Additive effects of earthworm extract with HpD-laser on the enhancement of reactive oxygen production and the inhibition of[~3H]—thymidine.uptake in mouse ascitic tumor cells

The effects of combined use of earthworm extract(912)and HpD-laser on the produc-tion of reactive oxygen and the biosynthesis of DNA in S_(180) tumor cells were studied throughchemiluminescence measurement and[~3H]-TdR incorporation assay.The results showed that as com-pared with the control,the intensity of chemiluminescence emitted by tumor cells treatedsimultaneously with 912 and HpD-laser was enhanced more than ten-folds,while that treated with912 or HpD-laser alone was increased only 2～4 folds.The[~3H]-TdR incorporation into tumorcells of the former group was inhibited upto 74.1%,and that of the latter groups decreased onlyby 42.2% and 40.0%,respectively.In accordance with these biochemical changes,the ultrastructuraldamage of tumor cells of the former,combinedly treated group appeared to be the most serious.This suggests an additive effect of 912 with HpD-laser on tumor cells.In addition,if free radicalscavengers,such as catalase and superoxide dismutase,were added to the reaction systembefore chemiluminescence assay,the luminescent enhancement effect mentioned above was dramaticallyalleviated,implying the presence of O_2~ and H_2O_2 in the system.Therefore,as to the toxic effecton tumor cells,912 and HpD-laser are not only additive in efficiency,but also similar in theunderlying mechanism of action.     

胃癌细胞选择性阿霉素免疫脂质体的制备及其特性

免疫脂质体(immunoliposome)系脂质体与特异性抗体连接而成,具有普通脂质体和导向药物二者的优点。其中的药物活性保持好,携药量大,并能与靶细胞多价结合,因而在肿瘤导向治疗领域日益受到重视。 作者采用本室制备的胃癌单克隆抗体MGb_2经NHSP修饰引入软脂酸,逆相蒸发法制备脂质体,同时包入阿霉素(adriamycin)。用去氧胆酸透析法将修饰抗体插到阿霉素脂质体表面,经过Scpharose 4B柱层析纯化得到免疫脂质体。同     

大枣叶提取物的抗炎作用及其机理

本文首次报道了大枣叶提取物对于甲苯所致小鼠耳廓炎症、蛋清性大鼠足跖肿胀和棉球致肉芽组织增生有显著的抑制作用。且能减轻幼年大鼠胸腺重量，使肾上腺重量增加。并可明显清除由鲁米诺介导的黄嘌呤─—黄嘌呤氧化酶产生的Ｏ２－及由Ｆｅ２＋导的Ｈ２Ｏ２产生的ＯＨ－。表明大枣叶提取物具有抗炎作用，且可能与其对自由基的清除作用有关。     

DDC提高纳米级阿霉素磺油乳剂抗肿瘤作用的实验研究

目的：探讨二乙基二硫代氨基甲酸酯（DDC）对提高纳米级阿霉素碘油乳剂在体内外的抗肿瘤作用。方法：用MTT试验评价纳米级阿霉素碘油乳剂对耐药细胞及肿瘤细胞的杀灭作用，用SD大鼠建立Walk-256肝癌模型，通过肝动脉分别注入生理盐水、阿霉素、纳米级阿霉素碘油乳剂，DDC加纳米级阿霉素碘油乳剂以及DDC加阿霉素脂质体碘油乳剂，分别测定各组的肿瘤生长率和小鼠生命延长率。结果：经过DDC预处理后，阿霉素对阿霉素耐药肿瘤细胞SGC7901／CVR和SGC790／WT的IC50（μg/ml）分别从原来的18．40降至0．74和4．00降至0．32。未经DDC处理过的各组平均肿瘤生长率远高于预先用DDC处理过的各组（P＜0．01）。而小鼠生命延长率则明显低于预先用DDC处理过的各组（P＜0．05）。结论：用DDC预先处理抑制肿瘤细胞中的超氧化物歧化酶（SOD）可提高阿霉素的抗肿瘤作用。     

活性氧的生成与清除失调导致克山病的假设

由于活性氧的生成与清除失调导致克山病的假设,是根据我国广大医务工作者、科研工作者经过长期研究而取得的大量资料,并结合国外有关活性氧研究的一些资料,进行分析、综合而提出的。 作者在1978年提出此假设后,曾向有关单位征求意见、提出建议。并在陕西省化学会1979年年会的大会上作《自由基在生物体系中的作用》专题报告时作了介绍,后又在《活性氧及其生物学作用》(生物化学与生物物理进展1981年第2期第23—29页)一文中作了概述。     

抗氧化剂药膜对烧伤后继发性损伤的影响

抗氧化剂药膜对烧伤后继发性损伤的影响孙淑芬莫简王多宁骆文博施炯苏映军王吉村郭正仁刘健贺诗华林英华烧伤后继发性氧化损伤可能与自由基介导的脂质过氧化有关，超量自由基引起的氧化应激反应具有细胞毒作用，使创面进一步加深，会影响创面的愈合。这一过程一般发生在烧...     

Protection of retina from lipid peroxidation with super''''oxide dismutase

Our previous experiments demonstrated the occurrence of retinal lipidperioxidation following vitreous hemorrhage.In the present study,0.2ml ofautologous whole blood was injected into the vitreous of both eyes in rabbits.One eye of each rabbit received an additional injection of 0.2ml of superoxidedismutase (SOD,794 U);the other eye served as a control.The malondialdehyde(MDA) in the retina was assayed with fluorescence spectrophotometry.The resultsshowed that the MDA was significantly lower in eyes injected with SOD (0.047±0.010 nmol/mg) than in control eyes (0.096±0.011 nmol/mg,P<0.05) 10d afterinjection.Ultrastructural examination revealed that the photoreceptor cells of theeyes with SOD injection were relatively normal.It is suggested that SOD can pro-tect the retina from lipid peroxidation.     

化学发光法测定两种铜（Ⅱ）配合物歧化超氧阴离子的活性

化学发光法测定两种铜（Ⅱ）配合物歧化超氧阴离子的活性王多宁，莫简，赵喜荣，孙淑芬，郭正仁（化学教研室西安７１００３３）关键词化学发光；配合物；超氧阴离子中图号Ｏ６５７．３９自１９６９年ＭｃＣｏｒｄ等人发现超氧化物歧化酶（ＳＯＤ）以来，国内外都从提取方...