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The effects of combined use of earthworm extract(912)and HpD-laser on the produc-tion of reactive oxygen and the biosynthesis of DNA in S_(180) tumor cells were studied throughchemiluminescence measurement and[~3H]-TdR incorporation assay.The results showed that as com-pared with the control,the intensity of chemiluminescence emitted by tumor cells treatedsimultaneously with 912 and HpD-laser was enhanced more than ten-folds,while that treated with912 or HpD-laser alone was increased only 2~4 folds.The[~3H]-TdR incorporation into tumorcells of the former group was inhibited upto 74.1%,and that of the latter groups decreased onlyby 42.2% and 40.0%,respectively.In accordance with these biochemical changes,the ultrastructuraldamage of tumor cells of the former,combinedly treated group appeared to be the most serious.This suggests an additive effect of 912 with HpD-laser on tumor cells.In addition,if free radicalscavengers,such as catalase and superoxide dismutase,were added to the reaction systembefore chemiluminescence assay,the luminescent enhancement effect mentioned above was dramaticallyalleviated,implying the presence of O_2~ and H_2O_2 in the system.Therefore,as to the toxic effecton tumor cells,912 and HpD-laser are not only additive in efficiency,but also similar in theunderlying mechanism of action. 相似文献
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目的:探讨外源性一氧化氮(NO)对人胚胎神经干细胞(NSCs)增殖的抑制作用.方法:取4月龄(±15d)正常孕妇引产胎儿大脑皮质组织分离代NSCs,免疫荧光化学法鉴定.培养基中加入不同浓度的硝普钠后,Griess还原法检测培养液中NO氧化产物的含量;MTT法测定细胞活力.结果:加入不同浓度硝普钠24h后,培养液中NO氧化产物的含量随硝普钠浓度的增高而增加,且显著高于对照组.MTT法显示经硝普钠作用后的NSCs活细胞数较对照组明显减少,并呈浓度相关性;在去除硝普钠后继续培养的NSCs仍可保持增殖能力.结论:外源性NO对培养状态下的人胚胎NSCs增殖有抑制作用. 相似文献
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血浆同型半胱氨酸水平及临床诊断意义 总被引:3,自引:0,他引:3
同型半胱氨酸血症与许多疾病有关,血中tHcy升高可致流产、新生儿缺陷直至中风、老年性痴呆及其它老年性疾病如骨质疏松及老视,尤其被认为tHcy升高是冠心病的一个独立危险因素,而高同型半胱氨酸血症可能与同型半胱氨酸代谢有关的酶和维生素缺乏有关.作者就同型半胱氨酸的代谢、血浆中同型半胱氨酸水平及在临床诊断中的意义进行综述. 相似文献
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目的探讨NADPH氧化酶来源的活性氧(ROS)在TNF-α诱导乳鼠心肌细胞肥大中的作用。方法采用体外乳鼠心肌细胞培养,给予TNF-α、APO单独或联合作用细胞24h,以BCA法测定心肌细胞蛋白合成,共聚焦显微镜测量心肌细胞ROS含量,RT-PCR法测定心肌细胞心钠素ANP mRNA的表达。结果 TNF-α可明显增加心肌细胞蛋白合成,心肌细胞体积增大,ROS生成增多,ANP mRNA表达水平显著增加,以上作用可被APO明显抑制。结论TNF-α通过增加心肌细胞NADPH氧化酶来源的ROS生成,促进心肌细胞肥大。 相似文献
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Our previous experiments demonstrated the occurrence of retinal lipidperioxidation following vitreous hemorrhage.In the present study,0.2ml ofautologous whole blood was injected into the vitreous of both eyes in rabbits.One eye of each rabbit received an additional injection of 0.2ml of superoxidedismutase (SOD,794 U);the other eye served as a control.The malondialdehyde(MDA) in the retina was assayed with fluorescence spectrophotometry.The resultsshowed that the MDA was significantly lower in eyes injected with SOD (0.047±0.010 nmol/mg) than in control eyes (0.096±0.011 nmol/mg,P<0.05) 10d afterinjection.Ultrastructural examination revealed that the photoreceptor cells of theeyes with SOD injection were relatively normal.It is suggested that SOD can pro-tect the retina from lipid peroxidation. 相似文献
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