华坪县2013-2015年健康人群麻疹抗体水平监测结果分析

目的了解华坪县健康人群麻疹抗体水平,发现工作薄弱地区和免疫空白人群,为实现消除麻疹的目标提供科学依据。方法采用分层抽样方法对华坪县8个乡镇不同年龄段健康人群开展麻疹抗体监测,采用间接酶联免疫吸附试验(ELISA间接法)检测血清麻疹Ig G抗体。结果 2013-2015年共检测1 106人,麻疹抗体总阳性率96.47%。其中,阳性率较低的是2014年(93.13%),人群是8~17月龄组(89.22%)。尚有新庄乡(89.92%)、船房乡(93.98%),通达乡(94.86%)3个乡镇麻疹抗体阳性低于95%。有免疫史者人群阳性率较无免疫史者高(χ~2=5.1830,P=0.023),男女性别间无差异。结论华坪县人群麻疹免疫效果较好,但存在免疫薄弱地区和免疫空白人群,应结合该监测结果,加强重点乡镇麻疹疫苗两剂次接种工作和抗体阴性人群的重新免疫接种。     

Utility of Tissue Similarity Maps Based on Relative Cerebral Blood Volume for Grading Gliomas as Validated by Histological Results

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N-三甲基壳聚糖对促进阿昔洛韦滴眼液眼部滞留性的体内外考察

目的对不同季铵化程度(DQ)的N-三甲基壳聚糖(TMC)促进阿昔洛韦(ACV)滴眼液眼部滞留性进行考察。方法将浓度为0.5%,DQ分别为20%、40%及60%的TMC(TMC20,TMC40,TMC60)加入ACV滴眼液中;以同浓度壳聚糖(CS)ACV滴眼液为阳性对照,以普通ACV滴眼液为阴性对照,选用家兔为实验动物,采用悬挂泡技术和束缚泡技术进行离体眼球表面接触角及解吸附动力学进行研究,同时在各种ACV滴眼液中分别加入0.05%的荧光素钠为示踪剂,在体研究各种ACV滴眼液在家兔眼部的滞留性。结果与普通ACV滴眼液相比,含CS及TMC的滴眼液在角膜表面的接触角明显降低,解吸时间及在体滞留时间明显延长(P<0.05);与同浓度的CS ACV阳性对照相比,TMC20促进滞留作用较弱,而TMC40作用差异无显著性(P>0.05),而TMC60的作用明显较强(P<0.05)结论 TMC可显著增加ACV的眼部滞留性,且与其DQ呈正相关。     

Mixed Reality Combined with Three‐Dimensional Printing Technology in Total Hip Arthroplasty: An Updated Review with a Preliminary Case Presentation

Three‐dimensional (3D) printing technology, virtual reality, and augmented reality technology have been used to help surgeons to complete complex total hip arthroplasty, while their respective shortcomings limit their further application. With the development of technology, mixed reality (MR) technology has been applied to improve the success rate of complicated hip arthroplasty because of its unique advantages. We presented a case of a 59‐year‐old man with an intertrochanteric fracture in the left femur, who had received a prior left hip fusion. After admission to our hospital, a left total hip arthroplasty was performed on the patient using a combination of MR technology and 3D printing technology. Before surgery, 3D reconstruction of a certain bony landmark exposed in the surgical area was first performed. Then a veneer part was designed according to the bony landmark and connected to a reference registration landmark outside the body through a connecting rod. After that, the series of parts were made into a holistic reference registration instrument using 3D printing technology, and the patient's data for bone and surrounding tissue, along with digital 3D information of the reference registration instrument, were imported into the head‐mounted display (HMD). During the operation, the disinfected reference registration instrument was installed on the selected bony landmark, and then the automatic real‐time registration was realized by HMD through recognizing the registration landmark on the reference registration instrument, whereby the patient's virtual bone and other anatomical structures were quickly and accurately superimposed on the real body of the patient. To the best of our knowledge, this is the first report to use MR combined with 3D printing technology in total hip arthroplasty.     

