3种麻醉剂对兔Oddi括约肌肌电运动的影响

目的:研究麻醉剂速眠新、氯胺酮、戊巴比妥钠对兔Oddi括约肌(SO)肌电运动的影响。方法:取家兔24只,采用改良的Thomas法间置空肠建立SO肌电运动检测通道,术后10d时将存活的16只随机分为3组,分别静脉注射速眠新(n=6)、氯胺酮(n=5)、戊巴比妥钠(n=5),在清醒状态及麻醉起效后5min各组耳缘静脉注射胆囊收缩素(CCK)20、100ng.kg-1(2次给药至少间隔3min),期间连续记录3种麻醉剂给药前、后及CCK给予后的SO肌电运动,以运动指数及其增幅、兴奋效应持续时间等指标表示,同时以未加麻醉剂时给予CCK前、后的肌电运动为对照。结果:与给予麻醉剂前比较,速眠新和氯胺酮组给药后SO肌电运动指数明显增加(P<0.05),而戊巴比妥钠组变化不明显;与对照组比较,CCK使速眠新和氯胺酮组SO肌电运动指数增幅降低,戊巴比妥钠组运动指数增幅增加、兴奋效应持续时间延长(P<0.05)。结论:3种麻醉剂对兔SO肌电运动均有影响,以速眠新和氯胺酮的影响较大,戊巴比妥钠影响相对较小。     

不同敷料在糖尿病足溃疡伤口治疗中的研究与应用

BACKGROUND: To date, various types of dressings have been proved to promote healing of diabetic foot ulcers. OBJECTIVE: To introduce the principle and application of various kinds of wound dressing as well as other adjuvant therapies for diabetic foot ulcers. METHODS: CNKI, PubMed, Medline and Foreign Medical Journal Full-Text Service were retrieved by computer for relevant literatures published from 2005 to 2015. The search terms were “diabetic foot ulcer, wound healing, dressing, treatment” in Chinese and English, respectively. RESULTS AND CONCLUSION: The most commonly used dressings for diabetic foot ulcers include gels, hydrogels, sponges, foam dressings, film dressings. Polymer materials with different components exhibit their unique chemical, physical and biological properties, such as natural polymer dressings (chitin, chitosan and its derivatives, fibroin, dextran), synthetic polymer dressings (polyvinyl alcohol, polyethylene glycol/polyethylene oxide, poly(α-ester)s, all of which can promote the healing of diabetic foot ulcers. Other therapies for diabetic foot ulcers include herbal therapy, becaplermin, and maggot therapy.     

清醒状态下兔Oddi括约肌肌电活动检测模型的建立

目的：建立清醒状态兔Oddi括约肌（SO）肌电检测的动物模型,研究其肌电活动特征。方法：将成年家兔麻醉后,于胆囊底部置管一根;将十二指肠大乳头对系膜缘与间置的空肠袢远端吻合,空肠袢关闭后与胆囊内置管分别埋置于皮下,恢复14d后用环状电极进行清醒状态下SO肌电检测。对其消化间期、进食后、外源性胆囊收缩素（CCK）泵注下SO肌电幅值、频率及每分钟运动指数进行分析。结果：利用该模型可以在清醒状态下稳定记录到SO肌电活动,表现为慢波恒定基础上快波的周期性变化,进食后SO的快波增强,外源性CCK对兔SO的肌电有兴奋作用。结论：清醒状态下SO肌电活动检测更能反映括约肌的功能状态,这为今后研究病理状态下SO的活动提供了技术平台。