神经生长因子基因转染联合强化铁营养防治豚鼠爆震性聋的实验研究

Objective To study the protective effects of adenovirus-mediated human beta-nerve growth factor gene (hNGFβ) transfer combined with iron fortified nutrition on blast hearing damage in guinea pigs. Methods Deafness was induced by blast (172dB SPL) in 35 healthy guinea pigs. Seven days after noise exposure, 10 guinea pigs were inoculated with adenovirus-mediated hNGFβ (Ad-hNGFβ) into the perilymphatic space (the gene group), another 10 guinea pigs were given hNGFβ combined with iron fortified nutritional diet (the combination group), still another 10 guinea pigs were inoculated with artificial perilymphatic fluid (APF), and 5 guinea pigs served as control, given neither medicine nor noise exposure. Auditory brainstem response (ABR) threshold shifts were monitored in the guinea pigs before blast exposure and after gene transfer. Changes in cochlear morphology were examined with immunohistochemical and HE staining. Results One week after successful inoculation of hNGFβ, it was noted that Ad-hNGFβ protein was expressed in each turn of the cochlea, and its intensity was almost equal. After gene transfer in the combination group and the gene group, ABR threshold shifts at week 1 and 4 were significantly smaller than those of the APF group. HE staining showed that the number of the spiral ganglion cells in the combination group and the Ad-hNGFβ group was significantly higher than that of the control group after 4 weeks (P<0.01). Conclusions Ad-hNGFβ combined with iron fortified nutritional diet was effective in the prevention and treatment of blast hearing damage of cochlear hair cells.     

褪黑素受体基础与临床研究

褪黑素(melatonin，Mel)是松果体分泌的一种神经内分泌激素。松果体分泌Mel具有昼夜节律，由下丘脑视交叉上核控制，与自然界的光暗周期的变化合拍，这种节律性波动表现为夜间达到高峰而白昼降至谷值。除了传统认为的调节昼夜节律和季节性节律等生理性节律的作用以外，目前大量的研究显示Mel还具有其他广泛而重要的生理、药理功能，包括调节生殖系统、免疫系统、神经系统、心血管系统的功能，如：抑制多种生物的生殖功能，增强生物免疫系统功能，并能镇静、安神、催眠，对人体的血压等也有一定的调节作用。     

唐山市2001年肾综合征出血热监测

根据《流行性出血热监测工作计划》要求 ,2 0 0 1年 3～ 4月和 10～ 11月对唐山市所辖丰润县沙流河镇张庄子村、韩城寨子村 ,古冶区大庄坨乡的新庄子村和大庄坨村 ,滦县茨榆坨乡兴隆庄、油榨村、东兴坨、三山院村、松树营村、西老里村作为监测点 ,进行了鼠密度、鼠类种群及其携带汉坦病毒 (HV )抗原、抗体和健康人群隐性感染监测 ,现将结果报告如下。1　材料和方法1.1　人间疫情监测　对辖区内报告的肾综合征出血热(HFRS)病例 ,进行个案调查 ,核实诊断 ,疫区处理。1.2　鼠密度及鼠种群监测　对上述试点室内 (滦县仅室内 )、野外按规定用 …     

Adenovirus-mediated human β-nerve growth factor gene transfer has a protective effect on cochlear spiral ganglion after blast exposure

Objective：To study whether adenovirus-mediated human β-nerve growth factor （Ad-hNGFβ） gene has any protective effect on blast hearing impairment. Methods：Deafness was induced by blast exposure （172.0 dB） in 30 healthy guinea pigs. On day 7 of blast exposure, Ad-hNGFβ was infused into the perilymphatic space of 20 animals as the study group （hNGFβ group）, and artificial perilymph fluid （APF） was infused into the perilymphatic space of the other 10 animals as the control group. At weeks 1, 4 and 8 after blast exposure, the animals were sacrificed and the cochleae were removed for immunohistochemical and HE stainings. Results： Expression of Ad-hNGFβ protein was detected in each turn of the cochlea at the 1st week, with almost equal intensity in all turns. At the 4th week, the reactive intensity of the expression of Ad-hNGFβ protein decreased. At the 8th week, no expression was detectable. The results of HE staining showed that the amount of spiral ganglions in hNGFβ group was significantly greater than that of the control group at week 4 （P〈0.01）. Conclusion： Ad-hNGFβ can be expressed at a high level and for a relatively long period in the blast impaired cochlea, suggesting that Ad-hNGFβ has a protective effect on cochlear spiral ganglion cells after blast exposure and the efficient gene transfer into cochlea had been achieved without toxicity.     