中日友好医院分型股骨头坏死腓骨植入治疗的三维有限元分析

文题释义：股骨头坏死中日友好医院分型的有限元分析：根据李子荣等提出的中日友好医院分型，建立股骨头坏死三维模型，分为 M型(内侧型)、C型(中央型)和 L型(外侧型)，其中 L型包括L1型(次外侧型)、L2型(极外侧型)和 L3型(全头型)。通过对建立的模型进行有限元分析，为该分型的保髋治疗提供了一定力学依据，显示外侧柱的存留是精准预防塌陷的重要因素，为进一步实现个体化治疗提供力学基础。 腓骨支撑坏死股骨头保髋手术：是对于早中期股骨头坏死需要保留股骨头患者进行的一种手术方式。首先需对股骨头进行髓芯减压，清除一定坏死骨，空腔填塞松质骨(髂骨为主)，打压结实后植入腓骨(异体或自体)支撑，给坏死区的提供力学支撑及生物学修复，预防股骨头进一步坏死及塌陷。 背景：研究报道股骨头坏死的保髋疗效与外侧柱存留密切相关，中日友好医院分型是根据三柱结构确立的，对股骨头塌陷的预测准确性高。 目的：建立股骨头坏死中日友好医院分型各分型仿真的三维有限元模型，通过有限元分析各分型腓骨植入的力学变化，探讨外侧柱存留对保髋疗效的意义，为该分型的塌陷精准预测提供基础。 方法：建立正常股骨头、中日友好医院分型(M型、C型、L1型、L2型、L3型)股骨头坏死及其腓骨植入3组11种三维有限元模型，运用ANSYS软件进行有限元分析计算，观察各组模型的最大应力值、最大位移值及股骨头内部载荷传递模式。 结果与结论：①坏死组位移最大，应变最大，且因坏死分型不同而位移不同，位移变化如下：M型相似文献   

中医药治疗糖尿病周围神经病变的研究进展

糖尿病渐以年轻化的趋势呈现,糖尿病周围神经病变(DPN)是糖尿病最常见的慢性并发症之一,因其高致残率严重影响病患的生活品质。本文简单阐述糖尿病周围神经病变的病因,并就中医药的角度对该病的治疗概况进行综述,以期为临床诊治提供新见解和新研究策略。     

电针对缺血性脑卒中大鼠记忆功能及脑内突触囊泡蛋白的影响

观察电针对缺血性脑卒中大鼠记忆功能及脑内突触囊泡蛋白（synaptic vesicular protein，SYN）的影响，探讨其可能的作用机制。方法 选取90 只SD大鼠随机分为模型组、电针组、假手术组，每组各30只。线拴法制作急性大脑中动脉缺血大鼠模型。电针组取“百会”“水沟”“内关”“三阴交”穴位，应用“醒脑开窍”法进行电针，每天电针30 min，连续电针6 d休1 d，7 d为一个疗程，首次电针干预在造模成功24 h后进行。模型组和假手术组不进行电针干预。大鼠分别按7、14、21 d 3个亚组进行运动及记忆功能评分，TTC染色测定脑梗死体积、Western blot检测脑内SYN的蛋白表达。结果 电针组运动功能评分较模型组显著降低（P<0.01），电针组进入隐藏区潜伏期时间较模型组显著缩短（P<0.01），电针组脑梗死率较模型组显著缩小（P<0.01），SYN的蛋白表达电针组较模型组明显增强（P<0.01）。假手术组无神经功能缺损及脑梗死灶，SYN表达最弱。结论 电针干预能减小脑梗死体积，上调脑内SYN的表达，进而促进缺血性脑卒中大鼠的记忆及运动等神经功能的恢复。     

妊娠期高血压疾病早期肾损害的监测进展

妊娠妇女随着孕周的增加,其本身及胎儿代谢产物逐渐增多,加之循环血容量增加,使肾脏总体负荷加重,容易并发肾损害。妊娠期高血压疾病是妊娠期特有的疾病,其病理生理变化为全身小血管痉挛、内皮损伤及局部缺血;肾脏最先受累。严重的肾损害会导致较高的母儿患病率和病死率,而肾脏损害存在无症状阶段,通过筛查才能发现相对早期的肾损害。探索妊娠期高血压疾病早期肾损害敏感性指标,可早发现、早干预,从而减少不良产科结局。     

Characterization of membrane occupation and recognition nexus repeat containing 3, meiosis expressed gene 1 binding partner,in mouse male germ cells

Mammalian spermatogenesis is a well-organized process of cell development and differentiation. Meiosis expressed gene 1 (MEIG1) plays an essential role in the regulation of spermiogenesis. To explore potential mechanisms of MEIG1''s action, a yeast two-hybrid screen was conducted, and several potential binding partners were identified; one of them was membrane occupation and recognition nexus repeat containing 3 (MORN3). MORN3 mRNA is only abundant in mouse testis. In the testis, Morn3 mRNA is highly expressed in the spermiogenesis stage. Specific anti-MORN3 polyclonal antibody was generated against N-terminus of the full-length MORN3 protein, and MORN3 expression and localization was examined in vitro and in vivo. In transfected Chinese hamster ovary cells, the antibody specifically crossed-reacted the full-length MORN3 protein, and immunofluorescence staining revealed that MORN3 was localized throughout the cytoplasm. Among multiple mouse tissues, about 25 kDa protein, was identified only in the testis. The protein was highly expressed after day 20 of birth. Immunofluorescence staining on mixed testicular cells isolated from adult wild-type mice demonstrated that MORN3 was expressed in the acrosome in germ cells throughout spermiogenesis. The protein was also present in the manchette of elongating spermatids. The total MORN3 expression and acrosome localization were not changed in the Meig 1-deficient mice. However, its expression in manchette was dramatically reduced in the mutant mice. Our studies suggest that MORN3 is another regulator for spermatogenesis, probably together with MEIG1.