CDC28蛋白激酶调节亚基2对卵巢癌A2780细胞丝足形成的影响及其机制研究

目的 探讨CDC28蛋白激酶调节亚基2(CKS2)对A2780细胞丝足形成的影响及其可能机制.方法 采用慢病毒介导的shRNA敲除A2780细胞的CKS2基因,显微镜下观察细胞丝足的变化;采用细胞划痕实验检测A2780细胞迁移能力的变化;采用real time PCR检测CKS2敲除对CDC42两种剪接变异体(CDC42-V1和CDC42-V2)表达的影响,以及不同卵巢癌标本中CKS2、CDC42-V1和CDC42-V2剪接变异体的表达情况.结果 CKS2表达抑制后,A2780细胞丝足明显减少,细胞迁移能力明显减弱(P＜0.05),CDC42-V1mRNA表达降低,而CDC42-V2 mRNA表达升高(P＜0.05).Real time PCR结果显示,卵巢癌组织标本中CKS2及CDC42-V1的表达高于对应的正常卵巢组织,而CDC42-V2的表达低于对应的正常卵巢组织(p＜0.05).结论 CKS2通过调控CDC42的选择性剪接而影响细胞丝足的形成,进而影响卵巢癌细胞A2780的迁移能力.     

铜藻岩藻多糖匀浆提取工艺及抗氧化活性分析

目的 优化铜藻岩藻多糖的匀浆提取工艺,并测定其抗氧化活性。方法 以岩藻多糖的提取率为指标,基于单因素试验的基础上,采用响应面法对铜藻中岩藻多糖的提取工艺进行优化,并对其抗氧化活性进行验证。结果 最佳提取工艺条件为提取时间6 min、提取温度94℃、液料比34 mL·g^-1和提取次数2次。在此条件下,多糖的提取率为14.76%。抗氧化活性研究结果表明,铜藻岩藻多糖在质量浓度为0.2～10.0 mg·mL^-1范围内有一定的抗氧化活性,对DPPH自由基、ABTS⁺自由基清除率的IC₅₀分别为0.22、0.41 mg·mL^-1。结论 该提取工艺高效,可为铜藻岩藻多糖的制备及应用提供理论依据。     

健康样板房

怎样才能装修一套无毒、无污染的健康住房呢?这是每个人,尤其是每个家庭的女主人十分关心的问题。为此,我们邀请了几位室内装饰协会的专家,特地为大家精心打造了一套“健康样板房”,供你参考。     

胰岛素抵抗小鼠小肠类器官模型构建及黄诺玛苷对此模型肠黏膜屏障的保护作用

目的 构建胰岛素抵抗(IR)小鼠小肠类器官模型,研究两色金鸡菊黄酮类成分黄诺玛苷对肠黏膜屏障的保护作用。方法 (1)构建C57BL/6J和db/db小鼠小肠类器官模型,3D免疫荧光染色观察小肠类器官细胞核核抗原(Ki-67)、上皮细胞E钙黏蛋白(E-cad)、溶菌酶(Lyz)和黏蛋白2(MUC-2)表达,实时荧光定量PCR(RT-q PCR)检测纤连蛋白(Fn)、胰高血糖素样肽1(GLP-1)和肽YY(PYY)m RNA表达;Western印迹法检测Fn,GLP-1和PYY蛋白表达;ELISA检测Lyz分泌水平。(2)将构建的小鼠小肠类器官分为5组：以C57BL/6J小鼠小肠类器官为正常对照组,db/db小鼠小肠类器官为IR模型组,db/db小鼠小肠类器官用黄诺玛苷25,50和100μmol·L^-1处理48 h为IR模型+黄诺玛苷组,RT-q PCR检测各组类器官Lyz m RNA表达,Western印迹法检测Lyz蛋白表达。结果 (1) C57BL/6J和db/db小鼠小肠类器官培养第6天形成管腔结构清晰的环状结构,IR小鼠小肠类器官模型建立成功。3D免疫荧光检...     

转录组测序分析昆仑雪菊醇提物黄诺玛苷对胰岛素抵抗小鼠小肠类器官的影响

目的：探讨黄诺玛苷对胰岛素抵抗小鼠小肠类器官转录组的影响。方法：首先建立C57BL/6J和db/db小鼠小肠类器官；采用免疫荧光法检测细胞核核抗原（ki-67）和上皮细胞E-钙黏蛋白（E-cadherin）表达；将小肠类器官分为3组，分别是以C57BL/6J小鼠小肠类器官为正常组、db/db小鼠小肠类器官为模型组和db/db小鼠小肠类器官黄诺玛苷干预组为给药组（FM组）；采用蛋白免疫印迹法（Western blot）检测3组小肠类器官胰高血糖素样肽-1(GLP-1)蛋白的表达；并对3组样本进行转录组测序。结果：小肠类器官培养第6天形成围绕管腔周围的环状结构，初步建立了小肠类器官培养模式；免疫荧光检测结果显示小肠类器官均表达ki-67和E-cadherin;Western blot结果显示黄诺玛苷可增加GLP-1蛋白的表达；差异基因表达分析中，模型组与正常组相比差异表达基因共有1 862个，FM组与模型组相比差异表达基因共有2 282个；通过蛋白质-蛋白质相互作用（PPI）网络分析2组共同表达的差异基因，得到Nr1i3、Cyp2c44、Ugt2b1、Gsta1、Gstm2、Ptgs1、G...     