呼吸道合胞病毒疫苗研究进展

呼吸道合胞病毒(RSV)是全球婴儿严重下呼吸道疾病最重要的病原体，发展其疫苗已被WHO列为全球最优先发展疫苗之一。目前RSV疫苗研究主要集中于亚单位疫苗、减毒活疫苗、DNA疫苗等。     

汉族成人部分外周组织褪黑素受体分布及意义

褪黑素 (melatonin ,Mel)具有广泛的生物学效应 ,但其在生物体内作用的靶器官未完全明确 ,阻碍了对它作用机制的进一步研究。本实验室研究显示人胚胎甲状腺、肾上腺、心血管等组织均存在MR。因而 ,明确外周组织是否存在MR ,对于研究Mel在更为广泛的生物学作用及其机制具有重要意义。目的 :证实汉族成人骨骼肌和皮肤等外周组织存在褪黑素受体 (mela toninreceptor,MR) ,并研究MR亚型及其亚细胞分布。方法 :所有组织均取自健康志愿者。用免疫组织化学和逆转录 -多聚酶链反应 (RT -PCR)产物基因测序等方法证实人骨骼肌组织存在MR及其亚…     

人胚胎肾上腺皮质褪黑素受体亚型蛋白的分布

目的：研究褪黑素（Mel)受体亚型在人胚胎肾上腺皮质的分布。方法：应用免疫组化染色检测Ｍel受体亚型的人胚胎肾上腺皮质的分布。结果：在人胚胎肾上腺皮质的球状速、束状带和网状带均存在Ｍel受体mt1(Mella)和ＭＴ２（Ｍellb)亚型蛋白的表达,且主要分布于细胞质。结论：人胚胎肾上腺皮质存在Ｍel受体mt1和ＭＴ２亚型。     

溃结康激活自噬及调节肠道菌群防治溃疡性结肠炎的作用机制研究

目的:探讨溃结康调控自噬及肠道菌群防治溃疡性结肠炎的作用机制。方法:采用葡聚糖硫酸钠(Dextran sulphate sodium, DSS)制备急性溃疡性结肠炎(Ulcerative colitis, UC)小鼠模型,随机分为正常对照组、模型对照组、柳氮磺胺吡啶0.45 g/kg组、溃结康3.2、6.4、12.8 g/kg组。除正常对照组外,各组小鼠自由饮用3%DSS水溶液7 d,隔天更换新鲜DSS水溶液。每天观察各组小鼠体质量及疾病活动指数变化,并于第8 d试验结束时,脱颈椎处死各组小鼠收集结肠和粪便;HE染色观察结肠组织病理学改变;电镜检测结肠上皮损伤及自噬体形成数量;RT-qPCR及Western-blot法检测结肠微管相关蛋白轻链(Lc3)、苄氯素1(Beclin1)、自噬蛋白(P62)mRNA及蛋白表达;16S核糖体RNA(16S rRNA)测序技术检测小鼠粪便肠道菌群的变化。结果:与正常对照组比较,模型对照组小鼠体质量显著下降、DAI评分显著升高,结肠长度显著降低,结肠黏膜损伤严重,病理CMDI评分显著升高(P<0.01),视野内未见自噬小体,结肠组织中Lc3、B...     

胃癌及其淋巴转移灶β1,β4亚单位整合素表达的临床意义

胃癌及其淋巴转移灶β１、β４亚单位整合素表达的临床意义许洪卫何金王元和陈泳莲一、材料与方法１．标本采集与处理：２９例手术切除新鲜标本取自１９９４年６月至１９９５年５月间住入我院普通外科的胃癌患者，每例包括原发灶、距癌边缘５ｃｍ以上的邻近正常胃粘膜和可...     

胃癌中医证型与胃癌转移相关基因E-cadherin的关系研究

目的:从基因蛋白表达上探索胃癌患者中医证的本质.方法:将收集到的术前胃癌患者病例资料按中医辨证分型标准确定其证型归属;用免疫组化EnVision二步法检测术后胃癌肿瘤标本中E-cadherin基因蛋白表达情况.结果:E-cadherin在100例胃癌患者中的阳性表达率为90%,证型间表达差异存在显著性(P<0.01);进一步两两比较示,痰湿凝结型、气血双亏型与脾胃虚寒型之间无明显差异,表达较高;瘀毒内阻型与肝胃不和型无明显差异,表达较低.结论:瘀毒内阻型与肝胃不和型胃癌患者的E-cadherin表达偏低,此两种证型肿瘤转移形成的途径可能与E-cadherin,即与肿瘤细胞间同质性黏附力降低相关.