匹罗卡品致(癎)大鼠海马中表达生长抑素的中间神经元数目变化及其轴突出芽

Objective To investigate the roles of somatostatin(SS)positive intemeurons in the development and compensation of temporal lobe epilepsy.Methods Piloearpine-induced epilepsy rat model was established.Immunohistochemistry method was used to detect number changes and axonal sprouting of SS positive intemeurons in different domains of the hippocampus at difierent time points.Degeneration of SS positive interneurons and their neurophils were detected by the double immunofluorescence staining with SS and Fluoro-Jade B(FJB)at 7 and 60 days after status epilepticus (SE).Results In the exoerimental rat group,the number of SS positive neurons decreased in each hippocampal domain,and it reached the lowest at 7 days post-SE(There were 11.1±3.3 in hilus,2.8±0.9 in CA1region and 1.8±0.7 in CA1region,t=13.519,9.644 and 8.808,all P<0.01).In chronic phase,the number of SS neurons gradually recovered,and exceeded the control group in CA1 area at 60 days post-SE(12.8±1.5 vs 8.8±1.3,t=-4.506,P<0.01),however,the number of SS neurons in the hilus(25.5±4.6)and CA1 area(4.8±0.8)remained significantly less than normal levels(t value were 4.691 and 3.953.both P<0.01).Increased SS positive fibers were found in the lacunosum-molecular (1m)layer and outer molecular layer of dentate gyrus after 30 days post-SE,and numerous SS positive fibers were seen threnghout the layers of area CA1 at 60 days post-SE.Double immunofluuorescence revealed that a few SS positive interneurons and fibers were also labeled by FJB in area CA1 at 7 days post-SE and in CA domain/hilus at 60 days post-SE.Conclusions SS intemeurons loss plays an important role in the development of temporal lobe epilepsy.The loss is partially caIlsed by the degeneration and death of neurons;SS positive neurophils increase within area CA1 in chronic phase may play a significant role in the generation and compensation of temporal lobe epilepsy.     

匹罗卡品致(癎)大鼠海马中表达生长抑素的中间神经元数目变化及其轴突出芽

Objective To investigate the roles of somatostatin(SS)positive intemeurons in the development and compensation of temporal lobe epilepsy.Methods Piloearpine-induced epilepsy rat model was established.Immunohistochemistry method was used to detect number changes and axonal sprouting of SS positive intemeurons in different domains of the hippocampus at difierent time points.Degeneration of SS positive interneurons and their neurophils were detected by the double immunofluorescence staining with SS and Fluoro-Jade B(FJB)at 7 and 60 days after status epilepticus (SE).Results In the exoerimental rat group,the number of SS positive neurons decreased in each hippocampal domain,and it reached the lowest at 7 days post-SE(There were 11.1±3.3 in hilus,2.8±0.9 in CA1region and 1.8±0.7 in CA1region,t=13.519,9.644 and 8.808,all P<0.01).In chronic phase,the number of SS neurons gradually recovered,and exceeded the control group in CA1 area at 60 days post-SE(12.8±1.5 vs 8.8±1.3,t=-4.506,P<0.01),however,the number of SS neurons in the hilus(25.5±4.6)and CA1 area(4.8±0.8)remained significantly less than normal levels(t value were 4.691 and 3.953.both P<0.01).Increased SS positive fibers were found in the lacunosum-molecular (1m)layer and outer molecular layer of dentate gyrus after 30 days post-SE,and numerous SS positive fibers were seen threnghout the layers of area CA1 at 60 days post-SE.Double immunofluuorescence revealed that a few SS positive interneurons and fibers were also labeled by FJB in area CA1 at 7 days post-SE and in CA domain/hilus at 60 days post-SE.Conclusions SS intemeurons loss plays an important role in the development of temporal lobe epilepsy.The loss is partially caIlsed by the degeneration and death of neurons;SS positive neurophils increase within area CA1 in chronic phase may play a significant role in the generation and compensation of temporal lobe epilepsy.     

