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排序方式: 共有154条查询结果,搜索用时 31 毫秒
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Immunogenicity of fowlpox virus expressing the avian influenza virus H5 gene (TROVAC AIV-H5) in cats 总被引:4,自引:0,他引:4
Karaca K Swayne DE Grosenbaugh D Bublot M Robles A Spackman E Nordgren R 《Clinical and diagnostic laboratory immunology》2005,12(11):1340-1342
Vaccination of cats with fowlpox virus expressing the avian influenza (AI) virus H5 hemagglutinin gene (TROVAC AI) resulted in detectable hemagglutination inhibition (HI) antibody responses to the homologous A/Turkey/Ireland/1378/83 (H5N8) (A/tky/Ire/83) AI virus antigen. The HI antibody responses to heterologous A/Chicken/Indonesia/7/03 (H5N1) (A/ck/Indonesia/03) AI virus antigen were also detected in all vaccinated cats, but only after booster vaccinations. The vaccine described in this study and other poxvirus-vectored vaccines may be of value for the prophylaxis of AI virus-associated morbidity and mortality in mammals. 相似文献
3.
The movement of highly pathogenic avian influenza (H5N8) virus across Eurasia and into North America and the virus’ propensity to reassort with co-circulating low pathogenicity viruses raise concerns among poultry producers, wildlife biologists, aviculturists, and public health personnel worldwide. Surveillance, modeling, and experimental research will provide the knowledge required for intelligent policy and management decisions. 相似文献
4.
Callison SA Riblet SM Oldoni I Sun S Zavala G Williams S Resurreccion RS Spackman E García M 《Journal of virological methods》2007,139(1):31-38
In this study, the development and validation of a real-time (ReTi) PCR assay is described using a Taqman labeled probe for the detection and quantitation of infectious larygotracheitis virus (ILTV) in chickens. The ReTi ILTV assay was highly specific with a quantitation limit of 100 viral template copies per amplification reaction. In experimentally infected, birds during early acute stages of infection, an average of 6.67 log(10) viral template copies/amplification reaction were detected, while at chronic late stages of infection an average of 2.86-3.27 log(10) viral template copies/amplification reaction were detected. A total of 246 tracheal swab samples collected from natural outbreaks of the disease were tested by virus isolation and the ReTi ILTV assay. Both assays agreed in 37% of the samples tested and the ReTi ILTV assay detected approximately 3.7 times more positives samples than virus isolation. A minimum of 5 log(10) viral template copies/amplification reaction were required from a tracheal swab to render a virus isolation positive result. In conclusion, the ReTi ILTV assay was highly specific, sensitive, reproducible, and capable of reliably quantifying viral nucleic acid directly from clinical samples. 相似文献
5.
Spackman LA Boyd SG Towell A 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》2007,177(1):21-30
Somatosensory processing of duration and frequency changes was investigated using event-related potentials to vibrotactile
stimuli. Intermittent vibration to the fingertips of either hand was presented using a two-stimulus odd-ball paradigm (deviant
P = 0.10). One group (N = 12, 18–38 years) was presented with stimulus pairs of 20/70, 50/150 and 170/250 ms. A second group (N = 10, 19–34 years) was tested using frequency pairs of 200/70 Hz. A psychophysical study examined the subjects’ ability to
discriminate between different stimulus pairs. A clear negative shift in the response to the deviant stimulus was recorded
with all the stimulus conditions used in both experiments. Both frequency changes and duration increments/decrements revealed
an initial negativity in the subtraction waveform with a mean onset of 90–170 ms and a following positivity, both of which
were dependent on the duration of the stimulus used. A significant decrease in the amplitude of both components was observed
with the 170/250 ms pairing, coinciding with a positive correlation between individual discrimination performance and amplitude.
These results support the existence of a somatosensory mismatch response with features similar to those of the aMMN and highlight
the relevance of the somatosensory-specific positivity. Results from the duration experiment also resolve some of the discrepancies
between previous studies. 相似文献
6.
V Riley W Braun M Ishizuka D Spackman 《Proceedings of the National Academy of Sciences of the United States of America》1976,73(5):1707-1711
Spleen antibody-forming cells of mice yield a 3- to 10-fold increase in their response to sheep erythrocyte antigen if they are acutely infected by lactate dehydrogenase-elevating virus. This early stimulation is replaced by a long-term inhibition of the antibody-forming cells as the viremia goes into its persisting chronic stage. These contrasting immunological phenomena are examined as contributing factors responsible for the enhancement by this virus of asparaginase (EC 3.5.1.1; L-asparagine amidolydrolase) therapy against leukemia in mice, and for the alteration of the susceptibility of mice to various neoplastic processes. 相似文献
7.
Eldon Spackman Mark Sculpher Jo Howard Moira Malfroy Charlotte Llewelyn Louise Choo Renate Hodge Tony Johnson David C. Rees Karin Fijnvandraat Melanie Kirby‐Allen Sally Davies Lorna Williamson 《European journal of haematology》2014,92(3):249-255
The study's objective was to assess the cost‐effectiveness of preoperative transfusion compared with no preoperative transfusion in patients with sickle cell disease undergoing low‐ or medium‐risk surgery. Seventy patients with sickle cell disease (HbSS/Sß0thal genotypes) undergoing elective surgery participated in a multicentre randomised trial, Transfusion Alternatives Preoperatively in Sickle Cell Disease (TAPS). Here, a cost‐effectiveness analysis based on evidence from that trial is presented. A decision‐analytic model is used to incorporate long‐term consequences of transfusions and acute chest syndrome. Costs and health benefits, expressed as quality‐adjusted life years (QALYs), are reported from the ‘within‐trial’ analysis and for the decision‐analytic model. The probability of cost‐effectiveness for each form of management is calculated taking into account the small sample size and other sources of uncertainty. In the range of scenarios considered in the analysis, preoperative transfusion was more effective, with the mean improvement in QALYs ranging from 0.018 to 0.206 per patient, and also less costly in all but one scenario, with the mean cost difference ranging from ?£813 to £26. All scenarios suggested preoperative transfusion had a probability of cost‐effectiveness >0.79 at a cost‐effectiveness threshold of £20 000 per QALY. 相似文献
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Analysis of the high-frequency (HF) components of the QRS complex has been shown to be a more sensitive indicator of myocardial ischemia and infarction than conventional ST segment analysis in settings outside of the operating room. In this study, we documented the effect of general anesthesia on HF QRS analysis in healthy patients as the first step in determining the potential of this technique for monitoring anesthetized patients. HF QRS electrocardiograms (ECGs) were obtained from all 12 ECG leads in 30 healthy subjects before and after the induction of anesthesia. When compared with preinduction values, there were significant postinduction changes in multiple variables of the HF QRS in many leads studied that were within previously described normal limits. Additional study is needed to understand the potential of this monitoring technique for enhancing detection of myocardial ischemia in the anesthetized population. 相似文献