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排序方式: 共有319条查询结果,搜索用时 15 毫秒
1.
我国登革2型病毒43株包膜E蛋白基因的特征 总被引:2,自引:0,他引:2
对我国1987年流行的登革2型病毒43株包膜E蛋白基因的核苷酸序列进行了分析。结果表明登革2型病毒43株包膜E蛋白基因核苷酸序列含1485个核苷酸,编码495个氨基酸,并就其核苷酸序列及其相应的氨基酸序列与其它的登革2型病毒株进行了比较,发现核苷酸序列与我国1985年分离的登革2型病毒04株,新几内亚C株(NGC),牙买加株1409(JAM)和马来西亚当地流行株M1(登革出血热)、血2(登革休克综合征)、M3(登革热)同源性分别是95.8%、94.6%、97.5%、925%、92.7%和939%,氨基酸序列的同源性分别是94.3%、94.3%、96.0%、93.7%、93.7%和91.5%,推断出的氨基酸序列显示出12个保守的半胱氨酸残基和两个潜在的糖基化位点,分别位于Asn-67和Asn-153位。 相似文献
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应用长链RT-PCR法扩增我国登革2、4型病毒株全长cDNA 总被引:3,自引:1,他引:3
目的:采用长链RT-PCR技术扩增登革2型及4型病毒基因组全长cDNA,为构建登革病毒全长cDNA克隆、表达,深入阐明致病机理及探索新型疫苗奠定基础。方法:根据已测定的登革2、4型病毒全基因组序列,设计上下游引物。从感染登革病毒的乳鼠脑中提取病毒基因组RNA,采用长链RT-PCR技术进行扩增。为检验扩增产物的特异性,以PCR产物为模板扩增覆盖基因组的10个片段。将含有复杂二级结构的5′非编码区扩增片段,在377A型自动测序仪进行序列分析。结果:扩增出登革2、4型病毒基因组全长近11kb cDNA分子,非编码区测序结果表明扩增产物为登革2、4型病毒所特有。结论:利用长链RT-PCR首次成功扩增出登革病毒全长cDNA分子。 相似文献
4.
Developing more resilient and sustainable physical infrastructure increases the demand for sustainable materials and strengthening approaches. Many investigations into strengthening RC beam structures have used either externally bonded (EB) or near-surface mounted (NSM) systems with synthetic fibre reinforced polymer composites. These synthetic fibres are unsustainable since they involve the use of nonrenewable resources and a large amount of energy. Research shows that natural fibre reinforced polymer (NFRP) composites may be an alternative to synthetic FRP composites in the strengthening of concrete beams. However, there is limited literature that validates their performance in various structural applications. Hence, the purpose of this paper is to explore the advances, prospects, and gaps of using EB/NSM NFRP techniques in strengthening concrete beams to provide areas for future research directions. The NSM FRP technique provides improved strengthening effects and mitigates the concerns associated with the EB system, based on a wider range of applications using synthetic FRPs. However, the NSM NFRP strengthening technique has been underutilized, though the EB NFRP system has been more commonly explored in reviewed studies. The knowledge gaps and areas for proposed future research directions are essential in developing work in emerging NFRPs and strengthening techniques for sustainable infrastructure. 相似文献
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6.
Lisa B. Haddad Leah Kathleen Machen Sarah Cordes Brian Huylebroeck Augustina Delaney Igho Ofotokun 《AIDS care》2016,28(4):455-459
Little is known regarding family planning desires among women living with HIV in the United States. This study aimed to identify factors influencing desire for children in the future among HIV-infected women in Atlanta, Georgia. HIV-infected women ages 18–45 completed an ACASI (audio computer-assisted self-interview) questionnaire. Chi-square, t-tests, and multivariate logistic regression evaluated factors associated with desire for future children. Of 181 participants, 62 (34.3%) expressed desire for children in the future, with increased desire among younger women (age <26) and those with seronegative partners. Concerns for horizontal and vertical HIV transmission were deterrents to future childbearing. Condom use and overall knowledge of transmission risk was low. Over a third of women desiring a child never discussed their desire with a physician. Misinformation regarding HIV transmission risks persists and is a notable concern influencing desire for children. Providers should reassess family planning desires regularly through integrated HIV care. 相似文献
7.
Fabio Paglialonga Claus Peter Schmitt Rukshana Shroff Karel Vondrak Christoph Aufricht Alan Rees Watson Gema Ariceta Michael Fischbach Gunter Klaus Tuula Holtta Sevcan A. Bakkaloglu Alexandra Zurowska Augustina Jankauskiene Johan Vande Walle Betti Schaefer Elizabeth Wright Roy Connell Alberto Edefonti 《Pediatric nephrology (Berlin, Germany)》2015,30(1):103-111
8.
Stephanie Dufek Tuula Holtta Michel Fischbach Gema Ariceta Augustina Jankauskiene Rimante Cerkauskiene Claus Peter Schmitt Betti Schaefer Christoph Aufricht Elizabeth Wright Constantinos J. Stefanidis Mesiha Ekim Sevcan Bakkaloglu Günter Klaus Aleksandra Zurowska Karel Vondrak Johan Vande Walle Alberto Edefonti Rukshana Shroff 《Pediatric nephrology (Berlin, Germany)》2015,30(11):2021-2027
9.
Ekwempu Adaobi Ifeoma Ede Folashade Rebecca Ojo Oyekunle Ezekiel Ofojekwu Mary-Jane Essien Cosmas Uyime Edeh N. Obiageri Adeshiyan Tola Damen James 《Nigerian medical journal》2015,56(6):425-428
Background:
Continuing professional development (CPD) in Medical Laboratory Scientists (MLS) is aimed at equipping laboratory professionals with the necessary skills to enhance practice. The laboratory scientists are usually the first contact between the patient and health care system in aspects of diagnosis and monitory of diseases. As such, it becomes imperative to assess the knowledge of laboratory personnel regarding CPD.Materials and Methods:
Self-administered questionnaires were distributed to 200 laboratory personnel''s attending the maiden CPD workshop organized by the Association of MLS in Jos the Plateau state capital.Results:
One hundred and thirty-five (82 males and 53 females) of the 200 administered questionnaires were returned. Only 32 of them (23.7%) attended CPD program in the last 1 year with 10 (7.5%) engaging in online CPD. Five (3.7%) of the respondents had the privilege to attend an international CPD. Majority (95.2%) of the respondents identified CPD as an essential component of professional career development. Lack of sponsorship was identified as a major setback in CPD efficiency by 93.8% of respondents. About 58 (46.4%) noted that poor attendance in CPD workshops was due to unavailability of policy guideline for CPD. One hundred and twenty (95.2%) of respondents had an aim of improving their skills after attending CPD workshops.Conclusion:
The overall attitude of Nigerian MLS toward attending CPD workshop is poor; however, the knowledge regarding the importance of CPD is adequate. There exists a gap between sponsorship for CPD by various institutions and MLS. 相似文献10.