Effect of photobiomodulation on CCC-ESF reactive oxygen species steady-state in high glucose mediums

Lasers in Medical Science - Delayed wound healing is one of the most challenging complications of diabetes mellitus (DM) in clinical medicine, and it is related to the excessive generation of...     

间充质干细胞对小鼠活化B细胞微小核糖核酸155和肿瘤坏死因子-α表达的影响

目的 研究间充质干细胞(MSCs)对小鼠活化B细胞微小核糖核酸155(miRNA-155)和肿瘤坏死因子-α(TNF-α)表达的影响,探讨MSCs对B细胞的调控机制.方法 以小鼠脾脏原代B细胞为研究模型,在B细胞受体(BCR)刺激下,与人脐带来源的MSCs共培养3d后,收集悬浮的B细胞,进行细胞计数,实时定量PCR检测B细胞miRNA-155和TNF-α mRNA水平,酶联免疫吸附试验(ELISA)法检测共培养上清中细胞因子免疫球蛋白G(IgG)、TNF-α、转化生长因子β(TGF-β)、前列腺素E2(PGE2)、白细胞介素(IL)-10和吲哚胺2,3-双加氧酶(IDO)水平.结果 B细胞活化后增殖明显,MSCs能轻微抑制B细胞增殖(F =5.39,P＜0.05).B细胞活化后miRNA-155和TNF-α mRNA均上调明显,MSCs能部分逆转miRNA-155和TNF-α mRNA的升高(F=18.85,P＜0.01和F=10.26,P＜0.05).B细胞活化后共培养上清TNF-α、IgG升高明显,MSCs能部分缓解TNF-α和IgG的升高(F=27.94,P＜0.01和F=4.81,P＜0.05).在MSCs和B细胞共培养上清中,PGE2升高尤为明显,其次是IDO.结论 MSC可能通过可溶性因子PGE2和IDO抑制活化B细胞miRNA-155和TNF-α的表达,MSCs可能通过抑制B细胞治疗免疫疾病发挥重要作用.     

An ultra-robust fingerprinting method for quality assessment of traditional Chinese medicine using multiple reaction monitoring mass spectrometry

Chromatographic fingerprinting has been perceived as an essential tool for assessing quality and chemical equivalence of traditional Chinese medicine. However, this pattern-oriented approach still has some weak points in terms of chemical coverage and robustness. In this work, we proposed a multiple reaction monitoring (MRM)-based fingerprinting method in which approximately 100 constituents were simultaneously detected for quality assessment. The derivative MRM approach was employed to rapidly design MRM transitions independent of chemical standards, based on which the large-scale fingerprinting method was efficiently established. This approach was exemplified on QiShenYiQi Pill (QSYQ), a traditional Chinese medicine-derived drug product, and its robustness was systematically evaluated by four indices: clustering analysis by principal component analysis, similarity analysis by the congruence coefficient, the number of separated peaks, and the peak area proportion of separated peaks. Compared with conventional ultraviolet-based fingerprints, the MRM fingerprints provided not only better discriminatory capacity for the tested normal/abnormal QSYQ samples, but also higher robustness under different chromatographic conditions (i.e., flow rate, apparent pH, column temperature, and column). The result also showed for such large-scale fingerprints including a large number of peaks, the angle cosine measure after min-max normalization was more suitable for setting a decision criterion than the unnormalized algorithm. This proof-of-concept application gives evidence that combining MRM technique with proper similarity analysis metrices can provide a highly sensitive, robust and comprehensive analytical approach for quality assessment of traditional Chinese medicine.     

