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1.
C Niek van Dijk Ben Willem J. Mol Liesbeth S. L. Lim Rene K. Marti Patrick M. M. Bossuyt 《Acta orthopaedica》1996,67(6):566-570
We prospectively enrolled 160 consecutive patients with inversion trauma of the ankle in a diagnostic protocol that included physical examination within 2 days and at 5 days after trauma, arthrography, stress radiography, and ultrasonography. 135 patients had pathological lateral ligament laxity on the later physical examination or lateral ligament rupture diagnosed on arthrography and they were operated on. 122 of these patients had ligament ruptures.
At clinical follow-up after a minimum of half a year, all of the patients who were not operated on had stable joints without signs of previous ligament ruptures.
Delayed physical examination at 5 days after the injury led to the highest overall sensitivity (96%) and specificity (84%) for the detection of a ligament rupture. Additional diagnostic procedures, at a considerable cost, yielded little additional information. 相似文献
At clinical follow-up after a minimum of half a year, all of the patients who were not operated on had stable joints without signs of previous ligament ruptures.
Delayed physical examination at 5 days after the injury led to the highest overall sensitivity (96%) and specificity (84%) for the detection of a ligament rupture. Additional diagnostic procedures, at a considerable cost, yielded little additional information. 相似文献
2.
Liesbeth Vandenput Fernand Labrie Dan Mellstr?m Charlotte Swanson Thomas Knutsson Ralph Peeker Osten Ljunggren Eric Orwoll Anna L Eriksson Jan-Erik Damber Claes Ohlsson 《Journal of bone and mineral research》2007,22(2):220-227
Androgens are important regulators of bone and prostate health in elderly men. The role of serum levels of glucuronidated androgen metabolites as predictors of BMD and prostate volume in men is unclear. We show that specific glucuronidated androgen metabolites predict BMD and prostate volume in elderly men. INTRODUCTION: Androgens are important regulators of bone and prostate health in elderly men. Local synthesis and degradation of androgens are likely to be important parameters of biological action of androgens in androgen-responsive tissues. The aim of this study was to determine the role of serum levels of glucuronidated androgen metabolites as predictors of BMD and prostate volume in elderly men. MATERIALS AND METHODS: A subsample of the population-based Swedish part of the MrOS study (n = 631, average age = 75.9 years) was investigated. Bone parameters were measured using DXA. Serum levels of total testosterone (T) and dihydrotestosterone (DHT) were measured by gas chromatography/mass spectroscopy (GC-MS); androstane-3alpha,17beta-diol-3glucuronide (3G) and androstane-3alpha,17beta-diol-17glucuronide (17G) were measured by liquid chromatography/mass spectroscopy. Prostate volume (n = 159) was measured by transrectal ultrasound. RESULTS: The general pattern is that two of the glucuronidated androgen metabolites, namely 17G and 3G, are stronger positive predictors of BMD than the bioactive androgens (T and DHT). In addition, 17G is a clear positive predictor of prostate volume, explaining 4.5% of the variance in prostate volume, whereas the bioactive androgens do not display any association with prostate volume. CONCLUSIONS: Serum levels of specific glucuronidated androgen metabolites predict BMD and prostate volume in elderly men. Future studies should determine if the glucuronidated androgen metabolites also reflect other biological correlates of androgenic activity, including prostate cancer, and if low levels might be a marker of general androgen deficiency in men. 相似文献
3.
van Eden W Hauet-Broere F Berlo S Paul L van der Zee R de Kleer I Prakken B Taams L 《International reviews of immunology》2005,24(3-4):181-197
Immunization with microbial or mammalian stress proteins or heat-shock proteins in models of experimental autoimmunity has been observed to lead to increased disease resistance. Furthermore, such immunization has been proposed to result in the induction and expansion of T cells that suppress disease upon transfer. Comparisons of microbial heat-shock proteins with other conserved immunogenic proteins of bacterial origin have indicated a unique capacity for heat-shock proteins to induce a regulatory phenotype in T cells, such as reflected by the production of IL10. Also, studies in children with chronic arthritis have indicated that T-cell responses to heat-shock proteins are associated with a benign course of the disease and with remission. Furthermore, in patients, heat-shock-protein-(HSP-) activated T cells were shown to display regulatory phenotypes consistent with CD4+ CD25+ T regulatory cells. 相似文献
4.
P. Gilon Y. Miura J. C. Henquin J. Tytgat P. Daenens V. Decostre G. Maréchal S. M. Brichard D. J. Becker B. Reul L. N. Ongemba V. Rousseau W. Eechaute W. Dhooghe P. Calders N. C. Gao E. Lacroix J. Weyne J. Kaufman S. Tomasovic F. Frankenne A. Boland D. Delapierre D. Marechal A. Dresse O. Feron M. Wibo M. Maleki L. Zheng F. Kolar T. Godfraind K. Paemeleire L. Leybaert C. Lambillotte M. Nenquin E. Wechsung A. Houvenaghel G. Mancuso E. Tirelli S. Vandenput D. Votion D. H. Duvivier T. Art P. Lekeux H. D. Duvivier B. S. Kelemen E. Van Erck I. Mountian L. Missiaen W. Van Driessche 《Pflügers Archiv : European journal of physiology》1996,432(4):R139-R144
5.