氟乙酰胺中毒儿童的急救护理

氟乙酰胺类毒鼠药为有机氟杀虫剂.又名敌蚜胺,氟素儿,无味,误服后经消化道吸收而引起中毒.进入机体后主要作用于中枢神经、消化、心血管系统.临床表现为：恶心、呕吐、腹痛、腹泻.重者除上述症状,还有呼吸困难、心力衰竭、神志不清、大小便失禁、反复抽搐、消化道出血、昏迷等.     

红藤败酱液保留灌肠、肠胃宁片口服治疗慢性结肠炎疗效分析

2001年2月-2004年2月。笔者于本院门诊对患慢性结肠炎80例。经红藤败酱液保留灌肠、肠胃宁片口服治疗取得了较好的疗效。现报告如下。     

塞来昔布对人鼻咽癌CNE-2细胞生长抑制及放射增敏研究

Objective To investigate the growth inhibition and radiosensitization of Celecoxib in hu-man nasopharyngeal carcinoma cell line CNE-2. Methods CNE-2 growth inhibition by Celecoxib was eval-uated by MTT method. Apoptosis-related changes in morphology were observed by transmission electron mi-croscopy (TEM). Cell cycle distribution and apoptosis rate were measured by flowcytometry (FCM). The ex-pression of COX-2 protein was observed by SP method after the treatment of Celecoxib. Cells were randomly planted into four groups: irradiation control(Ci), drug group(Cd), irradiation group(R), and Celecoxib plus irradiation group(D+R). Single irradiation of 2,4,6,8,and 10 Gy were administered for colonogenic assay. Cell cycle distribution and apoptosis rate were analyzed at 6 Gy irradiation. Results The growth of CNE-2 cell was inhibited by celecoxib in a dose-and time-dependent manner, the IC50 was 80 μmol/L After the treatment, cell ratio of GO and G, phases was increased (47.03±2.76 vs 56.17±1.95, t=4.68, P= 0.010), whereas the ratio of S and G2/M phases was decreased (33.07±1.86 vs 24.87±1.76, t=5.54, P = 0.010; 19.30±0.53: 17.73±0.83, t=2.75, P=0.050), and the apoptosis rate was increased (1.57±0.47:10.47±0.31, t = 27.39, P = 0.000) in a dose-dependent manner. Apoptosis with nuclear chromatin condensation, fragmentation and cell shrinkage was found by TEM. SP method showed that Celeib decreased COX-2 expression (17.48±0.34 vs 12.82±0.51,t=13.20,P =0.00). The sensitivity ratio(D0) was 1.15. FCM showed that the percentage of cells in G2/M phase was significanty more in R and D+R groups than in Ci and Cd groups (68.00±1.65,54.27±5.74,17.60±0.80,14.86±1.23, t=47.70,P=0.000; t=11.63, P=0.000), and also significantly different between R group and D + R group (t=3.99, P= 0.020). The apoptosis rate was higher in R and D + R groups than Ci and Cd groups(4.83±0.97,9.50± 1.35,1.33±0.86 and 2.28±0.42,t=4.67,P=0.010;t=8.81, P=0.000), D + R group than R group(t =4.85,P=0.010). Conclusions Celecoxib can markedly inhibit the growth and induce apoptosis in CNE-2 cells,which may depend on COX-2 pathway. Celeeoxib potently enhances the radiosensitivity of CNE-2 cells,which may due to the repair inhibit of radiation-induced DNA damage, inhibit of cell proliferation,and enhancement of cell apoptosis after irradiation.     

肿瘤干细胞标记CD44和CD133在鼻咽癌细胞株中的表达检测

目的 探索肿瘤干细胞标记物CD44和CD133在鼻咽癌(NPC)中的表达量及其有效分选方式.方法 常规培养SUNE-1 5-8F细胞,采用免疫荧光技术及流式细胞学技术检测SUNE-1 5-8F细胞中CD44、CD133的表达,并用流式细胞仪分选CD44+、CD44+CD133+细胞.结果 激光共聚焦镜下鼻咽癌SUNE-...     