PBL教学法在《经络腧穴学》教学实践中的应用

《经络腧穴学》是针灸基础医学和临床医学之间的桥梁性课程。借鉴问题式学习(PBL)教学模式,结合临床设计问题,以学生为主、教师引导的方式实施教学,为激发学生主动学习的积极性,笔者从贴近临床设计典型问题、学习小组课堂发言、重视描画经脉循行和腧穴定位实践训练、总结分析并做出综合评价等方面实施课堂教学,以提高学生的综合创新能力,从而提高教学质量。     

两种试剂对泌尿生殖道解脲支原体和人型支原体的检测及临床应用

[目的]观察国产及进口试剂对泌尿生殖道解脲支原体(UU)及人型支原体(MH)的培养及药敏结果,判断两种试剂对UU及MH检出的阳性率及药敏结果在临床上的应用。[方法]非淋菌性尿道炎(NGU)泌尿生殖道标本同时接种国产与进口试剂,进行UU及MH培养、药敏试验,并按要求操作并观察记录结果。[结果]试剂间阳性率UUx2=0.00(P>0.05),MHx2=1.50(P>0.05)。UU敏感药物国产试剂强力霉素(多西环素)89.39%、美满霉素(米诺环素)87.90%、四环素84.80%、交沙霉素81.80%;进口试剂的敏感药物是:交沙霉素98.50%、原始霉素(普那霉素)98.50%、多西环素95.45%、四环素89.40%。[结论]两种试剂培养结果无明显差异,药敏结果对临床医师应用抗生素治疗意义较为重要,从卫生经济学角度考虑,宜选择国产试剂作为常规检测试剂,如果医院使用进口抗生素较多,则需选择符合程度较高的试剂盒。应用多西环素、交沙霉素治疗UU及MH感染疗效较好。     

轻度认知障碍患者EEG动力学特征提取与分类方法研究进展

脑电图可用于对轻度认知障碍的病理性变化进行评估。近年来,脑电领域的特征提取和分类方法广泛地应用到对轻度认知障碍疾病的诊断中。首先从局部耦合与全局同步两个方面,深入分析轻度认知障碍患者脑电信号特征提取方法的应用情况及其优势和不足,而后对当前轻度认知障碍患者脑电信号特征进行分类的多种方法进行详细总结与分析,如支持向量机、k均值以及近年来应用广泛的卷积神经网络等,最后对该领域脑电动力学特征提取与分类方法的未来发展趋势进行展望。     

青蒿素哌喹片红外光谱鉴别方法的探讨

目的：对青蒿素哌喹片进行鉴别。方法：采用不同溶剂提取青蒿素哌喹片中的青蒿素和哌喹,分别测定各自红外谱图。结果：除了青蒿素红外光谱图在1 574 cm-1 处出现一组哌喹干扰峰外,青蒿素哌喹片经提取后的红外谱图均与《药品红外光谱集》中青蒿素和哌喹标准谱图一致。结论：该方法稳定可靠、专属性强,可用于青蒿素哌喹片的鉴别。     

去壁灵芝孢子粉片的含量测定与免疫调节及心脏保护功能研究

目的 研究并评价“仙芝3号”去壁灵芝孢子粉片的免疫调节和心脏保护作用。方法 首先运用UPLC-Q-TOFMS和比色法分析“仙芝3号”去壁灵芝孢子粉片的主要化学成分,考察其对斑马鱼巨噬细胞减少症模型、斑马鱼心衰模型、H2O2诱导的心肌及内皮细胞氧化应激模型、脂多糖诱导的小胶质细胞炎症模型的影响,通过巨噬细胞形成能力、巨噬细胞吞噬能力、抗神经炎症能力、心脏收缩舒张改善能力、抗心肌及内皮细胞氧化应激损伤能力等多个指标评估其免疫调节及心脏保护作用。结果 “仙芝3号”去壁灵芝孢子粉片可以提高巨噬细胞形成及吞噬能力,改善心脏收缩舒张功能,减少神经炎症并缓解心肌及内皮细胞因氧化应激而造成的损伤,从而起到免疫调节和心脏保护作用。结论 “仙芝3号”去壁灵芝孢子粉片有望用于免疫功能调节及心脏功能保护。