Rooms L Reyniers E van Luijk R Scheers S Wauters J Ceulemans B Van Den Ende J Van Bever Y Kooy RF 《Human mutation》2004,23(1):17-21
Subtelomeric rearrangements are responsible for 5% to 10% of cases of unexplained mental retardation. Despite their clinical relevance, methods to screen for these cytogenetically invisible abnormalities on a routine base are scarce. We screened patients with idiopathic mental retardation for subtelomeric aberrations using multiplex ligation-dependent probe amplification (MLPA). This recently developed technique is based on PCR amplification of ligated probes hybridized to chromosome ends. Currently, 41 telomeres can be screened in just two multiplex reactions. Four subtelomeric rearrangements (5.3%) were detected in a group of 75 patients with mild to severe mental retardation in combination with dysmorphic features and/or a familial history of mental retardation: two terminal 1p deletions, a terminal 1q deletion, and a terminal 3p deletion. Deletions could be verified by FISH and marker analysis. In one case the MLPA indicated a terminal 21q deletion due to a 3-bp deletion at the site of the probe, giving a false-positive rate of 1.3%. This study demonstrates that MLPA is a fast and reliable screening method, potentially suitable for use in routine diagnostics. 相似文献
6.
Amanda Hall Anthony Hayes Liesbeth Brown Ross Tubo Bruce Caterson 《International journal of experimental pathology》2004,85(4):A63-A64
Introduction The aims of the current study were to (i) tissue engineer a cartilage graft with structural and biochemical properties of native articular cartilage in vivo, with potential for use in cartilage repair technologies and (ii) utilize this model as a test system to evaluate the efficiency of novel therapeutics for future research into cartilage metabolism in health and disease. Materials and methods Articular cartilage was harvested from hock joints of (young) 7‐day and (old) 18‐month bovine sources. Cells were isolated by enzymatic digestion and seeded at a range of cell densities (2, 4, 6, 8, 10 and 12 × 106 cells/insert) into type II collagen‐coated Millipore filter inserts and cultured as described previously ( Kandel et al. 1995 ). In order to mimic a catabolic effect on cartilage, some samples were treated with IL‐1α (10 ng/ml) for 24 h in the absence or presence of experimental drugs. Proteoglycan (PG) release, detectable in the medium, was analysed by colorimetric assay ( Farndale et al. 1986 ). At the end of the culture period, cartilage grafts were fixed, sectioned and stained with Alcian Blue or immuno‐fluorescently labelled with a panel of monoclonal antibodies recognizing several components of the graft extracellular matrix. Results Full‐depth chondrocytes from both young and old bovine sources produced a stratified hyaline tissue with distinct zones after 2 weeks in culture. These zones approximated to the surface, middle and deep zones that characterize native articular cartilage in vivo. Increased culture time and seeding density produced cartilage of an increased thickness and cellularity, respectively. Grafts produced from young cartilage contained approximately 3 times more sulfated PG than grafts produced from an old cartilage, indicating an increased matrix secretion in these cultures. Histologically, the old grafts were also thinner and more weakly stained with Alcian Blue, indicating a lower sulfated PG content. Addition of IL‐1α to the cultures resulted in a dramatic PG release from the cartilage grafts, manifest histologically as a loss of Alcian Blue staining in the upper third of the cartilage tissue. Immunofluorescent staining identified subtle changes in matrix composition and in the structure and catabolism of matrix proteoglycans in response to both IL‐1a and the experimental drugs tested. Discussion The grafts produced had many structural and biochemical similarities to articular cartilage in vivo. These grafts may better integrate with the host cartilage in cartilage repair procedure. This culture system also provides ideal conditions to analyse the response of engineered grafts to catabolic factors that occur in the arthritic joint, along with ideal conditions for research into drug therapies. Advantages of this culture system, in comparison with an explant system, are that effects can be analysed within a 24‐h period. Future work will include applying fatty acids, modified glucosamine and some Asian herbal remedies to this culture system and analysing their potential chondroprotective effects. 相似文献
7.
Inge Van de Walle Karen Silence Kevin Budding Liesbeth Van de Ven Kim Dijkxhoorn Elisabeth de Zeeuw Cafer Yildiz Sofie Gabriels Jean-Michel Percier Johanna Wildemann Jan Meeldijk Peter J. Simons Louis Boon Linda Cox Rob Holgate Rolf Urbanus Henny G. Otten Jeanette H.W. Leusen Peter Boross 《The Journal of allergy and clinical immunology》2021,147(4):1420-1429.e7
8.