脂联素、瘦素及可溶性瘦素受体对乳腺癌发病风险影响的人群研究

目的 探讨脂联素、瘦素、可溶性瘦素受体(soluble leptin receptor,sOB-R)对女性绝经前后乳腺癌的单独或联合效应,为揭示肥胖与乳腺癌之间的分子机制提供证据.方法 序贯纳入乳腺癌新发患者469例及同期按1:1年龄频数匹配的469例健康女性为研究对象.采用问卷收集研究对象基线信息,并采用ELISA法...     

小鼠原代血管平滑肌细胞改良分离方法的建立

目的：建立方便、快速、高效的小鼠原代血管平滑肌细胞（vascular smooth muscle cell,VSMC）的分离培养方法,为相关研究提供快速获取原代VSMC的方法技术。方法：分离小鼠主动脉,用I型胶原酶消化血管组织去除内皮细胞等杂细胞;将血管切成1mm。大小组织块种植于六孔细胞培养板孔底,用分离培养液诱导原代VSMC细胞的繁殖生长。光学显微镜观察细胞形态特征;免疫组化方法鉴定VSMC特异性蛋白α-SMA的表达;RT—PCR方法检测VSMC特异性蛋白α-SMA和SM22α的mRNA表达水平。结果：本方法分离原代VSMC,3d后可见长梭状VSMC从组织块边缘长出,7d后可以进行传代。光学显微镜观察显示,分离细胞具有平滑肌细胞（smooth muscle cell,SMC）形态特征;免疫组化分析显示,分离细胞α—SMA蛋白表达阳性;RT—PCR分析显示,分离细胞中α-SMA和SM22α在mRNA水平高表达。结论：本实验成功建立了一种简便、快捷、高效从组织块分离培养VSMC的改良方法,为快速获得原代VSMC提供了一种适用的方法技术。     

开设综合性生化实验提高学生的综合实验技能与创新能力

探索综合性实验对医学本科生综合实验技能与创新能力的培养功能,为培养高素质医学人才提供实验依据。我们对本校2005级医学本科各专业2125名学生开设了综合性实验—"血清γ-球蛋白的分离纯化与鉴定",并对实验设计思路、教学效果反馈信息等进行了分析,以期总结出综合实验提高本科学生综合实验技能与创新能力的培养功能与方法。通过对218份调查问卷分析表明,综合实验能巩固课堂理论知识,提高学生综合实验技能与创新能力,为学生今后从事相应专业的研究奠定坚实基础,得到了学生的普遍认同。     

50例前臂背侧高位损伤致伸指、伸拇功能障碍重建的临床体会

目的分析前臂背侧高位肌肉损伤一期手术修复后伸指、伸拇功能障碍原因,提高对前臂背侧高位肌肉损伤恢复机理的认识,保证手部伸指、伸拇正常功能.方法回顾性分析2010-2012年我科收治50例前臂背侧近端开放性外伤患者(术中探查排除桡神经深支损伤),46例肌肉损伤患者同时出现伸指、伸拇功能障碍,占92%,2例肌肉损伤患者出现伸指功能障碍,占4%,2例肌肉损伤患者出现伸拇功能障碍,占4%;而后都行功能重建恢复伸指、伸拇功能,术后上肢石膏托外固定,1月左右行功能锻炼.结果50例患者功能重建伤口均愈合良好,随访各指背伸功能良好,伸腕功能无影响或影响不是很大.结论前臂背侧高位开放性外伤致肌肉损伤,一期术后肌肉伸缩功能障碍导致伸指、伸拇功能障碍时,其中拇指功能最为重要,占整个手部功能的50%[1]左右,二期手术行功能重建尤为重要.可用收缩功能较好的桡侧腕长伸肌移位修复拇长伸肌腱的伸拇功能,桡侧腕屈肌移位修复指伸肌腱的伸指功能,将收到良好的效果.