期及淋巴结转移无关,总生存期在EphA2染色强度0~3级之间差异有统计学意义(P<0.05).结论EphA2在宫颈鳞癌中的高表达预示患者的生存差.EphA2有望成为抗宫颈癌治疗的新靶点. 相似文献
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黑色素瘤分化相关基因-7(MDA-7)/白介素24(IL24),是IL-10家族的成员之一,主要表达于人体脾脏、胸腺、外周血白细胞等免疫组织器官和正常黑色素细胞。IL24是一种独特的抑制肿瘤细胞因子,通过自分泌旁分泌方式调控,抑制肿瘤生长、侵袭转移及血管生成,诱导凋亡,可以选择性的杀伤多种肿瘤细胞,产生放化疗增敏效应,而不伤害正常细胞。但是目前对于其作用的具体机制尚不明确。 相似文献
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靛玉红甲肟对HT-29细胞增殖和凋亡的影响及机制 总被引:1,自引:0,他引:1
背景与目的:近年来有报道称靛玉红甲肟(indirubin-3'-monoxime)在体内外实验中对部分实体肿瘤细胞具有明显的抑制作用,但尚未见其对人结肠癌HT-29细胞影响的研究报道,因而本文旨在研究其对HT-29细胞增殖和凋亡的影响及其机制.方法:MTT法检测不同浓度靛玉红甲肟处理HT-29细胞后细胞的增殖活性,制作生长抑制曲线.流式细胞仪检测凋亡率,RT-PCR检测细胞凋亡抑制基因survivin和bcl-2及凋亡促进基因Bax mRNA的变化.结果:靛玉红甲肟明显的抑制HT-29的增殖,其作用表现为剂量依赖性和时间依赖性(F=11.25,P<0.01).流式细胞仪检测发现:以10 μ mol/L的靛玉红甲肟处理HT-29细胞后,其凋亡率呈时间依赖性上升(F=195.25,P<0.01).RT-PCR检测发现HT-29细胞survivin(F=78.75,P<0.01)和Box(F=87.61,P<0.01)转录上升,而bcl-2转录显著下降(F=95.82,P<0.01).结论:靛玉红甲肟对人结肠癌HT-29细胞具有明显的增殖抑制和诱导凋亡作用,其作用机制与下调bcl-2/Bax比例有关. 相似文献
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34),两组差异有统计学意义,P=0 003.结论:结直肠癌患者血清sFas和组织FasL表达可能与结直肠癌术后肝转移呈正相关. 相似文献