Van der Veen Jacoba Te Wierik Gerrit H. P. Van der Wal Liesbeth Eissens Anko C. Lerk Coenraad F. 《Pharmaceutical research》1994,11(4):499-502
Amylodextrin is a linear dextrin and can be produced by enzymatic hydrolysis of the -1,6 glycosidic bonds of amylopectin. Tablets compacted from pure amylodextrin showed good binding properties and did not disintegrate in aqueous media. Extended and decreasing drug release rates were found for tablets of 300 mg with a diameter of 9 mm containing 70% amylodextrin and 30% theophylline monohydrate, when compacted at 5 kN. Almost-constant drug release rates were obtained for these tablets when compacted at 10 or 15 kN. Nearly constant drug release rates were also shown for amylodextrin tablets with a drug load up to 75% compacted at 10 kN. Both release rate and release profile could be adjusted by selecting tablet thickness and incorporation of either lactose as a highly soluble excipient or talc as a hydrophobic excipient. 相似文献
9.
Androgens and osteoporosis 总被引:3,自引:0,他引:3
Androgen receptors are present in relevant numbers in osteoblasts. Stimulation of androgen receptors in osteoblastic bone marrow stromal cells inhibits the differentiation of osteoclasts in the bone marrow cavity. Androgens not only inhibit osteoclastogenesis but also increase cortical bone formation mainly by stimulating periosteal bone formation. Clinically, androgen action is crucial for the gain of bone mass during puberty and the maintenance of bone mass after puberty. Therefore, androgen replacement is necessary in hypogonadal men. However, the role of androgen replacement in partial androgen deficiency still remains unclear. Thus far, only testosterone has established its role in androgen replacement. However, further clinical and basic research should better define the selective role of androgen versus oestrogen receptor stimulation in male skeletal homeostasis. 相似文献
10.
Katrien De Jaeger M.D. M.Sc. Yvette Seppenwoolde Ph.D. Harm H. Kampinga Ph.D. Liesbeth J. Boersma M.D. Ph.D. Jos S. A. Belderbos M.D. Joos V. Lebesque M.D. Ph.D. 《International journal of radiation oncology, biology, physics》2004,58(5):27-1387
PURPOSE: In dose-escalation studies of radiotherapy (RT) for non-small-cell lung cancer (NSCLC), radiation pneumonitis (RP) is the most important dose-limiting complication. Transforming growth factor-beta1 (TGF-beta1) has been reported to be associated with the incidence of RP. It has been proposed that serial measurements of plasma TGF-beta1 can be valuable to estimate the risk of RP and to decide whether additional dose-escalation can be safely applied. The aim of this study was to evaluate prospectively the time course of TGF-beta1 levels in patients irradiated for NSCLC in relation to the development of RP and dose-volume parameters. METHODS AND MATERIALS: Plasma samples were obtained in 68 patients irradiated for medically inoperable or locally advanced NSCLC (dose range, 60.8-94.5 Gy) before and 4, 6, and 18 weeks after the start of RT. Plasma TGF-beta1 levels were determined using a bioassay on the basis of TGF-beta1-induced plasminogen activator inhibitor-1 expression in mink lung cells. All patients underwent chest computed tomography scans before RT that were repeated at 18 weeks after RT. The computed tomography data were used to calculate the mean lung dose (MLD) and to score the radiation-induced radiologic changes. RP was defined on the basis of the presence of either radiographic changes or clinical symptoms. Symptomatic RP was scored according to the Common Toxicity Criteria (Grade 1 or worse) and the Southwestern Oncology Group criteria (Grade 2 or worse). Multivariate analyses were performed to investigate which factors (pre- or posttreatment TGF-beta1 level, MLD) were associated with the incidence of RP. To improve our understanding of the time course of TGF-beta1 levels, we performed a multivariate analysis to investigate which factors (pre-RT TGF-beta1 level, MLD, RP) were independently associated with the posttreatment TGF-beta1 levels. RESULTS: The pre-RT TGF-beta1 levels were increased in patients with NSCLC (median 21 ng/mL, range, 5-103 ng/mL) compared with healthy individuals (range, 4-12 ng/mL). On average, the TGF-beta1 levels normalized toward the end of treatment and remained stable until 18 weeks after RT. In 29 patients, however, TGF-beta1 was increased at the end of RT with respect to the pre-RT value. The multivariate analyses revealed that the MLD was the only variable that correlated significantly with the risk of both radiographic RP (p = 0.05) and symptomatic RP, independent of the scoring system used (p = 0.05 and 0.03 for Southwestern Oncology Group and Common Toxicity Criteria systems, respectively). The TGF-beta1 level at the end of RT was significantly associated with the MLD (p <0.001) and pre-RT TGF-beta1 level (p = 0.001). CONCLUSION: The MLD correlated significantly with the incidence of both radiographic and symptomatic RP. The results of our study did not confirm the reports that increased levels of TGF-beta1 at the end of RT are an independent additional risk factor for developing symptomatic RP. However, the TGF-beta1 level at the end of a RT was significantly associated with the MLD and the pre-RT level. 相似文献