国境卫生检疫行业标准体系修订初探

目的本文旨在从理论上探讨国境卫生检疫行业标准框架修订的必要性和重要性。方法回顾卫生检疫行业标准的发展和分类,提出对现有行业标准框架的修订意见。结果新的体系框架它对于规范和促进卫生检疫业务、在系统内开展基础培训、培养一专多能型人才起到了应有的作用。结论近十年来卫生检疫面临的形势不断变化,原有的标准体系已经难以适应当前工作需要,亟需进行修订规范。     

T-SPOT与结核菌素实验对结核病患者的临床诊断价值分析

目的分析T-SPOT与结核菌素实验对结核病患者的临床价值。方法选取2015年12月～2017年12月疑似结核病患者202例,经过实验组织学检验100例患者确诊为结核病患者,进行外周血T-SPOT检测和结核菌素实验,比较两组患者诊断结果。结果以最终实验组织学检验为金标准,T-SPOT阳性检出率明显优于结核菌素检出率,差异具有统计学意义(P0.05),T-SPOT监测灵敏度、特异度、准确度均优于结核菌素实验,差异具有统计学意义(P0.05),T-SPOT检测Kappa值为0.985,说明一致性良好,结核菌素实验检测Kappa值为0.512,说明两者一致性中等。结论利用T-SPOT诊断结核病患者,敏感度高且特异性明显,对结核和肺外结核都有很好的应用价值,值得推广应用。     

新药甲磺酸帕珠沙星的手性拆分

目的：建立新药甲磺酸帕珠沙星手性拆分的方法。方法：运用AD手性柱法和流动相添加剂法，考察了不同条件下对分离的影响。结果：运用这两种方法，甲磺酸帕珠沙星都可以达到基线分离，相同条件下，柱温越低，拆分因子越大。结论：在分离甲磺酸帕珠沙星时，流动相添加剂法要优于手性柱法。     

低蛋白饮食对环孢素A肾病大鼠肾间质纤维化的作用

Objective To investigate whether low-protein diet has protective effect on the progression of renal interstitial fibrosis in rats with cyclosporine A (CsA)-induced nephropathy. Methods Eighteen male Sprague-Dawley rats were randomly divided into three groups (6 rats in each group). The rats in control group (C group) received common diet; in model group (M group) low-salt diet; in intervention group (Ⅰ group) low-salt and low-protein diet. After diet adaptation period of one week, the rats in C group received subcutaneous injection of olive oil 1 mg/kg daily for 5 weeks, while M group and Ⅰ group subcutaneous injection of CsA (diluted into 25 g/L with olive oil) 1 ml/kg for 5 weeks. All the rats were sacrificed at the end of the 5th week. The food-intake and body weight were measured daily. The creatinine clearance (Ccr) was examined before rats were sacrificed. The semi-quantitative pathological analysis on kidney sections was performed. The mRNA and protein expression of transforming growth factor-β1 (TGF-βI) and type Ⅰ collagen (Col Ⅰ) in kidney tissue was determined with real time PCR and immunohistochemical staining, respectively. Results The food-intake and body weight of rats in M and I groups were significantly lower than those in C group (P<0.05). Compared with C group, the Ccr levels in M and Ⅰ groups were significantly reduced [(0.65±0.15) ml/min, (0.40+0.13) ml/min vs (1.55±0.29) ml/min, P<0.05], the relative fibrosis areas of kidney interstitium in M and I groups were significantly increased (3.60%±0.46%, 3.26%±0.75% vs 0.44%±0.24%, P<0.05), the mRNA and protein expression of TGF-β1 in M and I group was significantly up-regulated (by 2.6 and 3.1 times in mRNA and by 1.5 and 1.6 times in protein, respectively, P<0.05), and the mRNA and protein expression of Col Ⅰ in M and I groups was also significantly up-regulated (by 3.0 and 3.5 times in mRNA and by 2.3 and 2.1 times in protein, respectively, P<0.05). There were no significant differences between M and I groups in every parameters above-mentioned except the rat body weight and Ccr. Both the body weight and Ccr in Ⅰ group were significantly lower than those in M group (P<0.05). Compared with C group, the urine osmotic pressure in M group and in I group were deceased (for M group, P>0.05; for I group, P<0.05). Compared with C group, the serum cholesterol levels in M and I groups were significantly increased (P<0.05), and the serum phosphorus level in I group was significantly decreased (P<0.05). The levels of serum albumin and serum calcium of all three groups had no statistical differences (P>0.05). Conclusion Low-protein diet has no renoprutective effects on the rat model of cyclosporin A nephropathy, on the contrary, may induce body weight loss.     