Analysis of clinical features in children with McCune-Albright syndrome北大核心CSCD

Objective To summarize and analyze the clinical features of McCune-Albright syndrome (MAS) in 26 children, to improve the understanding of MAS diagnosis and treatment, and to achieve early clinical diagnosis of MAS. Methods The clinical data of 26 children with MAS treated in Department of Pediatrics, Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology from August 2011 to June 2021 were retrospectively analyzed.Their clinical characteristics were summarized and studied.t-test, Mann-Whitney U test, χ 2 test or Fisher′s exact probability method was used for comparison between groups. Results (1) Among the 26 MAS patients enrolled, there were 22 females and 4 males.The average onset age of female and male patients was (5.87±2.94) years old and (7.48±3.36) years old, respectively.(2) In female patients, there were 7 cases with the typical triad and 15 cases with the atypical triad.(3) Female patients had the first symptom of vaginal bleeding (8/22) and breast development (14/22). Among the 4 male children, 1 case had " fracture" and 3 cases " lagged behind their peers in height" at the first visit.(4) Compared with the breast development group, the vaginal bleeding group had an earlier onset age[(4.06±1.88) years old vs.(7.82±1.82) years old] (t=5.023, P<0.001), earlier bone maturation[(1.26±0.07) vs.(1.09±0.13)] (t=2.933, P<0.05), a greatly lowered predicted adult height[(-2.16±0.98) SDS vs.(-0.96±1.09) SDS](t=1.352, P<0.05), a lower blood phosphorus level[(1.41±0.14) nmol/L vs.(1.67±0.24) nmol/L] (t=1.941, P<0.05), and a significantly elevated alkaline phosphatase level[339(313, 656) U/L vs.243(205, 452) U/L] (U=1.000, P<0.05). All patients (8 cases) in the vaginal bleeding group had fibrous dysplasia of bone.(5) Ten patients progressed to central precocious puberty (CPP). They showed an older average age of onset[(7.27±2.69)years old vs.(4.69±2.68)years old] (U=44.000, P<0.05), significantly earlier bone maturation at diagnosis[(1.23±0.11) vs.(1.01±0.13)] (t=1.834, P<0.05), and a lower predicted adult height[(152.00±4.62) cm vs.(162.10±6.91) cm] (t=3.805, P<0.05), compared with those who did not progress to CPP.(6) Eleven children developed polyostotic fibrous dysplasia of bone, and most common type (8 cases) was polyostotic fibrous dysplasia of bone, primarily at lower limb bones and skulls.(7) Of the 26 children, 20 cases had scattered Café au lait pigments on the skin.The Café au lait pigmented skin lesions in 35% (7/20) cases crossed the midline. Conclusions With complicated clinical manifestations, MAS is more common and occurs earlier in girls than boys.It is difficult to clinically diagnose MAS in boys due to the insidious onset and atypical symptoms.Female MAS patients with vaginal bleeding as the first symptom usually have an early age of onset, advanced bone age and lower predicted adult height, so they should be monitored and evaluated clinically.Vaginal bleeding is significantly associated with polyostotic fiber dysplasia of bone in MAS patients.Therefore, it is recommended that patients with vaginal bleeding should undergo a routine bone single-photon emission computed tomography scan.This helps understand the situation of occult fiber dysplasia of bone.Patients with later diagnosis and advanced bone age should be aware of the possibility of progression to CPP. © 2022 Chinese Journal of Applied Clinical Pediatrics. All rights reserved.     

岩藻多糖对肠道菌群与慢性肾衰竭作用研究进展

岩藻多糖具有抗氧化、抗炎、抗凝血等功效，已成为研究热点。慢性肾功能衰竭（CRF）近年来发病率逐年上升。肠-肾轴研究发现肠道菌群（GM）与CRF之间存在双向调节。GM能够通过平衡内稳态调控机体健康，已成为治疗CRF潜在新靶点。岩藻多糖被作为一种微生物调节剂能干预GM失调进而控制CRF进程，预防加速肾衰竭潜在的危险因素。因此，本文探讨岩藻多糖对GM和CRF作用及GM与CRF双向调节机制，为后续岩藻多糖的开发和利用提供研究方向与思路。