儿童髓母细胞瘤中VEGFR-2的表达

目的：探讨血管内皮生长因子受体VEGFR－2在儿童髓母细胞瘤中的表达及临床意义。方法：采用免疫组化LSAB法检测84例获随访的儿童髓母细胞瘤中的VEGFR－2表达，按术后生存期3，5，10年分为A，B，C三组，使用Cox回归统计分析。结果：84例儿童髓母细胞瘤中，VEGFR－2阳性表达74例（88．09％），A，B，C三组间VEGFR－2阳性表达分别为100％，88．89％，55．33％（P＜0．01），Cox回归分析显示VEGFR－2是影响生存时间的一个独立的预后因子，它与预后存在负相关关系。结论：VEGFR－2表达水平可作为儿童髓母细胞瘤的预后指标之一。     

垂体脓肿

垂体脓肿是一种罕见但死亡率极高的疾病,死亡率为28％,若合并脑膜炎则为45％。至1996年只有约50例报道。在抗生素应用以前,大多数是在脑膜炎或败血症病人尸体解剖中发现垂体脓肿,很难术前确诊。近年来国内亦有相应报道。为提高对本病的认识,本     

pSVPoMcat微基因修饰雪旺细胞移植对损伤脊髓功能恢复的影响

目的观察pSVPoMcat微基因修饰雪旺细胞(SC)移植对损伤脊髓功能恢复的影响. 方法采用切割法制备脊髓半横断损伤模型(T8平面),实验动物随机植入pSVPoMcat微基因的 SC组(A组)、SC组(B组)和明胶海绵组(C组),每组10只.用皮层体感诱发电位(CSEP) 及图像分析方法比较各组间诱发电位潜伏期、波幅,再生轴突计数.同时,行电镜观察及联合行为(CBS)记分. 结果 A,B两组潜伏期和波幅呈恢复趋势,且A组已接近正常,与CBS结果一致.再生轴突计数A组为388±163,B组为109±16(P＜0.01),与电镜观察一致. 结论 pSVPoMcat微基因修饰SC移植对脊髓损伤(SCI)后的神经功能恢复有明显的促进作用.     

pSVPoMCAT微基因修饰雪旺氏细胞移植治疗实验性脊髓损伤的酶及免疫组化评价

Genetherapyofspinalcordinjury（SCI）remainsintheexper－imentalstage．Now，neurotrophicfactortomodifySchwanncell（SC）orfibroblastsareimplantedintoSCIregiontoobservetheregenera－tionsofaxons犤１犦．WefirstbuildPo－５＇－flankinginducedmicrogeneforbasicproteinofmyelinsheathwhichistransientlynamedaspSVPoMcat．ThepSVPoMcatgenewasintroducedintoSCthroughcationliposome．ItwasprovedthatpSVPoMcatcouldenhancefunc－tionofSCandelongateitssurvivalperiodinvitro犤２犦．pSVPoMcatwasimplantedintoS…     

Repair effect of Schwann cells modified by microgene pSVPoMcat on injured spinal cord in rats

OBJECTIVE: To observe the repair effect of Schwann cells (SCs) modified by microgene pSVPoMcat on injured spinal cord in rats. METHODS: Semi-transection injury at the level of T(8) of spinal cord was made with cutting method on 120 Sprague Dawley (SD) rats. Then 40 rats implanted with SCs modified by microgene pSVPoMcat were taken as Group A, 40 rats implanted with simple SCs as Group B and the other 40 rats were taken as the control group (Group C). The functional recovery of the rats was observed through combined behavioral score (CBS) and cortical somatosensory evoked potential (CSEP), and the expression of the glial fibrillary acidic protein (GFAP) was measured with in situ hybridization and immunocytochemistry. At 3 months after operation, the rats were examined with magnetic resonance image (MRI), and the neurofilaments (NF) of the axons were stained with immunohistochemical method. RESULTS: GFAP expression in Group A was significantly lower than that of the other 2 groups. MRI showed that the spinal signals in the injured area recovered fundamentally in Group A, didn't recover in Group B and malacia focus was found in Group C, which was same as the results of NF staining. Wave amplitudes in incubation periods in Group A and Group B tended to recover. It recovered to the normal level in Group A, which was similar to the results of CBS. CONCLUSIONS: SCs modified by microgene pSVPoMcat can inhibit GFAP expression, improve the growth of the axons and the functional recovery of neurons after spinal cord injury.     

股部巨大淋巴水囊肿1例报告

股部巨大淋巴水囊肿１例报告赵振波，朴永旭患儿，男，１岁。出生时见左股内侧有一鹅卵大肿块，无痛。此后该包块逐渐长大，于１９９１年１１月７日以“血管瘤”收入院。查体：患儿发育正常，于左大腿内侧由膝至腹股沟区见一１２ｃｍＸｇｃｍ之肿块，其周界清楚，不红，无...     

儿童髓母细胞瘤Bcl-2基因表达的研究

目的 检测髓母细胞瘤的凋亡情况,并分析其与肿瘤恶性增殖和预后的关系。方法 以84例获随访的儿童髓母细胞瘤组织（按术后生存期<3年、3-5年、5-10年分为A、B、C3组）为研究对象,用原位杂交及免疫组化染色法分别检测Bcl-2 mRNA和增殖细胞核抗原（PCNA）的表达,并用3末端标记法做原位细胞凋亡检测。结果 72例（85.7％）表达Bcl-2 mRNA,A、B、C组阳性率分别为100％、88.9％、40％,彼此之间阳性率差异显著（P<0.05）。PCNA阳性细胞密度随Bcl-2 mRNA表达增强而增高,而凋亡细胞密度随Bcl-2 mRNA表达增强而减少。结论 儿童髓母细胞瘤中 Bcl-2基因高表达可抑制其凋亡,这可能是瘤细胞恶性增长的重要原因之一。     

儿童外伤性脑梗塞（附21例报告）

儿童外伤性脑梗塞临床上比较少见,其发病机理和临床特点还不完全清楚,易误诊误治。我院自1988年至1996年共收治21例,占同其收治1286例颅脑损伤的1.63％,现报告分析如下。 临床资料 一、一般资料 男13例,女8例。年龄10月至9岁,平均3.2岁。坠落伤11例,摔伤7例,打击伤2例,牛顶伤1例,均为闭合性颅脑损伤。枕部着力13例,颞顶部着力3例,额部着力1例,其余不详。 二、临床表现所有病例均表现为外伤后肢体瘫痪,出现时间0～28小时不等,进行性加重。伤后浅昏迷3例;一过性意识丧失12例;无     

垂体脓肿

垂体脓肿是一种罕见的疾病，至1996年为止英文文献只有约50例报道。在抗生素应用以前大多数是在脑膜炎或败血症病人尸检中发现此病，很难术前确诊。Domingue等1977年报道了第一例术前确诊的垂体脓肿。近年来国内亦有少数病例报道。其病死率为28%；若合并脑膜炎，则高达45%。本文对此病进行综述。