Some aspects of adult living donor liver transplantation:small-for-size graft and ABO mismatch

Living donor liver transplantation (LDLT) was first performed for pediatric patients, for whom the gap between demand and supply of     

Evaluation of Helicobacter pylori stool antigen test before and after eradication therapy

BACKGROUND AND AIM: The Helicobacter pylori stool antigen (HpSA) test is useful for initial diagnosis of H. pylori infection, but there is disagreement regarding its diagnostic accuracy after eradication therapy. The aim of the present study was to evaluate the diagnostic accuracy of the HpSA test before and after eradication therapy. METHODS: One hundred and thirty-six patients underwent upper gastrointestinal endoscopy with biopsies for the diagnosis of H. pylori infection using culture, histology and the rapid urease test. Fifty-four H. pylori-positive patients were treated with 1-week triple therapy. Six to 10 weeks after the end of therapy, the patients underwent re-endoscopy and received the same biopsy-based methods. In addition, the 13C-urea breath test was performed. The HpSA test was performed before and 6-10 weeks after the end of therapy. In 23 patients, the HpSA test was also performed at the end of therapy. RESULTS: Before therapy, the sensitivity and specificity of the HpSA test was 98.3% (95% confidence interval (CI): 95.9-100%) and 95.0% (95% CI: 75.1-99.9%), respectively. At the end of therapy, the HpSA tests were all negative both for eradication and non-eradication patients. The sensitivity and specificity of the HpSA test after eradication therapy were 90% (95% CI: 55.5-99.7%) and 97.7% (95% CI: 93.3-100%), respectively. CONCLUSIONS: The HpSA test is a useful method for the diagnosis of H. pylori infection before and after eradication therapy.     

A comparative study on mouse MHC class I sequences detected in Schistosoma japonicum recovered from BALB/c (H-2d) and C57BL/6 (H-2b) mice

The mouse major histocompatibility complex (MHC) class I sequence was detected in all the 8-week-old Schistosoma japonicum recovered from BALB/c (H-2d) and C57BL/6 (H-2b) mice by in situ polymerase chain reaction (in situ PCR). The signals of the mouse class I MHC sequence were observed in the nuclei of the mesenchymal and reproductive cells of 8-week-old S. japonicum. Furthermore, the class I MHC sequence was detected in each DNA extracted from S. japonicum cercariae maintained in BALB/c and C57BL/6 mice by nested PCR. To prove both horizontal and vertical transmission of this sequence in schistosomes, we have used cercariae obtained from parasites maintained in BALB/c mice to infect C57BL/6 and BALB/c mice, and vice versa. The MHC sequences from adult worms were compared to the cercarial MHC and host MHC sequences. Nucleotide sequence comparisons between adult worm DNA, host (H-2d and H-2b mice) DNA and cercarial DNA used for the infection suggested that the sequence of mouse class I MHC was incorporated into schistosome adults and inherited throughout their life-cycle.     

Pseudomesotheliomatous lung cancer mimicking mesothelioma on 18F-FDG PET/CT images: report of 2 cases

The authors report two cases of pseudomesotheliomatous lung cancer (PLC) detected by ¹⁸F-FDG PET/CT scan. ¹⁸F-FDG PET/CT clearly revealed the extent of the disease in both cases, a case of adenocarcinoma of the lung and a case of squamous cell carcinoma of the lung. Intense ¹⁸F-FDG uptake by the diffusely thickened pleurae and primary lesion was observed in both cases, and increased ¹⁸F-FDG uptake by a pelvic bone metastasis was observed in the case of squamous cell carcinoma. Although PLC is indistinguishable from malignant pleural mesothelioma on ¹⁸F-FDG PET/CT scans, ¹⁸F-FDG PET/CT was helpful in identifying the primary focus of the PLCs and in staging the disease. Diagnostic image interpreters should be familiar with the ¹⁸F-FDG PET/CT findings in PLC.     

小鼠胸腺巨噬细胞吞噬周期的超微结构及细胞化学的观察

本实验对正常小鼠胸腺巨噬细胞内外源性物质消化(异噬)和内源性物质消化(自噬)过程中的形态学改变和酸性磷酸酶(AcPase)活性进行了观察。发现在巨噬细胞吞噬过程中,其溶酶体系有周期性改变。根据这一周期性改变本文提出了吞噬周期的概念。此周期可分为异噬阶段和自噬阶段。异噬阶段主要表现为吞噬机能增强和形成大量不规则(变形)的异噬溶酶体(HPL)。然后。通过变形HPL的发芽和缢缩形成许多小溶酶体.在自噬阶段,许多小溶酶体被变形HPL包裹。应该指出,自噬阶段巨噬细胞的自噬是HPL的自身吞噬现象。     

镇眩饮对椎动脉缺血及正常大鼠脑血流量的影响

目的:探讨镇眩饮对大鼠椎动脉缺血的治疗作用;对大鼠4VO 脑缺血的预防作用及对正常脑的影响。方法:采用电凝双侧椎动脉(椎2VO)模型及 Pulsinelli 4VO 模型。用~(123)I-IMP 示踪脑血流。结果:椎2VO 模型除大脑皮质外的脑各部位及全脑血流量下降(P<0.05)。镇眩饮0.3g/kg 可使其恢复正常(P<0.05～0.01)。镇眩饮日剂量0.3g/kg 和1g/kg,连续1周,未观察到有效预防作用。正常大鼠用镇眩饮日剂量0.3g/kg,24h 及1周后,均对全脑血流量无明显影响。结论:椎2VO 是更接近于临床椎-基底动脉缺血性眩晕的模型,镇眩饮对其的治疗效果与对 PuIsinelli 4VO 模型相同。镇眩饮是比较理想的脑血流改善剂,不会引起脑内“窃血”现象。