叔丁基氢过氧化物对人胎儿主动脉平滑肌细胞生长的影响

利用体外培养的人胎儿主动脉平滑肌细胞作为实验材料,观察了不同浓度脂氢过氧化物——叔丁基氢过氧化物(tbOOH)对不同代数的平滑肌细胞生长的影响。10~(-4)mol/L tbOOH对第9代细胞有促分裂作用,10~(-3)及10~(-4)mol/L两种浓度的tbOOH对第17代细胞均显示出生长抑制作用。tbOOH对平滑肌细胞的生长抑制作用与其对细胞的损伤有关。     

用人LDL培养的胎儿主动脉平滑肌细胞内SOD活性的测定

本文介绍了用人LDL培养的胎儿主动脉平滑肌细胞内SOD活性变化的趋势,结果显示,LDL在促进细胞增生的同时,能诱导细胞内SOD活性增高;而当细胞生长到一定程度后,SOD活性下降,出现细胞死亡;这些变化与动脉粥样硬化的病变相一致。     

氧化修饰的低密度脂蛋白对培养的人脐静脉内皮细胞的影响

以培养的人脐静脉内皮细胞(EC)为损伤模型,观察到氧化修饰的低密度脂蛋白(O—LDL,100μg/ml和叔丁基脂氢过氧化物(tbooH,10~(-2)mM)对内皮细胞的硒—谷胱甘肽过氧化物酶(SeGSH—Px)的活性,前列腺环素(PGI_2)的生成,脂质过氧化物(Lpo)含量及SeGSH—Px/Lpo比值有明显的时间依赖效应,即随作用时间的延长,O—LDL和tbooH使EC的SeGSH—Px活性下降,PGI_2生成减少,SeGSH—Px/Lpo比值降低;而Lpo含量则增加。提示O—LDL对EC的损伤实质是脂质过氧化物的作用。     

电泳纯的LDL的快速提取及Cu~(++)对其氧化修饰的影响

本文报道一种快速提取低密度脂蛋白(LDL)及其氧化修饰的方法,获得的LDL可达电泳纯。用CuSO_4催化LDL氧化修饰,其脂质过氧化物(LPO)含量为作用前及用EDTA保护(200mmol/L)的LDL的5倍(P<0.01)。聚丙烯酰胺凝胶电泳可将修饰的LDL与自然LDL予以区别。     

Changes of peroxide dismutase activity and malondialdehyde level in the smooth muscle cells of human fetal aorta cultured in vitro by low density lipoprotein conditional medium

Here are reported the changes of superoxide dismutase(SOD)activity andmalondialdehyde(MDA)in the smooth muscle cells of human fetal aorta cultured in vitro with lowdensity lipoprotein(LDL)conditional medium.The results showed that a single concentration of hu-man LDL(50μg/ml)stimulated proliferation of smooth muscle cells,and the SOD activityof the cells in the experimental group was higher,from the first to the fifth cultured day whenthe cells had a active proliferation,than that of the control cells.This suggests that LDL might in-duce the increase of SOD activity.At the seventh day,as the cells were in inactive proliferation,SOD activity was low and the difference was significant as compared with that at the fifth day ofthe same group.This also indicates that the SOD activity may be related to the cell proliferation.MDA level within the cells of the esperimental group was lowered with the cell active proliferationand the increase of SOD activity,but when the cells were in inactive proliferation and the SOD ac-tivity decreased,it will remained low.