脑血管病引起眩晕的临床观察

临床上能引起眩晕的疾病很多,其中因脑血管病变引起的眩晕占大多数.为明确眩晕原因,及时给予病人正确治疗,减轻病人痛苦,笔者将我院自1994-1999年间共收治因脑血管病变引起的眩晕患者100例进行分析总结,报告如下.     

氩激光治疗中心性浆液性脉络膜视网膜病变

中心性浆液性脉络膜视网膜病变（CSR）是临床常见疾病。现将我院2002—12～2005—10行氩激光光凝治疗的23例CSR总结报告如下。     

创伤性膈肌破裂的诊治体会

创伤性膈肌破裂(Traumatic Rupture of Diaphragm,TRD)通常缺乏典型症状并多伴有合并伤,容易延误诊断.一旦诊断与治疗不及时,易导致严重并发症的发生,如创伤性膈疝,危及患者的生命.本文通过对苏北人民医院1987年1月至2004年9月收治的16例TRD患者的诊治过程的回顾性分析,旨在探讨TRD的早期诊断及治疗的方法.     

丝裂霉素C在翼状胬肉切除联合自体结膜瓣移植术中的应用

目的 讨论安全有效浓度下，丝裂霉素C在翼状胬肉切除联合体自体结膜移植术中的应用，观察对翼状胬肉的复发的防治效果。方法 对54例64眼原发性胬肉者施行显微镜下胬肉切除带蒂结膜瓣转位移植联合术中或术后丝裂霉素C（mitomycin C MMC)的应用，经5－12月随诊，与单纯手术组（27例33眼）原发性胬肉术后复发情况作对比分析。结果 复发率要术中用药组为3．33％，术后用药组5．88％，对照组18．18％，经X^2检验，术中、术后用药与对照组 差异有显著性（P＜0．05），而术中或术后应用MMC的复发率差异无性，均可降低胬肉的复发率。结论 适时、适量应用MMC对有效防止翼状胬肉的复发有着非常重要的意义。     

家庭支持与维持性血液透析患者心理健康的关系

目的:调查分析家庭支持与维持性血液透析(MHD)患者心理健康之间的关系。方法:选取武汉大学中南医院肾内科血液净化中心血透时间3个月的MHD患者115例,根据家庭支持量表得分高低,分为高家庭支持组和低家庭支持组,采用症状自评量表(SCL-90)评估两组心理健康状况;比较两组家庭支持得分和SCL-90评分差异,分析家庭支持与患者心理健康状况的相关性。结果:MHD患者高家庭支持组家庭支持总评分(12.28±1.35分)明显高于低家庭支持组(7.00±2.08分),差异有统计学意义(P0.01)。低家庭支持组SCL-90评分在躯体症状不适(躯体化)、强迫症状、抑郁、焦虑、敌对、恐怖、偏执、精神病性表现等方面均比高家庭支持组严重,对人际关系也较敏感(P0.05)。两组家庭支持度得分与SCL-90的9大因素(躯体化、强迫、人际关系敏感、抑郁、焦虑、敌意、恐怖、偏执、精神病性表现)积分均呈明显负相关(P0.05)。结论:家庭支持直接影响MHD患者的心理健康。